Description
The second edition of Bone Marrow and Stem Cell Transplantation expands upon the previous edition with current, detailed methods on HLA, minor-HLA and Killer Immunoglobulin Like Receptor typing. With new chapters on immunophenotyping and functional characterization of stem cells are included. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Bone Marrow and Stem Cell Transplantation, Second Edition serves as a guide in the application of molecular methods for routine or investigational purposes. 1. Reporter Gene Technologies for Imaging Cell Fates in Hematopoiesis Sophie Kusy and Christopher H. Contag 2. Flow Cytometry for Hematopoietic Cells Daniela S. Krause, Michelle E. DeLelys, Frederic I. Preffer 3. Isolation and Characterization of Mesenchymal Stem Cells Sedat Odabas, A. Eser Elcin, and Y. Murat Elcin 4. Methods for Functional Analysis of Stem Cells Aurore Saudemont, Michelle Escobedo-Cousin and J Alejandro Madrigal 5. An Overview of HLA Typing for Heamatopoietic Stem Cell Transplantation Katy Latham, Ann-Margaret Little, J Alejandro Madrigal 6. HLA Typing with Sequence-Specific Oligonucleotide Primed PCR (PCR-SSO) and Use of the Luminex(TM) Technology Klara Dalva and Meral Beksac 7. Sequence Based Typing of HLA: An Improved Group Specific Full Length Gene Sequencing Approach Christina E.M. Voorter, Fausto Palusci and Marcel G.J. Tilanus 8. Molecular Typing Methods for Minor Histocompatibility Antigens Eric Spierings 9. Natural Killer Cells and Killer-cell Immunoglobulin-like Receptor Polymorphisms: Their Role in Hematopoietic Stem Cell Transplantation Jennifer Schellekens, Katia Gagne, and Steven GE Marsh 10. Methods of Detection of Immune Reconstitution and T Regulatory Cells by Flow Cytometry Richard Charles Duggleby and J Alejandro Madrigal 11. Molecular Methods Used for Detection of Minimal Residual Disease Following Hematopoietic Stem Cell Transplantation in Myeloid Disorders Ahmet H. Elmaagacli 12. Molecular Methods for Detection of Minimal Residual Disease Following Transplantation in Lymphoid and Plasma Cell Disorders Paolo Corradini and Cristiana Carniti 13. Molecular Methods for Detection of Invasive Fungal Infections and Mycobacteria and Their Clinical Significance in Hematopoietic Stem Cell Transplantation Pinar Yurdakul and Sule Colakoglu 14. Post-transplant Monitoring of Chimerism by Lineage-specific Analysis Sandra Preuner and Thomas Lion 15. Urinary Proteomics Employing Capillary Electrophoresis Coupled to Mass Spectrometry in The Monitoring Of Patients After Stem Cell Transplantation Eva M. Weissinger, William Mullen, and Amaya Albalat
