Description
Transcriptomics and proteomics, studying the profile of the expression of nucleic acids and proteins respectively, are increasingly applied to gain a mechanistic insight into a wide spectrum of investigation, and the use of expression profiling studies for the central nervous system and brain function aids in the understanding of neurodegenerative disorders and tumor development mechanisms. In Expression Profiling in Neuroscience, expert researchers provide a survey of the most commonly used approaches in the field and scan the different ways of studying the central nervous system/brain environment through expression profiling. The first part addresses the gene expression profiling of the brain at a large scale or to a specific cell type such as blood-brain barrier endothelium. Then, the second part describes the protein expression studies and the different technologies applied. Written for the popular Neuromethods series, chapters include corresponding background information, tested laboratory protocols, and step-by-step methods for reproducible laboratory experiments. Detailed and authoritative, Expression Profiling in Neuroscience presents the state-of-the-art techniques necessary to expand research further into this vital area of study. 1. DNA Microarrays for Gene Expression Analysis in Brain Tissue and Cell Lines Gustavo A. Barisone and Elva Daz 2. Gene Expression Profiling Using the Terminal Continuation (TC) RNA Amplification Method for Small Input Samples in Neuroscience Stephen D. Ginsberg, Melissa J. Alldred, and Shaoli Che 3. Expression Profiling In Brain Disorders Peter J. Gebicke-Haerter 4. Endothelial Cell Heterogeneity of Blood-Brain Barrier Gene Expression: Analysis by LCM/qRT-PCR Tyler Demarest, Nivetha Murugesan, Jennifer Macdonald, and Joel S. Pachter 5. Gene Expression Profiling Using 3′ Digital Tag Approach Yan W. Asmann, E. Aubrey Thompson, and Jean-Pierre A. Kocher 6. Sharing Expression Profiling Data with Gemma Anton Zoubarev and Paul Pavlidis 7. Two-Dimensional Protein Analysis of Neural Stem Cells Martin H. Maurer 8. iTRAQ Proteomics Profiling of Regulatory Proteins During Oligodendrocyte Differentiation Mohit Raja Jain, Tong Liu, Teresa L. Wood, and Hong Li 9. Protein Profiling of the Brain: Proteomics of Isolated Tissues and Cells Nicole Haverland and Pawel Ciborowski 10. The Proteome of Brain Capillary Endothelial Cells: Towards a Molecular Characterization of an In Vitro Blood-Brain Barrier Model Sophie Duban-Deweer, Christophe Flahaut, and Yannis Karamanos 11. MALDI Imaging Technology Application in Neurosciences : From History to Perspectives Michel Salzet, Cline Mriaux, Julien Franck, Maxence Wistorski, and Isabelle Fournier 12. Profiling of HIV Proteins in Cerebrospinal Fluid Melinda Wojtkiewicz and Pawel Ciborowski 13. Proteomic Profiling of Cerebrospinal Fluid Gwenael Pottiez and Pawel Ciborowski 14. New Nanotechnology Applications in Single Cell Analysis: Why and How? Gradimir N. Misevic, Gerard BenAssayag, Bernard Rasser, Philippe Sales, Jovana Simic-Krstic, Nikola Misevic, and Octavian Popescu
