Description
Therapeutic Peptides: Methods and Protocols features biological methods for the preparation of peptide phage display libraries using both filamentous and lytic phage. With contributions from renowned authors in the field, the book also explores selection and screening of the prepared peptide libraries for peptides with the desired function and the subsequent characterization of the identified peptides. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and dependable, Therapeutic Peptides: Methods and Protocols is an ideal guide for researchers from all backgrounds seeking methods for the identification of therapeutic peptide candidates. 1. Design of Phage-Displayed Cystine-Stabilized Mini-Protein Libraries for Proteinaceous Binder Engineering Hung-Ju Chang and An-Suei Yang 2. Construction of a Filamentous Phage Display Peptide Library Annette Fagerlund, Astrid Hilde Myrset, and Mari Ann Kulseth 3. Engineering Bio-Active Peptide-Based Therapeutic Molecules Jong Sang Ryu, A. Yeon Cho, Sang Won Seo, and Hosung Min 4. T7 Lytic Phage-Displayed Peptide Libraries: Construction and Diversity Characterization Lauren R.H. Krumpe and Toshiyuki Mori 5. Affinity Selection Using Filamentous Phage Display Mari Ann Kulseth, Annette Fagerlund, and Astrid Hilde Myrset 6. Bioprospecting Open Reading Frames for Peptide Effectors Ling Xiong and Charles Scott 7. Identification of Ideal Peptides for Heterovalent Ligands Ajay Shrivastava, Matthew von Wronski, Michael F. Tweedle, and Adrian D. Nunn 8. Substrate Phage Display for Protease Substrate Sequence Characterization: Bovine Factor Xa as a Model System Hung-Ju Hsu and An-Suei Yang 9. Engineering Peptide Therapeutics Using MIMETIBODY(TM) Technology Kristen Picha, Chichi Huang, Peter Bugelski, and Karyn O’Neil 10. Evaluation of Peptides as Protease Inhibitors and Stimulators Hannu Koistinen, Can Hekim, Ping Wu, Ale Nrvnen, and Ulf-Hkan Stenman 11. Assessment of Antimicrobial (Host Defense) Peptides as Anti-Cancer Agents Susan Douglas, David Hoskin, and Ashley Hilchie 12. Peptide Labelling Strategies for Imaging Agents Tuulia Huhtala, Janne Weisell, Jussi Rytknen, and Ale Nrvnen 13. Peptide Optimization and Conjugation Strategies in the Development of Molecularly Targeted Magnetic Resonance Imaging Contrast Agents Andrew F. Kolodziej, Zhaoda Zhang, Kirsten Overoye-Chan, Vincent Jacques, and Peter Caravan 14. Evaluation of Prenylated Peptides for Use in Cellular Imaging and Biochemical Analysis Joshua D. Ochocki, Urule Igbavboa, W. Gibson Wood, Elizabeth V. Wattenberg, and Mark D. Distefano 15. Ultraviolet Absorption Spectroscopy of Peptides Mangala R. Liyanage, Kunal Bakshi, David B. Volkin, and C. Russell Middaugh 16. Fluorescence Spectroscopy of Peptides Mangala R. Liyanage, Kunal Bakshi, David B. Volkin, and C. Russell Middaugh 17. Circular Dichroism of Peptides Kunal Bakshi, Mangala R. Liyanage, David B. Volkin, and C. Russell Middaugh 18. Fourier Transform Infrared Spectroscopy of Peptides Kunal Bakshi, Mangala R. Liyanage, David B. Volkin, and C. Russell Middaugh
