Androgen Action: Methods and Protocols (Methods in Molecular Biology)

Androgens play a critical role in the development and maintenance of the male reproductive system and affect important physiological processes and pathological conditions, including the homeostasis of the normal prostate and prostate cancer. Androgen Action: Methods and Protocols is designed to provide a tool box to study various phases of androgen action, from its entry to the cell to the phenotypic response that the cell mounts, with up-to-date techniques for biochemists, molecular biologists, cell biologists, geneticists, and pathologists. The volume opens with a brief review of the research history on androgen action and prostate carcinogenesis, followed by chapters that cover state-of-the-art methods to determine androgen levels in biological tissues and fluids, experimental procedures to study the various aspects of androgen receptor activity, and methodology to study salient examples of interactions between androgen signalling and other major signalling pathways in cells. Written in the successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and easily accessible, Androgen Action: Methods and Protocols provides a comprehensive overview of, and practical guidance on, the diverse methodologies that are propelling androgen action research forward, both for normal physiology as well as in disease states. Part I. Reviews   1. Molecular Mechanisms of Androgen Action - A Historical Perspective             Albert O. Brinkmann   2. Androgen Action during Prostate Carcinogenesis             Diping Wang and Donald J. Tindall   Part II. Analysis of Androgens   3. Androgen Quantitation in Prostate Cancer Tissue using Liquid Chromatography Tandem Mass Spectrometry             Mark Titus and Kenneth B. Tome   4. Ligand Competition Binding Assay for the Androgen Receptor             Clmentine Fau, Leggy A. Arnold, Aaron Kosinski, and R. Kiplin Guy   Part III. Androgen receptor biology   5. Analysis of Androgen Receptor Activity by Reporter Gene Assays             Harri Makkonen, Tiina Jskelinen, Miia Rytinki, and Jorma J. Palvimo   6. Identification and Characterization of Androgen Response Elements             Reinhilde De Bruyn, Rita Bollen, and Frank Claessens   7. In vitro and In vivo Silencing of the Androgen Receptor             Helen Cheng, Eric Leblanc, and Paul S. Rennie   8. Analysis of Interdomain Interactions of the Androgen Receptor             Elizabeth M. Wilson   9. Methods to Study Dynamic Interaction of Androgen Receptor with Chromatin in Living Cells             Hatice Zeynep Kirli and Fahri Saatcioglu   10. FRET Analysis of Androgen Receptor Structure and Biochemistry in Living Cells             Fred Schaufele   Part IV. Analysis of Androgen Receptor Modifications and Interactions   11. Analysis of Nuclear Receptor Acetylation             Chenguang Wang, Michael Powell, Lifeng Tian, and Richard G. Pestell   12. Analysis of Androgen Receptor SUMOylation             Miia M. Rytinki, Sanna Kaikkonen, Pivi Sutinen, and Jorma J. Palvimo   13. Analysis of Ligand-specific Co-repressor Binding to the Androgen Receptor             Claudia Gerlach, Daniela Roell, and Aria Baniahmad   14. Detection of Ligand-selective Interactions of the Human Androgen Receptor by SELDI-MS-TOF             Thomas Linke, Martin Scholten, and Aria Baniahmad   Part V. Recent Advances in Studying Androgen Receptor Signalling   15. Global Identification of Androgen Response Elements             Charles E. Massie and Ian G. Mills   16. Tissue Specific Knockout of Androgen Receptor in Mice             Tzuhua Lin, Shuyuan Yeh, and Chawnshang Chang   17. Methodology to Investigate Androgen-sensitive and Castration-resistant Human Prostate Cancer Xenografts in Preclinical Setting             Holly M. Nguyen and Eva Corey   18. Automated Microscopy and Image Analysis for Androgen Receptor Function             Sean M. Hartig, Justin Y. Newberg, Michael J. Bolt, Adam T. Szafran, Marco Marcelli, and Michael A. Mancini   Part VI. Cross-talk of Androgen and Other Signalling Pathways   19. Androgen Regulation of ETS-gene Fusion Transcripts in Prostate Cancer             Delila Gasi and Jan Trapman    20. Regulation of Apoptosis by Androgens in Prostate Cancer Cells             Yke Jildouw Arnoldussen, Ling Wang, and Fahri Saatcioglu   21. Analysis of Androgen Receptor Rapid Actions in Cellular Signaling Pathways: Receptor /Src Association             Antimo Migliaccio, Gabriella Castoria, and Ferdinando Auricchio   22. Analysis of Androgen-induced Increase in Lipid Accumulation in Prostate Cancer Cells             Jrgen Sikkeland, Torstein Lindstad, and Fahri Saatcioglu