Description
Preface… Table of Contents… Contributing Authors… 1. Single-Cell RNA Sequencing of Oligodendrocyte Lineage Cells from the Mouse Central Nervous System Sueli Marques, David van Bruggen, and Gonalo Castelo-Branco 2. Isolation and Preparation of Cells from Focal Remyelinating Central Nervous System Lesion sfor RNA Sequencing Claire L. Davies, Stefka Gyoneva, Anne Cotleur, Richard M. Ransohoff, and Veronique E. Miron 3. Myelin: Methods for Purification and Proteome Analysis Michelle S. Erwig, Drte Hesse, Ramona B. Jung, Marina Uecker, Kathrin Kusch, Stefan Tenzer, Olaf Jahn, and Hauke B. Werner 4. In Vitro Generation and Electrophysiological Characterization of OPCc and Oligodendrocytes from Human Pluripotent Stem Cells Dario Magnani, Siddharthan Chandran, David J.A. Wyllie, and Matthew R. Livesey 5. Isolation and Culture of Oligodendrocytes Marie-Theres Weil, Giselheid Schulz-berlin, Chaitali Mukherjee, Wen Ping Kuo-Elsner, Isabelle Schfer, Christina Mller, and Mikael Simons 6. A Neuron-Free Microfiber Assay to Assess Myelin Sheath Formation Marie E. Bechler 7. Oligodendrocyte-Neuron Myelinating Co-Culture Matthew Swire and Chalres ffrench-Constant 8. Teasing of Ventral Spinal Cord White Matter Fibres for the Analysis of Central Nervous System Nodes of Ranvier Andrew A. Jarjour and Diane L. Sherman 9. Whole-Cell Patch Clamp Recordings from Oligodendrocyte Lineage Cells in Brain Slices Sylvia Agathou and Ragnhildur Thra Kradttir 10. Ex Vivo Slice Cultures to Study Myelination, Demyelination, and Remyelination in Mouse Brain and Spinal Cord Sowmya Sekiza and Anna Williams 11. Forward Genetic Screen using Zebrafish to Identify New Genes Involved in Myelination Linde Kegel, Maria Rubio, Rafael Almeida, Silvia Benito, Anna Klingseisen, and David A. Lyons 12. Manipulating Neuronal Activity in the Developing Zebrafish Spinal Cord to Investigate Adaptive Myelination Jill M. Williamson, David A. Lyons, and Rafael G. Almeida 13. A Drug Inducible Transgenic Zebrafish Model for Myelinating Glial Cell Ablation Marja J. Karttunen and David A. Lyons 14. Conditional Demyelination and Remyelination in a Transgenic Xenopus laevis Abdelkrim Mannioui and Barnard zalc 15. Conditional Mutagenesis in Oligodendrocyte Lineage Cells Sandra Goebbels and Klaus-Armin Nave 16. Recent Advances in Live Imaging of Cells of the Oligodendrocyte Lineage Jaime Eugenin-von Bernhardi and Leda Dimou 17. The DTA Mouse Model for Oligodentrocyte Ablation and CNS Demyelination Maria Traka 18. Human Glial Chimeric Mice to Define the Role of Glial Pathology in Human Disease John N. Mariani, Lisa Zou, and Steven A. Goldman 19. A Method to Detect Cytochrome c Oxidase Activity and Mitochondrial Proteins in Oligodencrocytes Graham R. Campbell and Don J. Mahad 20. Transmission Electron Microscopy of Oligodendrocytes and Myelin Marie-Theres Weil, Torben Ruhwedel, Martin Meschkat, Mogusia Sadowski, and Wiebke Mbius 21. Toxin-Based Models to Investigate Demyelination and Remyelination Christopher E. McMurran, Chao Zhao, and Robin J M. Franklin 22. Magnetic Resonance techniques for Imaging White Matter Cassandra Sampaio-Baptista, Kata Diosi, and Heidi Johansen-Berg
