Description
Part I Culturing and morphology 1. Rat pheochromocytoma PC12 cells in culture Chin-Tien Wang 2. Adrenal grafts in the central nervous system: Chromaffin and chromaffin progenitor cell transplantation Alejandra Boronat-Garcia, Marcela Palomero-Rivero, Magdalena Guerra-Crespo 3. Immunocytochemistry of acutely isolated adrenal medullary chromaffin cells Keita Harada, Hidetada Matsuoka, Masumi Inoue 4. Transmission electron microscopy: a method for studying the adrenochromaffin cells Anna Capaldo, Maria De Falco, Luigi Rosati and Vincenza Laforgia 5. Immunogold for protein location in chromaffin cells Rafael Lujn, Rocio Alfaro-Ruiz and Carolina Aguado 6. Visualization of exo- and endocytosis membrane dynamics with super-resolution STED microscopy Chung Yu Chan, Sue Han, Xin Wang, Xiaoli Guo and Ling-Gang Wu Part II Stimulus-secretion coupling 7. Online detection of catecholamine release from the perfused rat adrenal gland Ricardo Borges, Ana de la Iglesia and Antonio G. Garca 8. On-line recording of perifused chromaffin cells Ricardo De Pascual and Luis Ganda 9. Recording of chromaffin cell electrical activity in situ in acute adrenal slices Nathali Guerineau 10. Calcium imaging and amperometric recording in cultured chromaffin cells and adrenal slices from normotensive, Wistar Kyoto rats, and Spontaneously Hypertensive Rats Tzitzitlini Alejandre-Garca, Pedro Segura-Chama, Oscar Javier Parada-Parra, Diana Milln-Aldaco and Arturo Hernndez-Cruz. 11. Measurements of calcium in chromaffin cell organelles using targeted aequorins. Jaime Santo-Domingo, Pilar lvarez-Illera, Pablo Montenegro, Rosalba I Fonteriz, Mayte Montero and Javier lvarez 12. Quantification of secretory granule exocytosis by TIRF imaging and capacitance measurements Muhmmad Omar-Hmeadi, Liangwen Liu, Santiago Echeverry, and Sebastian Barg 13. Membrane capacitance measurements of stimulus-evoked exocytosis in adrenal chromaffin cell Elizabeth P. Seward and Robert C. Wykes 14. Quantal release analysis of electrochemically active molecules using single-cell amperometry Natalia Domnguez, Pablo Montenegro and J. David Machado 15. Methodologies for detecting quantal exocytosis in adrenal chromaffin cells through diamond-based MEAs Giulia Tomagra, Claudio Franchino, Emilio Carbone, Andrea Marcantoni, Alberto Pasquarelli, Federico Picollo, Valentina Carabelli 16. Vesicle collision protocols for the study of quantum size and exocytotic fraction released Soodabeh Majdi, Alex S. Lima and Andrew Ewing 17. Patch Amperometry and Intracellular Patch Electrochemistry Eugene V. Mosharov and Manfred Lindau 18. Artificial cells for dissecting exocytosis Yuanmo Wang and Ann-Sofie Cans Part III Chromaffin granules and membrane traffic 19. Isolation and purification of chromaffin granules from adrenal glands and cultured neuroendocrine cells Arlek Gonzalez-Jamett, Mara Constanza Maldifassi, Ana Mara Crdenas 20. Confocal microscopy studies of F-actin cytoskeleton distribution and dynamics using fluorescent LifeAct constructs in bovine adrenal chromaffin cells Jos Villanueva, Yolanda Gimenez-Molina and Luis M. Gutirrez 21. Unveiling the nanoscale dynamics of the exocytic machinery in chromaffin cells with single-molecule imaging Adekunle T. Bademosi and Frdric A. Meunier Part IV Miscellanea 22. Determination of catecholamines in a small volume (25l) of plasma from conscious mouse tail vein Kechun Tang and Sushil K. Mahata 23. Quantification of chromogranin A and its fragments in biological fluids Flavio Curnis, Barbara Colombo and Angelo Corti 24. Cytotoxicity models in chromaffin cells to evaluate neuroprotective compounds Mara F. Cano Abad and Manuela G. Lpez
