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Preface… Table of Contents… Contributing Authors… Part I Human Brain Preparations 1. Protocol for the Systematic Fixation, Circuit-Based Sampling, and Qualitative and Quantitative Neuropathological Analysis of Human Brain Tissue Caitlin S. Latimer, Erica J. Melief, Jeanelle Ariza-Torres, Kim Howard, Amanda R. Keen, Lisa M. Keene, Aimee M. Schantz, Trevor M. Sytsma, Angela M. Wilson, Thomas J. Grabowski, Martin Darvas, Kristen Dams O’Connor, Amber L. Nolan, Brian L. Edlow, Christine L. Mac Donald, and C. Dirk Keene 2. Extraction and Purification of Single Nuclei from Frozen Human Brain Tissue Carter R. Palmer and Jerold Chun 3. Isolation of Human Microglia from Neuropathologically Diagnosed Cases in the Single-Cell Era Lih-Fen Lue, Douglas G. Walker, Suet Theng Beh, and Thomas G. Beach 4. Microglia in Human Postmortem Brain Samples: Quantitative Ultrastructural Analysis of Scanning Electron Microscopy Images Marie-Kim St-Pierre, Eva simoncicov, Mical Carrier, and Marie-ve Tremblay 5. Three-Dimensional Imaging of Fibrinogen and Neurovascular Alterations in Alzheimer’s Disease Maria Merlini, Elif G. Sozmen, Keshav S. Subramanian, Alissa L. Nana, William W. Seeley, and Katerina Akassoglou Part II Human Neural Cellular Models 6. Human Induced Pluripotent Stem Cell (hiPSC)-Derived Neurons and Glia for the Elucidation of Pathogenic Mechanisms in Alzheimer’s Disease Jessica E. Young and Lawrence S.B. Goldstein 7. Modeling Alzheimer’s Disease using Human Brain Organoids Karina Karmirian, Mariana Holubiec, Livia Goto-Silva, Ivn Fernndez Bessone, Gabriela Vitria, Beatriz Mello, Matias Alloatti, Bart Vanderborght, Toms L. Falzone, and Stevens Rehen 8. 3D Biohybrid StarPEG-Heparin Hydrogel Cultures for Modeling Human Neuronal Development and Alzheimer’s Disease Pathology Tohid Siddiqui, Hilal Celikkaya, Zeynep Tansu Atasavum, Stanislava Popova, Uwe Freudenberg, Carsten Werner, and Caghan Kizil Part III Nucleic Acid Analyses of Human Brain 9. Computational Approaches to Assess Abnormal Metabolism in Alzheimer’s Disease Using Transcriptomics Hatice Bsra Lleci, Dilara Uzuner, Tunahan akir, and Madhav Thambisetty 10. FISHing for Chromosome Instability and Aneuploidy in the Alzheimer’s Disease Brain Yuri B. Yurov, Svetlana G. Vorsanova, and Ivan Y. Iourov 11. Somatic CNV Detection by Single-Cell Whole-Genome Sequencing in Post-Mortem Human Brain Diego Perez-Rodriguez, Maria Kalyva, Catherine Santucci, and Christos Proukakis Part IV Lipids Analyses of Human Brain 12. Mass Spectrometry Analysis of the Human Brain Sphingolipidome Xin Ying Chua, Ryan Huang, Deron Herr, Mitchell K.P. Lai, Markus R. Wenk, and Federico Torta 13. Mass Spectrometry for the Advancement of Lipid Analysis in Alzheimer’s Research Jonatan Martnez-Gardeazabal, Marta Moreno-Rodrguez, Estbaliz Gonzlez de San Romn, Beatriz Abad, Ivn Manuel, and Rafael Rodrguez-Puertas Part V Protein Analyses of Human Brain 14. The Use of Affinity Purification-Mass Spectrometry to identify Phosphorylates Tau Interactors in Alzheimer’s Disease Geoffrey Pires, Beatrix Ueberheide, Thomas Wisniewski, and Eleanor Drummond 15. In Vitro Amplification of Pathogenic Tau Seeds from Neurodegenerative Disease Patient Brains Hong Xu and Virginia M.-Y. Lee 16. A and Tau Prions Causing Alzheimer’s Disease Carlo Condello, Gregory E. Merz, Atsushi Aoyagi, William F. DeGrado, and Stanley B. Prusiner Subject Index List…
