Description
Visualization of chemicals in tissues has seen incredible advances in the past several years. Visualization Techniques: From Immunohistochemistry to Magnetic Resonance Imaging provides practical advice from experts in the field as well as an excellent overview of some of the most important recent advances in visualization. This timely volume explores topics from immunohistochemistry for multiple neurochemicals, detecting expression levels of neurochemicals, following cellular processes and ionic movement, identifying polysynaptic pathways subserving physiological responses to identifying functional changes in vivo. Written for the popular Neuromethods series, this work includes the kind of detailed description and implementation advice that is crucial for getting optimal results in the lab. Meticulous and concise, Visualization Techniques: From Immunohistochemistry to Magnetic Resonance Imaging will prove invaluable for scientists seeking to gain a greater understanding of the practical skills, strengths, and pitfalls that these wonderful and exciting visualization techniques provide. 1. Multiple Immunohistochemical Labelling of Peripheral Neurons Ian L. Gibbins 2. Combined In Situ Hybridization and Immunohistochemistry in Tat Brain Tissue Using Digoxigenin Labelled Riboprobes Natasha N. Kumar, Belinda R. Bowman and Ann K. Goodchild 3. In Situ Hybridization within the CNS Tissue: Combining In Situ Hybridization with Immunofluorescence Dominic Bastien and Steve Lacroix 4. Visualizing GABAB Receptor Internalization and Intracellular Trafficking Paola Ramoino, Paolo Bianchini, Alberto Diaspro, and Cesare Usai 5. Using Total Internal Reflection Fluorescence Microscopy (TIRFM) to Visualize Insulin Action James G. Burchfield, Jamie A. Lopez, and William E. Hughes 6. Live-Cell Quantification of Mitochondrial Functional Parameters Marco Nooteboom, Marleen Forkink, Peter H.G.M. Willems, and Werner J.H. Koopman 7. Functional Imaging Using Two-Photon Microscopy in Living Tissue Ivo Vanzetta, Thomas Deneux, Attila Kaszs, Gergely Katona, and Balazs Rozsa 8. Calcium Imaging Techniques In Vitro to Explore the Role of Dendrites in Signaling Physiological Action Potential Patterns Audrey Bonnan, Benjamin Grewe, and Andreas Frick 9. Juxtacellular Labeling in Combination with Other Histological Techniques to Determine Phenotype of Physiologically Identified Neurons Ruth L. Stornetta 10. Visualization of Activated Neurons Involved in Endocrine and Dietary Pathways Using GFP-Expressing Mice Rim Hassouna, Odile Viltart, Lucille Tallot, Karine Bouyer, Catherine Videau, Jacques Epelbaum, Virginie Tolle, and Emilio Badoer 11. Use and Visualization of Neuroanatomical Viral Transneuronal Tracers J. Patrick Card and Lynn W. Enquist 12. Visualization of Thermal Changes in Freely Moving Animals Daniel M.L. Vianna and Pascal Carrive 13. Perfusion Magnetic Resonance Imaging Quantification in the Brain Fernando Calamante Ian L. Gibbins 2. Combined In Situ Hybridization and Immunohistochemistry in Tat Brain Tissue Using Digoxigenin Labelled Riboprobes Natasha N. Kumar, Belinda R. Bowman and Ann K. Goodchild 3. In Situ Hybridization within the CNS Tissue: Combining In Situ Hybridization with Immunofluorescence Dominic Bastien and Steve Lacroix 4. Visualizing GABAB Receptor Internalization and Intracellular Trafficking Paola Ramoino, Paolo Bianchini, Alberto Diaspro, and Cesare Usai 5. Using Total Internal Reflection Fluorescence Microscopy (TIRFM) to Visualize Insulin Action James G. Burchfield, Jamie A. Lopez, and William E. Hughes 6. Live-Cell Quantification of Mitochondrial Functional Parameters Marco Nooteboom, Marleen Forkink, Peter H.G.M. Willems, and Werner J.H. Koopman 7. Functional Imaging Using Two-Photon Microscopy in Living Tissue Ivo Vanzetta, Thomas Deneux, Attila Kaszs, Gergely Katona, and Balazs Rozsa 8. Calcium Imaging Techniques In Vitro to Explore the Role of Dendrites in Signaling Physiological Action Potential Patterns Audrey Bonnan, Benjamin Grewe, and Andreas Frick 9. Juxtacellular Labeling in Combination with Other Histological Techniques to Determine Phenotype of Physiologically Identified Neurons Ruth L. Stornetta 10. Visualization of Activated Neurons Involved in Endocrine and Dietary Pathways Using GFP-Expressing Mice Rim Hassouna, Odile Viltart, Lucille Tallot, Karine Bouyer, Catherine Videau, Jacques Epelbaum, Virginie Tolle, and Emilio Badoer 11. Use and Visualization of Neuroanatomical Viral Transneuronal Tracers J. Patrick Card and Lynn W. Enquist 12. Visualization of Thermal Changes in Freely Moving Animals Daniel M.L. Vianna and Pascal Carrive 13. Perfusion Magnetic Resonance Imaging Quantification in the Brain Fernando Calamante Natasha N. Kumar, Belinda R. Bowman and Ann K. Goodchild 3. In Situ Hybridization within the CNS Tissue: Combining In Situ Hybridization with Immunofluorescence Dominic Bastien and Steve Lacroix 4. Visualizing GABAB Receptor Internalization and Intracellular Trafficking Paola Ramoino, Paolo Bianchini, Alberto Diaspro, and Cesare Usai 5. Using Total Internal Reflection Fluorescence Microscopy (TIRFM) to Visualize Insulin Action James G. Burchfield, Jamie A. Lopez, and William E. Hughes 6. Live-Cell Quantification of Mitochondrial Functional Parameters Marco Nooteboom, Marleen Forkink, Peter H.G.M. Willems, and Werner J.H. Koopman 7. Functional Imaging Using Two-Photon Microscopy in Living Tissue Ivo Vanzetta, Thomas Deneux, Attila Kaszs, Gergely Katona, and Balazs Rozsa 8. Calcium Imaging Techniques In Vitro to Explore the Role of Dendrites in Signaling Physiological Action Potential Patterns Audrey Bonnan, Benjamin Grewe, and Andreas Frick 9. Juxtacellular Labeling in Combination with Other Histological Techniques to Determine Phenotype of Physiologically Identified Neurons Ruth L. Stornetta 10. Visualization of Activated Neurons Involved in Endocrine and Dietary Pathways Using GFP-Expressing Mice Rim Hassouna, Odile Viltart, Lucille Tallot, Karine Bouyer, Catherine Videau, Jacques Epelbaum, Virginie Tolle, and Emilio Badoer 11. Use and Visualization of Neuroanatomical Viral Transneuronal Tracers J. Patrick Card and Lynn W. Enquist 12. Visualization of Thermal Changes in Freely Moving Animals Daniel M.L. Vianna and Pascal Carrive 13. Perfusion Magnetic Resonance Imaging Quantification in the Brain Fernando Calamante Paola Ramoino, Paolo Bianchini, Alberto Diaspro, and Cesare Usai 5. Using Total Internal Reflection Fluorescence Microscopy (TIRFM) to Visualize Insulin Action James G. Burchfield, Jamie A. Lopez, and William E. Hughes 6. Live-Cell Quantification of Mitochondrial Functional Parameters Marco Nooteboom, Marleen Forkink, Peter H.G.M. Willems, and Werner J.H. Koopman 7. Functional Imaging Using Two-Photon Microscopy in Living Tissue Ivo Vanzetta, Thomas Deneux, Attila Kaszs, Gergely Katona, and Balazs Rozsa 8. Calcium Imaging Techniques In Vitro to Explore the Role of Dendrites in Signaling Physiological Action Potential Patterns Audrey Bonnan, Benjamin Grewe, and Andreas Frick 9. Juxtacellular Labeling in Combination with Other Histological Techniques to Determine Phenotype of Physiologically Identified Neurons Ruth L. Stornetta 10. Visualization of Activated Neurons Involved in Endocrine and Dietary Pathways Using GFP-Expressing Mice Rim Hassouna, Odile Viltart, Lucille Tallot, Karine Bouyer, Catherine Videau, Jacques Epelbaum, Virginie Tolle, and Emilio Badoer 11. Use and Visualization of Neuroanatomical Viral Transneuronal Tracers J. Patrick Card and Lynn W. Enquist 12. Visualization of Thermal Changes in Freely Moving Animals Daniel M.L. Vianna and Pascal Carrive 13. Perfusion Magnetic Resonance Imaging Quantification in the Brain Fernando Calamante James G. Burchfield, Jamie A. Lopez, and William E. Hughes 6. Live-Cell Quantification of Mitochondrial Functional Parameters Marco Nooteboom, Marleen Forkink, Peter H.G.M. Willems, and Werner J.H. Koopman 7. Functional Imaging Using Two-Photon Microscopy in Living Tissue Ivo Vanzetta, Thomas Deneux, Attila Kaszs, Gergely Katona, and Balazs Rozsa 8. Calcium Imaging Techniques In Vitro to Explore the Role of Dendrites in Signaling Physiological Action Potential Patterns Audrey Bonnan, Benjamin Grewe, and Andreas Frick 9. Juxtacellular Labeling in Combination with Other Histological Techniques to Determine Phenotype of Physiologically Identified Neurons Ruth L. Stornetta 10. Visualization of Activated Neurons Involved in Endocrine and Dietary Pathways Using GFP-Expressing Mice Rim Hassouna, Odile Viltart, Lucille Tallot, Karine Bouyer, Catherine Videau, Jacques Epelbaum, Virginie Tolle, and Emilio Badoer 11. Use and Visualization of Neuroanatomical Viral Transneuronal Tracers J. Patrick Card and Lynn W. Enquist 12. Visualization of Thermal Changes in Freely Moving Animals Daniel M.L. Vianna and Pascal Carrive 13. Perfusion Magnetic Resonance Imaging Quantification in the Brain Fernando Calamante Marco Nooteboom, Marleen Forkink, Peter H.G.M. Willems, and Werner J.H. Koopman 7. Functional Imaging Using Two-Photon Microscopy in Living Tissue Ivo Vanzetta, Thomas Deneux, Attila Kaszs, Gergely Katona, and Balazs Rozsa 8. Calcium Imaging Techniques In Vitro to Explore the Role of Dendrites in Signaling Physiological Action Potential Patterns Audrey Bonnan, Benjamin Grewe, and Andreas Frick 9. Juxtacellular Labeling in Combination with Other Histological Techniques to Determine Phenotype of Physiologically Identified Neurons Ruth L. Stornetta 10. Visualization of Activated Neurons Involved in Endocrine and Dietary Pathways Using GFP-Expressing Mice Rim Hassouna, Odile Viltart, Lucille Tallot, Karine Bouyer, Catherine Videau, Jacques Epelbaum, Virginie Tolle, and Emilio Badoer 11. Use and Visualization of Neuroanatomical Viral Transneuronal Tracers J. Patrick Card and Lynn W. Enquist 12. Visualization of Thermal Changes in Freely Moving Animals Daniel M.L. Vianna and Pascal Carrive 13. Perfusion Magnetic Resonance Imaging Quantification in the Brain Fernando Calamante Ivo Vanzetta, Thomas Deneux, Attila Kaszs, Gergely Katona, and Balazs Rozsa 8. Calcium Imaging Techniques In Vitro to Explore the Role of Dendrites in Signaling Physiological Action Potential Patterns Audrey Bonnan, Benjamin Grewe, and Andreas Frick 9. Juxtacellular Labeling in Combination with Other Histological Techniques to Determine Phenotype of Physiologically Identified Neurons Ruth L. Stornetta 10. Visualization of Activated Neurons Involved in Endocrine and Dietary Pathways Using GFP-Expressing Mice Rim Hassouna, Odile Viltart, Lucille Tallot, Karine Bouyer, Catherine Videau, Jacques Epelbaum, Virginie Tolle, and Emilio Badoer 11. Use and Visualization of Neuroanatomical Viral Transneuronal Tracers J. Patrick Card and Lynn W. Enquist 12. Visualization of Thermal Changes in Freely Moving Animals Daniel M.L. Vianna and Pascal Carrive 13. Perfusion Magnetic Resonance Imaging Quantification in the Brain Fernando Calamante Audrey Bonnan, Benjamin Grewe, and Andreas Frick 9. Juxtacellular Labeling in Combination with Other Histological Techniques to Determine Phenotype of Physiologically Identified Neurons Ruth L. Stornetta 10. Visualization of Activated Neurons Involved in Endocrine and Dietary Pathways Using GFP-Expressing Mice Rim Hassouna, Odile Viltart, Lucille Tallot, Karine Bouyer, Catherine Videau, Jacques Epelbaum, Virginie Tolle, and Emilio Badoer 11. Use and Visualization of Neuroanatomical Viral Transneuronal Tracers J. Patrick Card and Lynn W. Enquist 12. Visualization of Thermal Changes in Freely Moving Animals Daniel M.L. Vianna and Pascal Carrive 13. Perfusion Magnetic Resonance Imaging Quantification in the Brain Fernando Calamante Ruth L. Stornetta 10. Visualization of Activated Neurons Involved in Endocrine and Dietary Pathways Using GFP-Expressing Mice Rim Hassouna, Odile Viltart, Lucille Tallot, Karine Bouyer, Catherine Videau, Jacques Epelbaum, Virginie Tolle, and Emilio Badoer 11. Use and Visualization of Neuroanatomical Viral Transneuronal Tracers J. Patrick Card and Lynn W. Enquist 12. Visualization of Thermal Changes in Freely Moving Animals Daniel M.L. Vianna and Pascal Carrive 13. Perfusion Magnetic Resonance Imaging Quantification in the Brain Fernando Calamante 10. Visualization of Activated Neurons Involved in Endocrine and Dietary Pathways Using GFP-Expressing Mice Rim Hassouna, Odile Viltart, Lucille Tallot, Karine Bouyer, Catherine Videau, Jacques Epelbaum, Virginie Tolle, and Emilio Badoer 11. Use and Visualization of Neuroanatomical Viral Transneuronal Tracers J. Patrick Card and Lynn W. Enquist 12. Visualization of Thermal Changes in Freely Moving Animals Daniel M.L. Vianna and Pascal Carrive 13. Perfusion Magnetic Resonance Imaging Quantification in the Brain Fernando Calamante Rim Hassouna, Odile Viltart, Lucille Tallot, Karine Bouyer, Catherine Videau, Jacques Epelbaum, Virginie Tolle, and Emilio Badoer 11. Use and Visualization of Neuroanatomical Viral Transneuronal Tracers J. Patrick Card and Lynn W. Enquist 12. Visualization of Thermal Changes in Freely Moving Animals Daniel M.L. Vianna and Pascal Carrive 13. Perfusion Magnetic Resonance Imaging Quantification in the Brain Fernando Calamante J. Patrick Card and Lynn W. Enquist 12. Visualization of Thermal Changes in Freely Moving Animals Daniel M.L. Vianna and Pascal Carrive 13. Perfusion Magnetic Resonance Imaging Quantification in the Brain Fernando Calamante Daniel M.L. Vianna and Pascal Carrive 13. Perfusion Magnetic Resonance Imaging Quantification in the Brain Fernando Calamante 13. Perfusion Magnetic Resonance Imaging Quantification in the Brain Fernando Calamante Fernando Calamante
