Description
G Protein-Coupled Receptor Genetics: Research and Methods in the Post-Genomic Era features practical techniques inspired by the fast moving GPCR field. From powerful bioinformatic tools tracing the evolution of GPCRs, to methods for the cellular transfection of engineered viruses containing GPCRs, to optogenetic techniques that produce light-activated GPCRs in live mice, what was once science fiction is now science fact. This detailed volume includes sections covering genetic mechanisms, a genetic toolbox for GPCR discovery, as well as genetic aspects of G protein-coupled receptors in health and medicine. Written for the Methods in Pharmacology and Toxicology series, this book contains the kind of key implementation advice that encourages successful results in the lab. Authoritative and easy to use, G Protein-Coupled Receptor Genetics: Research and Methods in the Post-Genomic Era serves as an ideal guide for researchers aiming to continue our progress in this dynamic and exciting area of study. Part I: Genetic Mechanisms of G Protein-Coupled Receptors 1. G Protein-Coupled Receptors: Research and Methods in the Post-Genomic Era Craig W. Stevens 2. Examining the Dynamic Evolution of G Protein-Coupled Receptors Claudia Stubert, Diana Le Duc, and Torsten Schneberg 3. Functional Properties of Virus-Encoded and Virus-Regulated G Protein-Coupled Receptors Katja Spiess and Mette M. Rosenkilde 4. Discovery and Function of the Very Large G Protein-Coupled Receptor Perrin C. White and D. Randy McMillan 5. Functional Evolution of Opioid Family G Protein-Coupled Receptors Eyal Vardy, Craig W. Stevens, and Bryan L. Roth 6. Investigation of G Protein-Coupled Receptor Function and Regulation Using Antisense Jonathon Mark Willets and Craig Nash 7. Signalosome Profiling Reveals Allosteric Interactions Between G Protein-Coupled Receptors Katrin Altosaar, Darlaine Ptrin, Dominic Devost, and Terence E. Hbert Part II: Genetic Toolbox for G Protein-Coupled Receptor Discovery 8. Use of Optogenetic Approaches to Control Intracellular Signaling of G Protein-Coupled Receptors Olivia A. Masseck, Melanie D. Mark, and Stefan Herlitze 9. Viral Infection for G Protein-Coupled Receptor Expression in Eukaryotic Cells Antonio Porcellini, Luisa Iacovelli, and Antonio De Blasi 10. Using In Vitro Mutagenesis to Characterize Structure-Function Relationships in G Protein-Coupled Receptors Gregory W. Sawyer and Frederick J. Ehlert 11. The CRE Luc Mouse Model for Bioimaging Ligand Activation of G Protein-Coupled Receptors H. Gregory Polites and Kyriakos D. Economides 12. Inducing Conformational Changes in G Protein-Coupled Receptors by Domain Coupling Hamiyet Unal and Sadashiva S. Karnik 13. Rescue of Defective G Protein-Coupled Receptor Function by Intermolecular Cooperation Ashutosh Trehan, Kim Carol Jonas, Ilpo Huhtaniemi, Aylin Carla Hanyaloglu, and Adolfo Rivero-Mller 14. Design of Super-Arrestins for Gene Therapy of Diseases Associated with Excessive Signaling of G Protein-Coupled Receptors Vsevolod V. Gurevich and Eugenia V. Gurevich Part III: Genetic Aspects of G Protein-Coupled Receptors in Health and Medicine 15. Importance of G Protein-Coupled Receptor Genetics in Clinical Medicine Ramakrishna Devaki 16. Pharmacogenomics of G Protein-Coupled Receptor Signaling and Other Pathways in Essential Hypertension Pedro A. Jose, Van Anthony M. Villar, Matthew R. Weir, and Robin A. Felder 17. Exon Sequencing of G Protein-Coupled Receptor Genes and Perspectives for Disease Treatment Angeliki Karamitri and Ralf Jockers 18. Gene Therapy Using G Protein-Coupled Receptors for the Treatment of Cardiovascular Disease Dario Leosco, Grazia D. Femminella, Claudio de Lucia, and Giuseppe Rengo 19. Novel Mechanisms of G Protein-Coupled Receptor Oligomer and Ion Channel Interactions in Nociception Ajay S. Yekkirala 20. Discovery and Characterization of Leucine-Rich Repeat-Containing G Protein-Coupled Receptors Marie-Isabelle Garcia, Valeria Fernandez-Vallone, and Gilbert Vassart 21. The Role of G-Protein Coupled Receptors in Adult Neurogenesis Katie M. Collette, Van A. Doze, and Dianne M. Perez
