Description
Divided into two convenient sections, Protein Kinase Technologies collects contributions from experts in the field examining recent methodologies and techniques generally applicable to protein kinase research as well as to individual protein kinases which require special attention in neuroscience. These chapters will not only be practical instructions useful for readers’ daily work in setting up and performing research but also thought-provoking and enjoyable reviews of recent advancements of individual protein kinases in neuroscience. Written for the highly successful Neuromethods series, this work contains the kind of detailed description and implementation advice that is crucial for getting optimal results. Authoritative and accessible, Protein Kinase Technologies seeks to foster cross-talk among investigators who study different protein kinases, and will also be beneficial for the entry of new investigators into this pivotal field. Part I: Recent General Methodologies 1. Visualization of Protein Kinase Activities in Living Cells Kazuhiro Aoki, Naoki Komatsu, Akihiro Goto, and Michiyuki Matsuda 2. Phos-Tag Affinity Electrophoresis for Protein Kinase Profiling Eiji Kinoshita, Emiko Kinoshita-Kikuta, and Tohru Koike 3. Large-Scale Protein Phosphorylation Analysis by Mass Spectrometry-Based Phosphoproteomics Wei-Chi Ku, Naoyuki Sugiyama, and Yasushi Ishihama Part II: Individual Protein Kinases 4. Ca2+/Calmodulin-Dependent Protein Kinase II (CaMKII) Steven J. Coultrap and K. Ulrich Bayer 5. CASK: A Specialized Neuronal Kinase 15. Diacylglycerol Kinase (DGK) Konark Mukherjee 6. Cyclin-Dependent Kinase 5 (Cdk5): Preparation and Measurement of Kinase Activity 15. Diacylglycerol Kinase (DGK) Seiji Minegishi and Shin-ichi Hisanaga 7. ErbB Membrane Tyrosine Kinase Receptors: Analyzing Migration in a Highly Complex Signaling System 15. Diacylglycerol Kinase (DGK) Nicole M. Brossier, Stephanie J. Byer, Lafe T. Peavler, and Steven L. Carroll 8. The Extracellular-Signal Regulated Kinase (ERK) Cascade in Neuronal Cell Signaling 15. Diacylglycerol Kinase (DGK) Daniel Orellana, Ilaria Morella, Marzia Indrigo, Alessandro Papale, and Riccardo Brambilla 9. Glycogen Synthase Kinase-3 in Neurological Diseases 15. Diacylglycerol Kinase (DGK) Oksana Kaidanovich-Beilin and James Robert Woodgett 10. Cortical Neurons Culture to Study c-Jun N-Terminal Kinase Signaling Pathway 15. Diacylglycerol Kinase (DGK) Alessandra Sclip, Xanthi Antoniou, and Tiziana Borsello 11. MuSK: A Kinase Critical for the Formation and Maintenance of the Neuromuscular Junction 15. Diacylglycerol Kinase (DGK) Arnab Barik and Lin Mei 12. Assays for Pten-Induced Novel Kinase 1 (PINK1) and Leucine-Rich Repeat Kinase 2 (LRRK2), Kinases Associated with Parkinson’s Disease 15. Diacylglycerol Kinase (DGK) Alexandra Beilina and Mark R. Cookson 15. Diacylglycerol Kinase (DGK) 13. Imaging PKA Activation Inside Neurons in Brain Slice Preparations 15. Diacylglycerol Kinase (DGK) Marina Brito, Elvire Guiot, and Pierre Vincent 14. Imaging Oscillations of Protein Kinase C Activity in Cells 15. Diacylglycerol Kinase (DGK) Maya T. Kunkel and Alexandra C. Newton Yasuhito Shirai and Naoaki Saito 16. Trafficking of Trk Receptors Daniel Bodmer and Rejji Kuruvilla 17. Mammalian Target of Rapamycin Lukasz Swiech, Malgorzata Urbanska, Matylda Macias, Agnieszka Skalecka, and Jacek Jaworski 18. Protein Kinase G (PKG): Involvement in Promoting Neural Cell Survival, Proliferation, Synaptogenesis, and Synaptic Plasticity and the Use of New Ultrasensitive Capillary-Electrophoresis-Based Methodologies for Measuring PKG Expression and Molecular Actions Ronald R. Fiscus and Mary G. Johlfs 19. Electrophysiological Technique for Analysis of Synaptic Function of PKN1 in Hippocampus Hiroki Yasuda and Hideyuki Mukai 15. Diacylglycerol Kinase (DGK) Yasuhito Shirai and Naoaki Saito 16. Trafficking of Trk Receptors Daniel Bodmer and Rejji Kuruvilla 17. Mammalian Target of Rapamycin Lukasz Swiech, Malgorzata Urbanska, Matylda Macias, Agnieszka Skalecka, and Jacek Jaworski 18. Protein Kinase G (PKG): Involvement in Promoting Neural Cell Survival, Proliferation, Synaptogenesis, and Synaptic Plasticity and the Use of New Ultrasensitive Capillary-Electrophoresis-Based Methodologies for Measuring PKG Expression and Molecular Actions Ronald R. Fiscus and Mary G. Johlfs 19. Electrophysiological Technique for Analysis of Synaptic Function of PKN1 in Hippocampus Hiroki Yasuda and Hideyuki Mukai
