Description
This volume discusses the latest advancements and technologies used in cancer drug resistance research. Cancer Drug Resistance: Overviews and Methods contains chapters that cover topics such as: studying the mechanics of resistance to DNA damaging therapeutic drugs; studies to delineate the role of efflux transporters; expression of drug transporters; resistance to targeted therapies in breast cancer; the role of microRNAs in current pancreatic cancer treatment; and cancer exosomes as mediators of drug resistance or clinical and molecular methods in drug development and the use of bioinformatics in the management of cancer drug resistance data. Written in the highly successful Methods in Molecular Biology series format, chapters include overviews of the main issues in cancer drug resistance and the respective mechanisms, as well as introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cancer Drug Resistance: Overviews and Methods, is a valuable resource to researchers, oncobiologists and clinical oncologists or anyone else who is interested in the study of cancer and its drug resistances. 1. Cancer Drug Resistance: A Brief Overview from a Genetic Viewpoint Jos Rueff and Antnio S. Rodrigues 2. Classical and Targeted Anti-Cancer Drugs: An Appraisal of Mechanisms of Multidrug Resistance Bruce C. Baguley 3. In Vitro Methods for Studying the Mechanisms of Resistance to DNA Damaging Therapeutic Drugs Pasarat Khongkow, Anna K. Middleton, Jocelyn P.-M. Wong, Navrohit K. Kandola, Mesayamas Kongsema, Gabriela Nestal de Moraes, Ana R. Gomes, and Eric W.-F. Lam 4. In Vitro Approaches to Study Regulation of Hepatic Cytochrome P450 (CYP) 3A Expression by Paclitaxel and Rifampicin Romi Ghose, Pankajini Mallick, Guncha Taneja, Chun Chu, and Bhagavatula Moorthy 5. Uptake and Permeability Studies to Delineate the Role of Efflux Transporters Ramya Krishna Vadlapatla, Dhananjay Pal, and Ashim K. Mitra 6. Dynamics of Expression of Drug Transporters: Methods for Appraisal Marta Gromicho, Jos Rueff, and Antnio S. Rodrigues 7. Fluorimetric Methods for Analysis of Permeability, Drug Transport Kinetics, and Inhibition of the ABCB1 Membrane Transporter Ana Armada, Clia Martins, Gabriella Spengler, Joseph Molnar, Leonard Amaral, Antnio S. Rodrigues, and Miguel Viveiros 8. Resistance to Targeted Therapies in Breast Cancer Sofia Braga 9. MicroRNAs and Cancer Drug Resistance Bruno Costa Gomes, Jos Rueff, and Antnio S. Rodrigues 10. The Role of MicroRNAs in Resistance to Current Pancreatic Cancer Treatment: Translational Studies and Basic Protocols for Extraction and PCR Analysis Ingrid Garajov, Tessa Y.S. Le Large, Elisa Giovannetti, Geert Kazemier, Guido Biasco, and Godefridus J. Peters 11. Methods for Studying MicroRNAs Expression and Their Targets in Formalin-Fixed, Paraffin-Embedded (FFPE) Breast Cancer Tissues Bruno Costa Gomes, Bruno Santos, Jos Rueff, and Antnio S. Rodrigues 12. The Regulatory Role of Long Non-Coding RNAs in Cancer Drug Resistance Marjan E. Askarian-Amiri, Euphemia Leung, Graeme Finlay, and Bruce C. Baguley 13. Cancer Exosomes as Mediators of Drug Resistance Maria do Rosrio Andr, Ana Pedro, and David Lyden 14. Isolation and Characterization of Cancer Stem Cells from Primary Head and Neck Squamous Cell Carcinoma Tumors Hong S. Kim, Alexander T. Pearson, and Jacques E. Nr 15. Clinical and Molecular Methods in Drug Development: Neoadjuvant Systemic Therapy in Breast Cancer as a Model Sofia Braga 16. Proteomics in the Assessment of the Therapeutic Response of Anti-Neoplastic Drugs: Strategies and Practical Applications Vukosava Milic Torres, Lazar Popovic, Ftima Vaz, and Deborah Penque 17. Managing Drug Resistance in Cancer: Role of Cancer Informatics Ankur Gautam, Kumardeep Chaudhary, Rahul Kumar, Sudheer Gupta, Harinder Singh, and Gajendra P.S. Raghava
