Description
A compendium of proven experimental approaches and strategies for studying the bioactivation, detoxification, tissue distribution, and elimination of xenobiotics in the metabolism and/or transport of various chemicals. The authors address several of the major drug metabolizing systems, including the cytochrome P450 family, flavin-containing monooxygenases, glutathione, S-transferase, glucuronidation, N-acetylation, and sulfotransferases. Additional chapters present novel approaches to the study of: signaling pathways in the regulation of drug metabolism enzymes, how the modulation of thiols and other low molecular-weight cofactors can alter drug metabolism, and how modulation of drug metabolism pathways can influence antiviral therapy. Renal Cytochrome P450s and Flavin-Containing Monooxygenases: Potential Roles in Metabolism and Toxicity of 1,3-Butadiene, Trichloroethylene, and Tetrachloroethylene Adnan A. Elfarra Experimental Approaches for the Study of Cytochrome P450 Gene Regulation Hollie I. Swanson, Susan D. Kraner, Soma S. Ray, Martin Hoagland, Earl D. Thompson, Xinyu Zheng, and Yanan Tian Insulin and Growth Factor Signaling: Effects on Drug-Metabolizing Enzymes Sang K. Kim, Kimberley J. Woodcroft, and Raymond F. Novak Catalytic Function and Expression of Glutathione Transferase Zeta Philip G. Board, M. W. Anders, and Anneke C. Blackburn Glucuronidation of Fatty Acids and Prostaglandins by Human UDP-Glucuronosyltransferases Anna Radominska-Pandya, Joanna M. Little, and Arthur Bull Transcriptional Regulation of UDP- Glucuronosyltransferases Anna Radominska-Pandya, Peter I. Mackenzie, and Wen Xie Phenotypic and Genotypic Characterization of N-Acetylation Craig K. Svensson and David W. Hein Methods and Approaches to Study Metabolism and Toxicity of Acetaminophen Sam A. Bruschi Modulation of Drug Metabolism and Antiviral Therapies Bernhard H. Lauterburg Modulation of Thiols and Other Low-Molecular-Weight Cofactors: Effects on Drug Metabolism and Disease Susceptibility Charles V. Smith Multidrug Resistance Proteins and Hepatic Transport of Endo- and Xenobiotics Phillip M. Gerk and Mary Vore Structural Determinants of Folate and Antifolate Membrane Transport by the Reduced Folate Carrier Wei Cao and Larry H. Matherly Glutathione Transport in the Kidneys: Experimental Models, Mechanisms, and Methods Lawrence H. Lash Human Cytosolic Sulfotransferases: Properties, Physiological Functions, and Toxicology Charles N. Falany Index
