Mammalian Synthetic Promoters (Methods in Molecular Biology)

This volume details methods that will aid in the selection of promoter sequences and vector components and methods for the assembly and testing of synthetic promoters with examples of their application. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Mammalian Synthetic Promoters: Methods and Protocols aims to aid researchers that are new to the field of synthetic promoters and inspire new developments. 1.         Initial Considerations before Designing a Promoter Construct David Gould   Part I: Monitoring Promoter Interactions and Activity 2          Demonstrating Interactions of Transcription Factors with DNA by Electrophoretic Mobility Shift Assay Nasim Yousaf and David Gould   3.         Chromatin Immunoprecipitation and Quantitative Real-time PCR to Assess Binding of a Protein of Interest to Identified Predicted Binding Sites within a Promoter Jordan Read   4.         Secreted Reporters for Monitoring Multiple Promoter Function Ghazal Lashgari, Rami S. Kantar, and Bakhos A. Tannous   5.         Bioluminescence Monitoring Of Promoter Activity In Vitro and In Vivo Juliette MKM Delhove, Rajvinder Karda, Kate E Hawkins, Simon N Waddington, and Tristan R McKay   6.         Monitoring Promoter Activity by Flow Cytometry Taher E. I. Taher   Part II: Promoter Design and Applications 7.         Functional Screening of Core Promoter Activity Dan Y. Even, Adi Kedmi, Diana Ideses, and Tamar Juven-Gershon   8.         Bioinformatically Informed Design of Synthetic Mammalian Promoters Michael L. Roberts, Polyxeni Katsoupi, Vivian Tsevleki, and Era Taoufik   9.         Synthetic Tumor Specific Promoters for Transcriptional Regulation of Viral Replication M. Veronica Lopez, Eduardo. G. Cafferata, Diego. L. Viale, and Osvaldo Podhajcer   10.       Constructing Strong Cell-Type-Specific Promoters through Informed Design Adam J. Brown and David C. James   11.       PCR Assembly of Synthetic Promoters Hodan Mohamed and David Gould   Part III: Regulated Promoter Approaches 12.       The Tetracycline Responsive System Nasim Yousaf and David Gould   13.       Light-responsive Promoters Maximilian Hrner, Konrad Mller, and Wilfried Weber   14.       A Simple Method for Constructing Artificial Promoters Activated in Response to Ultrasound Stimulation Ryohei Ogawa, Go Kagiya, Akihiko Watanabe, Akihiro Morii, Zheng-Guo Cui, and Takashi Kondo 15.       Promoter Activation with Electromagnetism Abraham O. Rodrguez-De la Fuente, J. Antonio Heredia-Rojas, Juan M. Alcocer-Gonzlez, Laura E. Rodrguez-Flores, Cristina Rodrguez-Padilla, and Reyes S. Tamz-Guerra   16.       Application of Synthetic Tumor Specific Promoters Responsive to the Tumor Microenvironment Eduardo G Cafferata, M Vernica Lpez, Felipe J Nuez, Mara Agostina Russo Maenza, and Osvaldo L Podhajcer   Part IV: Combining Promoters in Synthetic Circuits 17.       A Modular Approach to Building Complex Synthetic Circuits Yinqing Li and Ron Weiss   18.       Computational Sequence Design with R2oDNA Designer James T. MacDonald and Velia Siciliano   19.       Design of Synthetic Promoters for Gene Circuits in Mammalian Cells Pratik Saxena, Daniel Bojar, and Martin Fussenegger