Description
With genetic engineering, systems explored in this book now exist allowing for the simple, efficient, and near universally precise genetic manipulation directly in any organism, including the mouse. Herein, these models are applied to a wide field of disease areas, including diabetes, cardiovascular disease, skin disorders, cancer, neurodegenerative and neuromuscular diseases, retinal disorders, as well as various behavioral models. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and fully updated, Mouse Models for Drug Discovery: Methods and Protocols, Second Edition serves to equip the reader with an extensive overview of techniques to utilize the many possibilities of mice in the drug development process. 1. Genetically Defined Strains in Drug Development and Toxicity Testing Michael F.W. Festing 2. Simple, Efficient CRISPR-Cas9 Mediated Gene Editing in Mice: Strategies and Methods Benjamin E. Low, Peter M. Kutny, and Michael V. Wiles 3. Mouse Sperm Cryopreservation and Recovery of Genetically Modified Mice Benjamin E. Low, Rob A. Taft, and Michael V. Wiles 4. Generation of Immunodeficient Mice Bearing Human Immune Systems by the Engraftment of Hematopoietic Stem Cells Suheyla Hasgur, Ken Edwin Aryee, Leonard D. Shultz, Dale L. Greiner, and Michael A. Brehm 5. Generation of Human Liver Chimeric Mice for the Study of Human Hepatotropic Pathogens Markus von Schaewen, Gabriela Hrebikova, and Alexander Ploss 6. Human FcRn Transgenic Mice for Pharmacokinetic Evaluation of Therapeutic Antibodies Derry C. Roopenian, Gregory J. Christianson, Gabriele Proetzel, and Thomas J. Sproule 7. A Humanized Mouse Model to Study Human Albumin and Albumin Conjugates Pharmacokinetics Benjamin E. Low and Michael V. Wiles 8. Germ-Free Mice Model for Studying Host-Microbial Interactions Yogesh Bhattarai and Purna Kashyap 9. Bridging Mice to Men: Using HLA Transgenic Mice to Enhance the Future Prediction and Prevention of Autoimmune Type 1 Diabetes in Humans David V. Serreze, Marijke Niens, John Kulik, and Teresa P. DiLorenzo 10. Mouse Models of Type 2 Diabetes Mellitus in Drug Discovery Helene Baribault 11. Cholesterol Absorption and Metabolism Philip N. Howles 12. Skin Diseases in Laboratory Mice: Approaches to Drug Target Identification and Efficacy Screening John P. Sundberg, Kathleen A. Silva, Lloyd E. King, Jr, and C. Herbert Pratt 13. Chronic Myeloid Leukemia (CML) Mouse Model in Translational Research Cong Peng and Shaoguang Li 14. Murine Model for Colitis-Associated Cancer of the Colon Ashley J. Snider, Agnieszka B. Bialkowska, Amr M. Ghaleb, Vincent W. Yang, Lina M. Obeid, and Yusuf A. Hannun 15. Mouse Models for Studying Depression-Like States and Antidepressant Drugs Carisa L. Bergner, Amanda N. Smolinsky, Peter C. Hart, Brett D. Dufour, Rupert J. Egan, Justin L. LaPorte, and Allan V. Kalueff 16. Experimental Models of Anxiety for Drug Discovery and Brain Research Peter C. Hart, Carisa L. Bergner, Amanda N. Smolinsky, Brett D. Dufour, Rupert J. Egan, Justin L. LaPorte, and Allan V. Kalueff Stacey J. Sukoff Rizzo 18. Better Utilization of Mouse Models of Neurodegenerative Diseases in Preclinical Studies: From the Bench to the Clinic Christopher Janus, Carolina Hernandez, Victoria deLelys, Hanno Roder, and Hans Welzl 19. Neuromuscular Disease Models and Analysis Robert W. Burgess, Gregory A. Cox, and Kevin L. Seburn 20. Brightfield Imaging and Optical Coherence Tomography of the Mouse Posterior Eye Mark P. Krebs, Mei Xiao, Keith Sheppard, Wanda Hicks, and Patsy M. Nishina 21. Mouse Models as Tools to Identify Genetic Pathways for Retinal Degeneration, as Exemplified by Leber’s Congenital Amaurosis Bo Chang
