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Series Preface… Preface… Table of Contents… Contributing Authors… Part I Experimental Manipulation of the Inner Ear 1. Pou4f3DTR Mice Enable Selective and Timed Ablation of Hair Cells in Post-Natal Mice Jennifer S. Stone, Edwin W. Rubel, and Mark E. Warchol 2. Cochlear Explant Cultures: Creation and Application Elizabeth Carroll Driver and Matthew W. Kelley 3. Immunohistochemistry and In Situ mRNA Detection using Inner Ear Vibratome Sections Mirko Scheibinger, Amanda Janesick, Giovanni H. Diaz, and Stefan Heller 4. Genetic Manipulation of the Embryonic Chicken Inner Ear Nicolas Daudet, Magdalena Zak, Thea Stole, and Stephen Terry 5. Molecular Tools to Study Regeneration of the Avian Cochlea and Utricle Amanda Janesick, Mirko Scheibinger, and Stefan Heller Part II Molecular Analysis of the Inner Ear 6. An Efficient Method to Detect Messenger RNA (mRNA) in the Inner Ear by RNAscope In Situ Hybridization Sumana Ghosh, Graham Casey, Kendra L. Stansak, Punam Thapa, and Bradley J. Walters 7. A Manual Technique for Isolation and Single-Cell RNA Sequencing Analysis of Cochlear Hair Cells and Supporting Cells Cody West, Paul T. Ranum, Ryotaro Omichi, Yoichiro Iwasa, Daniel Walls, Jin-Young Koh, and Richard J.H. Smith 8. Ribosomal Pulldown Assays and Their Use to Analyze Gene Expression in Multiple Inner Ear Cell Types Maggie S. Matern, Beatrice Milon, Ran Elkon, and Ronna Hertzano Part III Hair Cell Function and Physiology 9. In Vivo Analysis of Hair Cell Sensory Organs in Zebrafish: From Morphology to Function Saman Hussain, Roberto Aponte-Rivera, Rana M. Barghout, Josef G. Trapani, and Katie Kindt 10. Electrophysiological Recordings of Voltage Dependent and Mechanosensitive Currents in Sensory Hair Cell of the Auditory and Vestibular Organs of the Mouse Artur A. Indzhykulian, Stuart L. Johnson, and Gwenalle S.G. Gloc 11. Biophysical Recording from Adult Hair Cells Antonio Miguel Garcia de Diego and Jonathan F. Ashmore Part IV The Central Auditory Pathway 12. Endocochlear Potential Measures, Local Drug Application, and Perilymph Sampling in the Mouse Inner Ear Kevin K. Ohlemiller, Jared J. Hartsock, and Alec N. Salt 13. In Vivo Whole-Cell Recording in the Gerbil Cochlear Nucleus Hsin-Wei Lu and Philip Joris 14. Measurement of Human Cochlear and Auditory Nerve Potentials Eric Verschooten and Philip X. Joris 15. Strategies for Identification of Medial Olivocochlear Neurons for Patch-Clamp Studies of Synaptic Function using Electrical Stimulation and Optogenetics Kirupa Suthakar, Lester Torres Cadenas, and Catherine Weisz 16. Auditory Brainstem Response (ABR) Measurements in Small Mammals Ye-Hyun Kim, Katrina M. Schrode, and Amanda M. Lauer 17. Behavioral Models Loudness, Hyperacusis, and Sound Avoidance Richard Salvi, Connor Mauche, Hannah Thorner, Guang-Di Chen, and Senthilvelan Manohar Subject Index List…
