Description
In Neuronal Cell Culture: Methods and Protocols, the latest aspects of the culture of neural cells are explored by experts in the field who also explain the practical and theoretical considerations of the techniques involved. Starting with a general overview of the neuronal culturing principles that are described, this detailed volume covers cell line models for neural cells, the isolation and propagation of primary cultures, stem cells, transfection and transduction of neural cultures, and other more advanced techniques. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and easy to use, Neuronal Cell Culture: Methods and Protocols will be of interest to scientists at all levels developing cell culture models for neuroscientific studies. 1. General Overview of Neuronal Cell Culture Jennifer Gordon, Shohreh Amini, and Martyn K. White 2. Considerations for the Use of SH-SY5Y Neuroblastoma Cells in Neurobiology Jane Kovalevich and Dianne Langford 3. Cultured Cell Line Models of Neuronal Differentiation: NT2, PC12 Nune Darbinian 4. Murine Teratocarcinoma-Derived Neuronal Cultures Prasun K. Datta 5. Isolation and Propagation of Primary Human and Rodent Embryonic Neural Progenitor Cells and Cortical Neurons Armine Darbinyan, Rafal Kaminski, Martyn K. White, Nune Darbinian, and Kamel Khalili 6. Mouse Enteric Neuronal Cell Culture Yonggang Zhang and Wenhui Hu 7. Preparation of Neural Stem Cells and Progenitors: Neuronal Production and Grafting Applications Joseph F. Bonner, Christopher J. Haas, and Itzhak Fischer 8. Derivation of Neuronal Cells from Fetal Normal Human Astrocyte (NHA) S. Ausim Azizi and Barbara Krynska 9. Slice Culture Modeling of Central Nervous System (CNS) Viral Infection Kalen R. Dionne and Kenneth L. Tyler 10. Neurospheres and Glial Cell Cultures: Immunocytochemistry for Cell Phenotyping Amanda Parker Struckhoff and Luis Del Valle 11. Transfection of Neuronal Cultures Ilker Kudret Sariyer 12. Lentiviral Transduction of Neuronal Cells Hassen S. Wollebo, Baheru Woldemichaele, and Martyn K. White 13. Compartmentalized Neuronal Cultures Armine Darbinyan, Paul Pozniak, Nune Darbinian, Martyn K. White, and Kamel Khalili 14. Quantitative Assessment of Neurite Outgrowth Over Growth Promoting or Inhibitory Substrates George M. Smith, Yingpeng Liu, and Jee W. Hong
