Description
1. Purification of MAO A and of MAO B from Mammalian Tissue Sources Dale E. Edmondson 2. Purification of Recombinant Eukaryotic MAO A and MAO B Utilizing the Pichia pastoris Expression System Dale E. Edmondson 3. The Peroxidase-Coupled Assay to Measure MAO Enzymatic Activity Joana Reis and Claudia Binda 4. Measurement of MAO Enzymatic Activity by Spectrophotometric Direct Assays Joana Reis and Claudia Binda 5. Radiochemical Assay of Monoamine Oxidase Activity Andrew Holt 6. MAO Visible Spectroscopy for Ligand Interactions, Redox Chemistry, and Kinetics of Irreversible Inhibition Rona R. Ramsay 7. Conventional Receptor Radioligand Binding Techniques Applied to the Study of Monoamine Oxidase Andrew Holt 8. Assay of MAO Inhibition by Chromatographic Techniques (HPLC/HPLC-MS) Toms Herraiz 9. Crystallization of Human Monoamine Oxidase B Claudia Binda, Dale E. Edmondson, and Andrea Mattevi 10. Detecting Monoamine Oxidase A and B Proteins: A Western Blotting Protocol and Some Practical Considerations Jennifer N.K. Nyarko, Ryan M. Heistad, Paul R. Pennington, and Darrell D. Mousseau 11. An (Immune)Fluorescence Protocol for Monitoring Monoamine Oxidase A/B Protein Distribution within the Cell Tyler J. Wenzel, Jennifer N.K. Nyarko, Ryan M. Heistad, Paul R. Pennington, Chris P. Phenix, and Darrell D. Mousseau 12. Expression and Function of MAO A in Cardiac Cells by Means of Adenovirus-Mediated Gene-Transfer Yohan Santin, Angelo Parini, and Jeanne Mialet-Perez 13. In Vitro and In Vivo Assays Characterizing MAO A Function in Cancers Boyang Jason Wu and Jean C. Shih 14. In Vivo Study of Monoamine Oxidases Using Multisite Intracerebral Microdialysis Philippe De Deurwaerdre, Nouhad Samb, Hasna El Boukhari, Rmi Corne, Abdeslam Chagraoui, and Giuseppe Di Giovanni 15. Informed Use of 3D-QSAR for the Rational Design of Coumarin Derivatives as Potent and Selective MAO B Inhibitors Nicola Gambacorta, Marco Catto, Leonardo Pisani, Angelo Carotti, and Orazio Nicolotti 16. Hansch-Type QSAR Models for the Rational Design of MAO Inhibitors: Basic Principles and Methodology Leonardo Pisani, Modesto de Candia, Mariagrazia Rullo, and Cosimo D. Altomare 17. Computational Chemistry and Molecular Modeling of Reversible MAO Inhibitors Kemal Yeleki and Safiye Sag Erdem
