Description
This volume covers a wide range of model systems from invertebrate to humans and in vitro and in vivo. Chapters detail methods on squid, aplysia, xenopus, mouse in vivo, ex vivo, rodent primary neurons, human-derived neurons, zebrafish, drosophila in vivo, primary neurons, c. elegans, and in vitro reconstitution assays.Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Axonal Transport: Methods and Protocols aims to further our understanding of the trafficking of cellular components in the nervous system leading to the advancement of basic knowledge that underlines the cell biology of the neuron. The chapters ” Live imaging of RNA Transport and Translation in Xenopus Retinal Axons, Retrograde Axonal Transport of Neurotrophins in Basal Forebrain Cholinergic Neurons, Assessment of Mitochondrial Trafficking as A Surrogate of Fast Axonal Transport in Human Induced Pluripotent Stem Cell Derived Spinal Motor Neurons, Drosophila Primary Neuronal Cultures as a Useful Cellular Model to Study and Image Axonal Transport, and In vitro Reconstitution of Kinesin-Based, Axonal mRNA Transport” are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com Part I: Squid, Aplysia, and Xenopus 1. The Giant Axon of the Squid: A Simple System for Axonal Transport Studies Joseph DeGiorgis, Marcus Jang, and Elaine L Bearer 2. Live Imaging and Quantitative Analysis of Organelle Transport In Sensory Neurons of Aplysia Californica Kerriann Badal, Yibo Zhao, Kyle Miller, and Sathyanarayanan Puthanveettil 3. Live imaging of RNA Transport and Translation in Xenopus Retinal Axons Julie Qiaojin Lin and Jean-Michel Cioni Part II: Mouse in vivo and ex vivo 4. Imaging Axonal Transport in Ex Vivo Central and Peripheral Nerves Stacey Anne Gould, Robert Adalbert, Stefan Milde, and Michael Coleman 5. In vivo Imaging of Axonal Organelle Transport in the Mouse Brain Johannes Knabbe, Jil Protzmann, and Thomas Kuner 6. Studying Axonal Transport in the Brain by Manganese-Enhanced Magnetic Resonance Imaging (MEMRI) Elaine L. Bearer, Xiaowei Zhang,and Russell E. Jacobs Part III: Rodent Primary Neurons 7. Microfluidic Neuromuscular Co-Culture System for Tracking Cell-To-Cell Transfer and Axonal Transport of Labeled Proteins Ariel Ionescu and Eran Perlson 8. Imaging Diversity in Slow Axonal Transport Archan Ganguly and Subhojit Roy 9. Methods and Applications of Campenot Trichamber Neuronal Cultures for the Study of Neuroinvasive Viruses Wesley M. Tierney, Ian A. Vicino, Stella Y. Sun, Wah Chiu, Esteban A. Engel, Matthew P. Taylor, and Ian B. Hogue 10. Molecular Analysis of Axonal Transport Dynamics Upon Modulation of Microtubule Acetylation Silvia Turchetto, Romain Le Bail, Loic Broix, and Laurent Nguyen 11. Live-cell Imaging of RNA Transport In Axons Of Cultured Primary Neurons Tabitha J. Hees and Angelika B. Harbauer 12. Visualizing Vesicle-Bound Kinesins in Cultured Hippocampal Neurons Andrew Montgomery, Alex Garbouchian, and Marvin Bentley 13. Retrograde Axonal Transport of Neurotrophins in Basal Forebrain Cholinergic Neurons. Arman Shekari and Margaret Fahnestock 14. Use of Microfluidics Chambers to Image Axonal Transport In Adult Sensory Neurons Maria Fransiska Emily, Lokesh Agrawal, Paolo Barzaghi, Miki Otsuki, and Marco Terenzio Part IV: Human-derived Neurons 15. High-resolution Imaging of Mitochondria and Mitochondrial Nucleoids in Differentiated SH-SY5Y Cells Emily Annuario, Kristal Y-W Ng, and Alessio Vagnoni 16. Assessment of Mitochondrial Trafficking as A Surrogate of Fast Axonal Transport in Human Induced Pluripotent Stem Cell Derived Spinal Motor Neurons Arpan R. Mehta, Siddharthan Chandran, and Bhuvaneish T. Selvaraj Part V: Zebrafish 17. In vivo Live Imaging of Axonal Transport In Developing Zebrafish Axons Melody Atkins, Jamil Hazan, and Coralie Fassier 18. Visualizing the Intracellular Trafficking in Zebrafish Mauthner Cells Rongchen Huang, Yang Xu, Min Chen, Leiqing Yang, Xinliang Wang, Yueru Shen, Yubin Huang, Bing Hu Part VI: Drosophila In Vivo, Ex Vivo, and Primary Neurons 19. Dissection and Direct Imaging of Axonal Transport in Drosophila Segmental Nerves William M. Saxton, Angeline Lim, and Inna Djagaeva 20. Detailed Imaging of Mitochondrial Transport and Precise Manipulation of Mitochondrial Function with Genetically-Encoded Photosensitizers in Adult Drosophila Neurons Francesca Mattedi, George Chennell, and Alessio Vagnoni 21. Mitochondrial DNA Transport in Drosophila neurons Joseph M. Bateman 22. Live imaging of Axonal Transport in the Adult Drosophila Central Nervous System Wayne Robinson and Tanja A Godenschwege 23. Drosophila Primary Neuronal Cultures as a Useful Cellular Model to Study and Image Axonal Transport Andr Voelzmann and Natalia Snchez-Soriano 24. High-resolution Live-Imaging of Axonal RNP granules in Drosophila Pupal Brain Explants Caroline Medioni, Jeshlee Vijayakumar, Anne Ephrussi, and Florence Besse Part VII: C. elegans 25. Analyzing the Impact of Gene Mutations on Axonal Transport in Caenorhabditis elegans Yuzu Anazawa and Shinsuke Niwa 26. Methods to Quantify and Relate Axonal Transport Defects to Changes In C. Elegans Behavior Syed Nooruzuha Barmaver, Muniesh Muthaiyan Shanmugam, and Oliver Ingvar Wagner 27. Imaging Intracellular Trafficking in Neurons of C. elegans. Sravanthi S P Nadiminti and Sandhya P Koushika Part VIII: In vitro Reconstitution Assays 28. In vitro Reconstitution of Molecular Motor-Driven Mitochondrial Transport Verena Henrichs, Jakub Rohlena, Marcus Braun, and Zdenek Lansky 29. In vitro Reconstitution of Kinesin-Based, Axonal mRNA Transport Julia Grawenhoff, Sebastian Baumann, and Sebastian P. Maurer
