Description
To foster a better understanding of dopamine receptor functionality, this detailed volume creates an interface between updated classical methods and new emerging technologies heretofore not available to new or seasoned researchers. Divided in five sections dedicated to experimental approaches investigating different facets of dopaminergic signal transduction, Dopamine Receptor Technologies covers epigenetic and post-transcriptional analysis, computational and biochemical techniques, visualization and imaging methods, molecular and cell biological tools, as well as behavioral assessment. The book, as a part of the popular Neuromethods series, provides insightful step-by-step protocols and methodological reviews that readers will find useful. Practical and versatile, Dopamine Receptor Technologies seeks to aid researchers in developing new pharmacological tools to improve our knowledge of in vivo roles played by each receptor subtype and the synthesis of prospective lead compounds for drug discovery. Part I: Genetic, Epigenetic, and Post-Transcriptional Analysis of Dopamine Receptors 1. Genetic and Epigenetic Methods for Analysis of the Dopamine D2 Receptor Gene Laura M. Fiori, Mireille Daigle, and Paul R. Albert 2. Characterization of D1 Dopamine Receptor Post-Transcriptional Regulation Eldo V. Kuzhikandathil Part II: Computational and Biochemical Methods in the Investigation of Dopamine Receptor Structure, Binding, and Post-Translational Regulation 3. Computational Approaches in the Structure-Function Studies of Dopamine Receptors Mayako Michino and Lei Shi 4. Cell-Free Protein Synthesis and Purification of the Dopamine D2 Receptor Dipannita Basu, Ritesh Daya, Christal D.R. Sookram, and Ram K. Mishra 5. Wnt Ligand Binding to and Regulation of Dopamine D2 Receptors Sehyoun Yoon, Mi-Hyun Choi, and Ja-Hyun Baik 6. Regulation of Pre- and Postsynaptic Protein Phosphorylation by Dopamine D2 Receptors Alessandra Bonito-Oliva, Giada Spigolon, and Gilberto Fisone 7. Study of Dopamine D1 Receptor Regulation by G Protein-Coupled Receptor Kinases Using Whole-Cell Phosphorylation and Cross-Linking Methods Keyvan Sedaghat, Boyang Zhang, Xiaodi Yang, Caroline Lefebvre, and Mario Tiberi 8. Ubiquitination of Dopamine Receptor Studied by Sequential Double Immunoprecipitation Kamila Skieterska, Pieter Rondou, and Kathleen Van Craenenbroeck Part III: Visualization and Imaging of Dopamine Receptors and Ligands 9. Dopamine Receptors in the Subthalamic Nucleus: Identification and Localization of D5 Receptors Lionel Froux, Diana Suarez-Boomgaard, Jerome Baufreton, Alicia Rivera, Maurice Garret, and Anne Taupignon 10. MALDI Mass Spectrometry Imaging of Dopamine and PET D1 and D2 Receptor Ligands in Rodent Brain Tissues Richard J.A. Goodwin, Mohammadreza Shariatgorji, and Per E. Andren 11. Positron Emission Tomography Imaging of Dopaminergic Receptors in Rats Boguslaw Szczupak and Abraham Martn Part IV: Molecular and Cell Biological Approaches in the Study of Dopamine Receptor Function 12. Transactivation of Receptor Tyrosine Kinases by Dopamine Receptors Jeff S. Kruk, Azita Kouchmeshky, Nicholas Grimberg, Marina Rezkella, and Michael A. Beazely 13. Dopamine Receptors in Human Embryonic Stem Cell Differentiation Glenn S. Belinsky, Mandakini B. Singh, Katerina D. Oikonomou, Michele L. McGovern, and Srdjan D. Antic 14. Calcium and PLC Signaling Through Dopamine Receptors Lani S. Chun, R. Benjamin Free, and David R. Sibley 15. Intracellular Trafficking Assays for Dopamine D2-Like Receptors Chengchun Min, Mei Zheng, and Kyeong-Man Kim 16. Study of Crosstalk Between Dopamine Receptors and Ion Channels Ping Su, Albert H.C. Wong, and Fang Liu Part V: Behavioral Analysis of Dopamine Function 17. Study of Dopamine Receptor and Dopamine Transporter Networks in Mice Victor Gorgievski, Eleni T. Tzavara, and Bruno Giros 18. Optogenetic Regulation of Dopamine Receptor-Expressing Neurons T. Chase Francis and Mary Kay Lobo 19. Characterization of D3 Dopamine Receptor Agonist-Dependent Tolerance Property Samantha R. Cote and Eldo V. Kuzhikandathil 20. Dopamine D1 and D2 Receptors in Chronic Mild Stress: Analysis of Dynamic Receptor Changes in an Animal Model of Depression Using In Situ Hybridization and Autoradiography Dariusz Zurawek, Agata Faron-Grecka, Maciej Kusmider, Joanna Solich, Magdalena Kolasa, Kinga Szafran, Katarzyna Kmiotek, Piotr Gruca, Mariusz Papp, and Marta Dziedzicka-Wasylewska
