Description
Part I: transcriptional and post-transcriptional analysis and cellular detection of opioid receptors 1. Overview of Genetic Analysis of Human Opioid Receptors Santi M. Spampinato 2. Renilla luciferase Reporter Assay to Study 3’UTR-driven post-Transcriptional Regulation of OPRM1 Gabriele Vincelliand Andrea Bedini 3. Fluorescence Colocalization Analysis of Cellular Distribution of MOR-1 Vittoria Borgonetti and Nicoletta Galeotti 4. Monitoring Opioid Receptors Interaction in Living Cells by Bioluminescence Resonance Energy Transfer (BRET) Monica Baiula 5. Bioluminescence Resonance Energy Transfer (BRET) to Detect the Interactions Between Kappa Opioid Receptor and Non-visual Arrestins Andrea Bedini 6. Functionalization and Bioconjugation of Nanoruby for Long-term, Ultrasensitive Imaging of -Opioid Receptors Rashmi Pillai , Mark Connor , and Varun Sreenivasan 7. Immunohistochemical Analysis of Opioid Receptors in Peripheral Tissues Yvonne Schmidt and Halina Machelska 8. Real-Time Quantitative Reverse Transcription PCR for Detection of Opioid Receptors in Immune Cells Melih zgr Celik, Dominika Labuz, and Halina Machelska Part II: Analysis of signaling events modulated by opioid receptors 9. Quantitative Analysis of MOR-1 Internalization in Spinal Cord of Morphine-tolerant Mice Vittoria Borgonetti and Nicoletta Galeotti 10. GTPgS-autoradiography for Studies of Opioid Receptor Functionality Alfhild Grnbladh and Mathias Hallberg 11. Evaluating Opioid Mediated Adenylyl Cyclase Inhibition in Live Cells using a BRET-based Assay Preeti Manandhar, Shivani Sachdev and Marina Santiago 12. Patch Clamp Analysis of Opioid-Induced Kir3 Currents in Mouse Peripheral Sensory Neurons Following Nerve Injury Viola Seitz, Philip Sttzner, Dominika Labuz, and Halina Machelska 13. Opioid Modulation of Neuronal Iron and Potential Contributions to NeuroHIV Bradley Nash, Elena Irollo, Renato Brandimarti, and Olimpia Meucci 14. An In Vitro Assay to Study the Role of Opioids in Modulating Immune Cell Adhesion Monica Baiula 15. Assessing Cell Viability Effects of Opioids in Primary Cortical Cells from Rat Alfhild Grnbladh, Erik Nylander, Sofia Zelleroth, and Mathias Hallberg 16. The Quest for More Effective Analgesics with Reduced Abuse Liability and Fewer Adverse Effects: Promises, Pitfalls and Future Perspectives of Biased Agonists at Opioid Receptors Andrea Bedini Part III: Model systems to studying opioid receptor-mediated functions 17. Mechanical Nociception in Mice and Rats: Measurement with Automated von Frey Equipment Gabriele Campana and Roberto Rimondini 18. Evaluation of Murine Macrophage Cytokine Production after In Vivo Morphine Treatment Silvia Franchi, Mara Castelli, Sarah Moretti, Alberto Panerai, and Paola Sacerdote 19. Measurement of Macrophage Toll like Receptor 4 Expression After Morphine Treatment Mara Castelli, Alberto Panerai, Paola Sacerdote, and Silvia Franchi Part IV Behavioral effects mediated by opioid receptors 20. Conditioned Place Preference (CPP) in Rats: From Conditioning to Reinstatement Test Maria Scherma, Liana Fattore,Walter Fratta,and Paola Fadda 21. Analysis of Opioid-Seeking Behavior through the Intravenous Self-administration Reinstatement Model in Rats Liana Fattore, Paola Fadda, Mary Tresa Zanda, and Walter Fratta 22. Induction of a High Alcohol Consumption in Rats and Mice: Role of Opioid Receptors in Rats and Mice Roberto Rimondini and Gabriele Campana 23. Early Life Stress as a Probe to Study the Opioid System in Developing Rodents Stefano Loizzo and Alberto Loizzo 24. Evaluation of m–opioid System Functionality in Mouse Pups: Ultrasonic Vocalizations as an Index Of Infant Attachment Francesca R. D’Amato
