Description
The discipline of developmental toxicology is an integration of concepts, models, and methodologies based heavily on the superimposition of toxicology principles upon the science of developmental biology. The science of developmental toxicology also borrows from other research areas that are concerned with regulation of cell growth, migration, differentiation and cell death, as such are central to the study of stem cells, cancer, and chronic diseases. In Developmental Toxicology: Methods and Protocols expert researchers in the field detail many of the methods which are now commonly used to study developmental toxicology highlighting the evolution of methods from classical teratology approaches to the dynamic, state-of-the-art molecular methods, systems biology, and next generation models and procedures. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Developmental Toxicology: Methods and Protocols is a valuable resource to those planning experiments to investigate consequences of environmental, nutritional, or chemical effects caused during development. 1. Volume Overview – Introduction Craig Harris 2. In Vivo Models of Developmental Toxicology Jason M. Hansen 3. Caenorhabditis Elegans as a Model in Developmental Toxicology Windy A. Boyd, Marjolein V. Smith, and Jonathan H. Freedman 4. Zebrafish Embryo Developmental Toxicology Assay Julieta M. Panzica-Kelly, Cindy X. Zhang, Karen Augustine-Rauch 5. Gene Knockdown by Morpholino-modified Oligonucleotides in the Zebrafish (Danio rerio) Model: Applications for Developmental Toxicology Alicia R. Timme-Laragy, Sibel I. Karchner, Mark E. Hahn 6. An Amphibian Model for Studies of Developmental Reproductive Toxicity Cecilia Berg 7. Avian Models in Teratology and Developmental Toxicology Susan M. Smith, George R. Flentke, Ana Garic 8. Overview of In Vitro Models in Developmental Toxicology Craig Harris 9. Primary Cell and Micromass Culture in Assessing Developmental Toxicity M Pratten, Bhavesh K. Ahir, H. Smith-Hurst, S Memon, P Mutch & P.Cumberland 10. Embryonic Stem Cell Test (EST): Stem cell Use in Predicting Developmental Cardiotoxicity and Osteotoxicity Batrice Kuske, Polina Y. Pulyanina, Nicole I. zur Nieden 11. Mouse Embryonic Stem Cell Adherent Cell Differentiation and Cytotoxicity (ACDC) Assay Marianne Barrier, Kelly Chandler, Susan Jeffay, Maria Hoopes, Tom Knudsen, Sid Hunter 12. The Murine Limb Bud in Culture as an In Vitro Teratogenicity Test System France-Hlne Paradis, Chunwei Huang and Barbara F. Hales 13. Rodent Whole Embryo Culture Craig Harris 14. Rabbit Whole Embryo Culture Valeris A. Marshall and Edward W. Carney 15. Assessment of Xenobiotic Biotransformation Including Reactive Oxygen Species Generation in the Embryo using Benzene as an Example Helen J. Renaud, Allison Rutter, and Louise M. Winn 16. Methodological Approaches to Cytochrome P450 Profiling in Embryos Jared V. Goldstone and John J. Stegeman 17. Analysis of Nrf2-Mediated Transcriptional Induction of Antioxidant Response in Early Embryos Shao-yu Chen 18. Regulation and Control of AP-1 Binding Activity in Embryotoxicity Terence R.S. OzolinS 19. Thioredoxin Redox Status Assessment During Embryonic Development: The Redox Western Jason M. Hansen 20. Methods for the Determination of Plasma or Tissue Glutathione Levels Trent E. Tipple and Lynette K. Rogers 21. Oxidative Stress, Thiols, and Redox Profiles Craig Harris and Jason M. Hansen 22. A Review of Toxicogenomic Approaches in Developmental Toxicology Joshua F. Robinson, Jeroen L.A. Pennings, Aldert H. Piersma 23. Epigenetic Approaches and Methods in Developmental Toxicology: Role of Inhibition in Teratogenic Events Elena Menegola, Graziella Cappelletti and Francesca Di Renzo 24. DNA Methylation Screening and Analysis Karilyn E. Sant, Muna S. Nahar, and Dana C. Dolinoy 25. Assessment of Histiotrophic Nutrition using Fluorescent Probes Jeffrey Ambroso and Craig Harris 26. Diabetic Embryopathy Ulf J. Eriksson and Parri Wentzel 27. Gene Expression Analysis in Developing Embryos: In situ Hybridization Siew-Ging Gong 28. Assessment of Gross Fetal Malformations: The Modernized Wilson Technique and Skeletal Staining BRobert E. Seegmiller, Nathan Cook, Korance Goodwin, and Timothy Leishman
