Description
Part I: Biochemical Experiments and Cellular Models of Parkinson’s Disease 1. a-Synuclein Seeding Assay Using RT-QuIC Ayami Okuzumi, Taku Hatano, Takeshi Fukuhara, Shinichi Ueno, Nobuyuki Nukina, Yuzuru Imai, and Nobutaka Hattori 2. Electron-Microscopic Analysis of a-Synuclein Fibrils Airi Tarutani and Masato Hasegawa 3. a-Synuclein Seeding Assay Using Cultured Cells Jun Ogata, Daisaku Takemoto, Shotaro Shimonaka, Yuzuru Imai, and Nobutaka Hattori 4. Analysis of a-Synuclein in Exosomes Taiji Tsunemi, Yuta Ishiguro, Asako Yoroisaka, and Nobutaka Hattori 5. Measurement of GCase Activity in Cultured Cells Yuri Shojima, Jun Ogata, Taiji Tsunemi, Yuzuru Imai, and Nobutaka Hattori 6. Detection of Substrate Phosphorylation of LRRK2 in Tissues and Cultured Cells Kyohei Ito, Lejia Xu, Genta Ito, and Taisuke Tomita 7. Two Methods to Analyze LRRK2 Functions Under Lysosomal Stress: The Measurements of Cathepsin Release and Lysosomal Enlargement Maria Sakurai and Tomoki Kuwahara 8. Differentiation of Midbrain Dopaminergic Neurons from Human iPS Cells Kei-ichi Ishikawa, Risa Nonaka, and Wado Akamatsu 9. Monitoring PINK1-Parkin Signaling Using Dopaminergic Neurons from iPS Cells Kahori Shiba-Fukushima and Yuzuru Imai Part II: Mammalian Models of Parkinson’s Disease 10. Generation of Mitochondrial Toxin Rodent Models of Parkinson’s Disease Using 6-OHDA, MPTP, and Rotenone Hiroharu Maegawa and Hitoshi Niwa 11. Midbrain Slice Culture as an Ex Vivo Analysis Platform for Parkinson’s Disease Yuji Kamikubo, Keiko Wakisaka, Yuzuru Imai, and Takashi Sakurai 12. a-Synuclein Propagation Mouse Models of Parkinson’s Disease Norihito Uemura, Jun Ueda, Shinya Okuda, Masanori Sawamura, and Ryosuke Takahashi 13. Common Marmoset Model of a-Synuclein Propagation Masami Masuda-Suzukake, Aki Shimozawa, Masashi Hashimoto, and Masato Hasegawa 14. Application of a Tissue Clearing Method for the Analysis of Dopaminergic Axonal Projections Kenta Yamauchi, Megumu Takahashi, and Hiroyuki Hioki 15. Deep Brain Stimulation Using Animal Models of Parkinson’s Disease Asuka Nakajima and Yasushi Shimo Part III: Invertebrate Models of Parkinson’s Disease 16. Assessment of Cytotoxicity of a-Synuclein in Budding Yeast Using a Spot Growth Assay and Fluorescent Microscopy Masak Takaine 17. The Functional Assessment of LRRK2 in Caenorhabditis elegans Mechanosensory Neurons Tomoki Kuwahara 18. Analysis of Dopaminergic Functions in Drosophila Tsuyoshi Inoshita, Daisaku Takemoto, and Yuzuru Imai 19. Evaluation of Mitochondrial Function and Morphology in Drosophila Yinglu Tang, Foozhan Tahmasebinia, and Zhihao Wu 20. Cytosolic and Mitochondrial Ca2+ Imaging in Drosophila Dopaminergic Neurons Tsuyoshi Inoshita and Yuzuru Imai
