Description
This second edition volume details the latest aspects of neural cells covering the practical and theoretical considerations of each techniques involved. Chapters guide readers through a general overview of the neuronal culturing principles, cell line models for neural cells, the isolation and propagation of primary cultures, stem cells, transfection and transduction of neural cultures, and other more advanced techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and easy to use, Neuronal Cell Culture: Methods and Protocols, Second Edition aims to be of interest to scientists at all levels studying cell culture models for neuroscientific studies. 1. 1. General Overview of Neuronal Cell Culture Jennifer Gordon and Shohreh Amini 2. 2. Considerations for the use of SH-SY5Y Neuroblastoma Cells in Neurobiology Jane Kovalevich, Maryline Santerre, and Dianne Langford 3. 3. Cultured cell line models of neuronal differentiation: NT2, PC12, SK-N-MC Nune Darbinian 4. 4. Murine teratocarcinoma-derived neuronal cultures Prasun K. Datta 5. 5. Isolation and propagation of primary human and rodent embryonic neural progenitor cells and cortical neurons Armine Darbinyan, Rafal Kaminski, Martyn K White, Paul D Pozniak, Nune Darbinian, and Kamel Khalili 6. 6. Mouse Enteric Neuronal Cell Culture6 Yonggang Zhang and Wenhui Hu 7. 7. Preparation of Neural Stem Cells and Progenitors: Neuronal Production and Grafting Applications Lyandysha V. Zholudeva, Ying Jin, Liang Qiang, Michael A. Lane, and Itzhak Fischer 8. 8. Slice culture modeling of CNS viral infection Kalen R. Dionne, Kenneth L. Tyler, and Penny Clarke 9. 9. Neurospheres and Glial cell cultures: immunocytochemistry for cell phenotyping Amanda Parker Struckhoff and Luis Del Valle 10. 10. Transfection of Neuronal Cultures Ilker Kudret Sariyer 11. 11. Lentiviral Transduction of Neuronal Cells Anna Bellizzi, Nicholas Ahye, and Hassen S. Wollebo 12. 12. Compartmentalized Neuronal Cultures Armine Darbinyan, Paul Pozniak, Nune Darbinian, Martyn K. White, and Kamel Khalili 13. 13.Quantitative assessment of neurite outgrowth over growth promoting or inhibitory substrates George M. Smith and Yingpeng Liu 14. 14. Isolation and Culture of Dorsal Root Ganglia (DRG) from Rodents Masoud Shekarabi, Jake A. Robinson, and Tricia H. Burdo 15. 15. Isolation of Primary Human and Rodent Brain Microvascular Endothelial Cells: Culturing, Characterization, and High-Efficiency Transfection Nune Darbinian, Armine Darbinyan, Nana Merabova, and Shohreh Amini 16. 16..Fetal Brain Injury Models of Fetal Alcohol Syndrome: Examination of Neuronal Morphologic Condition Using Sholl Assay Nune Darbinian, Armine Darbinyan, Kamel Khalili, and Shohreh Amini
