Description
This volume discusses the latest analytical approaches used to sample defined molecular populations of metabolites via functional group derivatization, specialized chromatographic methods, and ionization techniques. Chapters cover key methods for sample introductions to the ion source, including direct flow, gas chromatography, liquid chromatography, and capillary electrophoresis. Chapters also explore non-targeted and targeted analyses, as well as the emerging field of metallomics. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and authoritative, Metabolomics is a valuable resource for students, researchers, practicing physicians and veterinarians, and administrators involved in the funding of research. Series Preface… Preface… Table of Contents… Contributing Authors… 1. Flow Infusion ESI High Resolution Mass Spectrometry Metabolomics Analytical Platforms Paul L. Wood 2. High Throughput Metabolomics using Flow Injection Analysis and Fourier Transform Ion Cyclotron Resonance-Mass Spectrometry Estelle Rathahao-Paris, Sandra Alves, and Alain Paris 3. Two-Dimensional Liquid Chromatography in Metabolomics and Lipidomics Miriam C. Prez-Cova, Rom Tauler, and Joaquim Jaumot 4. Metabolomics of Small Numbers of Cells using Chemical Isotope Labeling Combined with Nanoflow LC-MS Xian Luo and Liang Li 5. Untargeted Metabolomics to Interpret the Effects of Social Defeat: Integrating Chemistry and Behavioral Approaches in Neuroscience Allen K. Bourdon and Brooke N. Dulka 6. Flow Infusion Electrospray Ionization High Resolution Mass Spectrometry of the Chloride Adducts of Sphingolipids, Glycerophospholipids, Glycerols, Hydroxy Fatty Acids, Fatty Acid Esters of Hydroxy Fatty Acids (FAHFA), and Modified Nucleosides Paul L. Wood and Randall L. Woltjer 7. High Mass Accuracy Gas Chromatography/Mass Spectrometry for Performing Isotopic Ratio Outlier Analysis: Applications for Non-Annotated Metabolite Detection Yunping Qiu and Irwin J. Kurland 8. Enantioseparation and Detection of (R)-2-hydroxyglutarate and (S)-2-hydroxyglutarate by Chiral Gas Chromatography-Triple Quadrupole Mass Spectrometry Shinji K. Strain, Morris D. Groves, and Mark R. Emmett 9. Enantioselective Supercritical Fluid Chromatography (SFC) for Chiral Metabolomics Robert Hofstetter, Andreas Link, and Georg M. Fassauer 10. Capillary Electrophoresis-Mass Spectrometry of Hydrophilic Metabolomics Masahiro Sugimoto 11. Lipidomic Analysis of Oxygenated Polyunsaturated Fatty Acid-Derived-Inflammatory Mediators in Neurodegenerative Diseases Mauricio Mastrogiovannim Estefana Ifrn, Andrs Trostchansky, and Homero Rubbo 12. The Characterization and Quantification of the Fatty Acid Amidome Kristen A. Jeffries, Emma K. Farrell, Ryan L. Anderson, Gabriela Suarez, Amanda J. Goyette Osborne, McKenzi K. Heide, and David J. Merkler 13. Metabolomics Analysis of Complex Biological Specimens using Nuclear Magnetic Resonance Spectroscopy Khushboo Gulati, Sharanya Sarkar, and Krishna Mohan Poluri 14. Untargeted Metabolomics Methods to Analyze Blood Derived Samples Danuta Dudzik and Antonia Garca 15. Multi-Compartmental High-Throughput Metabolomics Based on Mass Spectrometry Ral Gonzlez-Domnguez, lvaro Gonzlez-Domnguez, Ana Sayago, and ngeles Fernndez-Recamales 16. GC-MS Non-Targeted Metabolomics of Neural Tissue Carolina Gonzalez-Riano, M Fernanda Rey-Stolle, and Antonia Garca 17. GC-MS of Pentafluourobenzyl Derivatives of Phenols, Amines, Carboxylic Acids Paul L. Wood 18. GC-MS of tert-Butyldimethylsilyl (tBDMS) Derivatives of Neurochemicals Paul L. Wood 19. UHPLC-MS/MS Method for Determination of Biologically Important Thiols in Plasma using New Derivatizing Maleimide Reagent Dominika Olesova and Andrej Kovac 20. Untargeted Metabolomics Determination of Postmortem Changes in Brain Tissue Samples by UHPLC-ESI-QTOF-MS and GC-EI-Q-MS Carolina Gonzalez-Riano, Antonia Garca, and Coral Barbas 21. Metallomics Imaging Valderi Luiz Dressler, Graciela Marini Hiedrich, Vinicius Machado Neves, Eson Irineu Mller, and Dirce Pozebon Subject Index List…
