Description
This volume provides state-of-the-art and novel methods on antibiotic isolation and purification, identification of antimicrobial killing mechanisms, and methods for the analysis and detection of microbial adaptation strategies. Antibiotics: Methods and Protocols guides readers through chapters on production and design, mode of action, and response and susceptibility. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Antibiotics: Methods and Protocols aims to inspire scientific work in the exciting field of antibiotic research. Part I: Production and Design 1. Antibiotics – Precious Goods in Changing Times Peter Sass 2. Mining Bacterial Genomes for Secondary Metabolite Gene Clusters Martina Adamek, Marius Spohn, Evi Stegmann, and Nadine Ziemert 3. Production of Antimicrobial Compounds by Fermentation Henrik Harms, Gabriele M. Knig and Till F. Schberle 4. Structure Elucidation of Antibiotics by NMR Spectroscopy Georgios Daletos, Elena Ancheeva, Raha Orfali, Victor Wray, and Peter Proksch 5. Computer-Aided Drug Design Methods Wenbo Yu and Alexander D. MacKerell, Jr. 6. Cytotoxicity Assays as Predictors of the Safety and Efficacy of Antimicrobial Agents Alexander Zipperer and Dorothee Kretschmer Part II: Mode of Action 7. Application of a Bacillus subtilis Whole-cell Biosensor (PliaI-lux) for the Identification of Cell Wall Active Antibacterial Compounds Carolin Martina Kobras, Thorsten Mascher, and Susanne Gebhard 8. Determination of Bacterial Membrane Impairment by Antimicrobial Agents Miriam Wilmes and Hans-Georg Sahl 9. Mass-sensitive Biosensor Systems to Determine the Membrane Interaction of Analytes Sebastian G. Ho and Gerd Bendas 10. Measurement of Cell Membrane Fluidity by Laurdan GP:Fluorescence Spectroscopy and Microscopy Kathi Scheinpflug, Oxana Krylova and Henrik Strahl 11. In vitro Assays to Identify Antibiotics Targeting DNA Metabolism12. Fluorescence-based real-time Activity Assays to Identify RNase P inhibitors Yu Chen, Xin Liu, Nancy Wu, and Carol A. Fierke 13. Reporter Gene-based Screening for TPP Riboswitch Activators Christina E. Lnse and Gnter Mayer 14. Cell-based Fluorescent Screen to Identify Inhibitors of Bacterial Translation Initiation Federica Briani 15. Bacterial Histidine Kinases: Overexpression, Purification, and Inhibitor Screen Mike Gajdiss, Michael Trck, and Gabriele Bierbaum Part III: Response and Susceptibility 16. Expression Profiling of Antibiotic Resistant Bacteria Obtained by Laboratory Evolution Shingo Suzuki, Takaaki Horinouchi, and Chikara Furusawa 17. Sample Preparation for Mass-Spectrometry Based Absolute Protein Quantification in Antibiotic Stress Research Florian Bonn, Sandra Maass, and Drte Becher 18. Label-Free Quantitation of Ribosomal Proteins from Bacillus Subtillis for Antibiotic Research Sina Schkermann, Pascal Prochnow, and Julia E. Bandow 19. Functional Metagenomics to Study Antibiotic Resistance Manish Boolchandani, Sanket Patel, and Gautam Dantas 20. Epidemiological Surveillance and Typing Methods to Track Antibiotic Resistant Strains using High Throughput Sequencing Miguel Paulo Machado, Bruno Ribeiro-Gonalves, Mickael Silva, Mrio Ramirez, Joo Andr Carrio
