Description
This volume provides comprehensive information and detailed laboratory protocols used in the study of prion diseases. The chapters in this book cover topics such as: electron microscopy, neuropathology, immunohistochemistry, and immune-gold electron microscopy of prion diseases. Chapters also provide readers with detailed information about kuru, the clinical description of prion diseases, and the detection of prion protein and biomarkers. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and authoritative, Prion Diseases is a valuable resource for researchers and scientists in the exciting and rapidly growing field of prion disease research. Series Preface… Dedication… Preface… Table of Contents… Contributing Authors… 1. Kuru: Introduction to Prion Diseases Pawel P. Liberski, Agata Gajos, and Beata Sikorska 2. Anthropological Methods used in Kuru Research Shirley Lindenbaum 3. Nonhuman Primates in Research on Transmissible Spongiform Encephalopathies David M. Asher, Pedro Piccardo, and Luisa Gregori 4. Clinical Features and Diagnosis of Human Prion Diseases Richard Knight 5. Neuropathology, Immunohistochemistry, and Biochemistry in Human Prion DiseasesDiane L. Ritchie and James W. Ironside 6. Sub-Cellular and Molecular Changes Associated with Abnormal PrP Accumulation in Brain and Viscera of Classical a nd Atypical Prion Diseases Martin Jeffrey and Gillian McGovern 7. Electron Microscopy of Prion Diseases Pawel P. Liberski 8. Cell Death and Autophagy in Prion Diseases Pawel P. Liberski 9. Methods for Isolation of Spiroplasma sp. from Prion Positive Eye Tissues of Sheep Affected with Terminal Scrapie Frank O. Bastian 10. Detection and Diagnosis of Prion Diseases using RT-QuIC: An Update Byron Caughey, Christina D. Orru, Bradley R. Groveman, Matilde Bongianni, Andrew G. Hughson, Lynne D. Raymond, Matteo Manca, Allison Kraus, Gregory J. Raymond, Michele Fiorini, Maurizio Pocchiari, and Gianluigi Zanusso 11. Analysis of Charge Isoforms of the Scrapie Prion Protein using Two-Dimensional Electrophoresis Elizaveta Katorcha and Ilia V. Baskakov 12. Exosome s in Prion Diseases Alexander Hartmann, Hermann Altmeppen, Susanne Krasemann, and Markus Glatzel 13. Synthetic Mammalian Prions Fabio Moda, Edoardo Bistaffa, Joanna Narkiewicz, Giulia Salzano, and Giuseppe Legname 14. Biomarkers in Cerebrospinal Fluid Joanna Gawinecka, Matthias Schmitz, and Inga Zerr 15. The Use of Transgenic and Knock Out Mice in Prion Research Abigail B. Diack and Jean C. Manson 16. Transgenic Mouse Models of Prion Diseases Julie Moreno and Glenn C. Telling
