Description
1. Need for Methods to Investigate Endocannabinoid Signaling Mauro Maccarrone 2. Extraction and Simultaneous Quantification of Endocannabinoids and Endocannabinoid-like Lipids in Biological Tissues Claudia Schwitter, Beat Lutz, and Laura Bindila 3. Measuring the Content of Endocannabinoid-like Compounds in Biological Fluids: A Critical Overview of Sample Preparation Methodologies Heather B Bradshaw and Clare T Johnson 4. LC-MS/MS Analysis of AEA and 2-AG Natalia Battista, Federico Fanti, and Manuel Sergi 5. Analysis of Omega-3 Fatty Acid Derived N-Acylethanolamines in Biological Matrices Renger F. Witkamp, Ian de Bus, Bauke Albada, and Michiel G.J. Balvers 6. Endocannabinoid-Binding Receptors as Drug TargetsMara Gmez-Caas, Carmen Rodrguez-Cueto, Valentina Satta, Ins Hernndez-Fisac, Elisa Navarro, and Javier Fernndez-Ruiz 7. Assay of CB1 Receptor Binding Valeria Gasperi, Isabella Savini and Maria Valeria Catani 8. The Displacement Binding Assay Using Human Cannabinoid CB2 Receptor-Transfected Cells Maria Grazia Cascio, Pietro Marini and Roger G. Pertwee 9. Fluorescence-based Assay for TRPV1 Channels Aniello Schiano Moriello, Luciano De Petrocellis, Rosa Maria Vitale 10. DNA-Protein-Interaction (DPI)-ELISA Assay for PPAR- Receptor Binding Maria Valeria Catani, Valentina Tullio, Mauro Maccarrone, and Valeria Gasperi 11. Scintillation Proximity Assay (SPA)-Based Radioligand Binding for PPARa, PPAR and PPARd Receptors Uwe Grether, Jrg Benz, Thomas Hartung, and Doris Roth 12. A Homogeneous Time-Resolved Fluorescence Resonance Energy Transfer (TR-FRET) Cofactor Recruitment Assay for PPARa and PPAR Doris Roth, Jrg Benz, Uwe Grether, and Michel Dietz 13. The Cyclic AMP Assay Using Human Cannabinoid CB2 Receptor-Transfected Cells Pietro Marini, Maria Grazia Cascio, and Roger G. Pertwee 14. Assay of GTPS Binding in Autoradiography Marina Gabaglio, Pamela Prini, Erica Zamberletti, Tiziana Rubino, and Daniela Parolaro 15. Cellular Assay to Study -Arrestin Recruitment by the Cannabinoid Receptors 1 and 2 Jara Bouma, Marjolein Soethoudt, Noortje van Gils1, Lizi Xia1, Mario van der Stelt2, 3 and Laura H. Heitman 16. Endocannabinoid Metabolism and Transport as Drug Targets Francesca Ciaramellano, Federico Fanti, Lucia Scipioni, Mauro Maccarrone, and Sergio Oddi 17. Assay of NAT Activity Toru Uyama and Natsuo Ueda 18. Radiometric Assay of NAPE-PLD activity Filomena Fezza, Nicolina Mastrangelo, and Mauro Maccarrone 19. A Fluorescence-based NAPE-PLD Activity Assay Elliot D. Mock, Wouter P. F. Driever, and Mario van der Stelt 20. Radiometric Assay of FAAH Activity Monica Bari, Monica Feole, Marina Fava, and Mauro Maccarrone 21. Fluorimetric Assay of FAAH activity Clotilde B. Angelucci, Roberto Giacominelli-Stuffler,and Mauro Maccarrone 22. Assay of NAAA Activity Kazuhito Tsuboi and Natsuo Ueda 23. Assay of DAGLa/ Activity Tiziana Bisogno 24. Assay of Monoacylglycerol Lipase Activity Kwang-Mook Jung and Daniele Piomelli 25. Radiometric Assay of ABHD2 Activity Domenico Fazio, Emanuele Criscuolo, and Mauro Maccarrone 26. Oxygenation of Anandamide by Lipoxygenases Guus van Zadelhoff and Mario van der Stelt 27. Assay of Endocannabinoid Oxidation by Cytochrome P450 Natasha T. Snider and Paul F. Hollenberg 28. Assays of Endocannabinoid Uptake Ines Reynoso-Moreno, Mark Rau, Andrea Chicca, Simon Nicolussi, and Jrg Gertsch 29. Assessing Gene Expression of the Endocannabinoid System Components by Real-Time Quantitative Reverse Transcription Polymerase Chain Reaction Mariangela Pucci and Claudio D’Addario 30. Bioinformatics of the Endocannabinoid System: Study of DNA methylation at Rat Cnr1 gene promoter Annalaura Sabatucci and Claudio D’Addario 31. DNA Methylation Analysis of Cnr1 Gene Promoter Claudio D’Addario and Mariangela Pucci 32. Western Blotting of the Endocannabinoid System Jim Wager-Miller and Ken Mackie 33. Quantitation of Plasma Membrane (G Protein-Coupled) Receptor Trafficking in Cultured Cells Jim Wager-Miller and Ken Mackie 34. Assessing CB1 Expression in the Brain by Immunohistochemical Methods: Light, Confocal and Electron Microscopy Roberta Imperatore and Luigia Cristino 35. Measuring Endocannabinoid System Interaction with Biomembranes Clotilde B. Angelucci, Annalaura Sabatucci, Ana Lia Bernardo, Alexandrine Kurtz, Sergio Oddi, and Enrico Dainese 36. STORM Super-Resolution Imaging of CB1 Receptors in Tissue Preparations Mikls Zldi and Istvn Katona 37. Visualization of Endocannabinoids in the Cell Sergio Oddi, Francesca Ciaramellano, Lucia Scipioni, Enrico Dainese, and Mauro Maccarrone 38. Electrophysiology of Endocannabinoid Signalling Alessandra Musella and Diego Centonze 39. Machine Learning and Computational Chemistry for the Endocannabinoid System Kenneth Atz, Wolfgang Guba, Uwe Grether, and Gisbert Schneider 40. A Virtual Reverse Screening Approach to Target Type 2 Cannabinoid Receptor Fulvio Ciriaco, Nicola Gambacorta, Francesco Leonetti, Cosimo Damiano Altomare,and Orazio Nicolotti
