Description
This third edition volume expands on the previous editions both by presenting more detailed protocols for the techniques described in the first and second editions of High Throughput Screening: Methods and Protocols and by covering important new procedures. The first chapter of this book provides an overview of important assay development techniques, while the rest of the chapters detail how to develop and execute screens at whatever throughput the user needs. Some chapter examples are: structure-based virtual screening, high throughput screening using mass spectrometry, identification of state-dependent blockers for voltage gated calcium channels, bioluminescence resonance energy transfer platform to monitor protein-protein interactions in live cells, high throughput flow cytometry, and application of imaging-based assays in microplate formats for high content screening. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting edge and thorough, High Throughput Screening: Methods and Protocols, Third Edition, is a valuable resource for anyone who is interested in HTS research. 1. Design and Implementation of High Throughput Screening Assays David J. Powell, Robert P. Hertsberg, and Ricardo Macarrn 2. Characterization of Inhibitor Binding Through Multiple Inhibitor Analysis: A Novel Local Fitting Method Thomas V. Riera, Tim J. Wigle, and Robert A. Copeland 3. High Throughput Screening Using Mass Spectrometry within Drug Discovery Mattias Rohman and Jonathan Wingfield 4. Structure-Based Virtual Screening of Commercially Available Compound Libraries Dmitri Kireev 5. AlphaScreen-Based Assays: Ultra-High Throughput Screening for Small Molecule Inhibitors of Challenging Enzymes and Protein-Protein Interactions Adam Yasgar, Ajit Jadhav, Anton Simeonov, and Nathan P. Coussens 6. Instrument Quality Control Chatura Jayakody and Emily A. Hull-Ryde 7. Application of Fluorescence Polarization in HTS Assaysn: NanoLuc-Based Bioluminescence Resonance Energy Transfer Platform to Monitor Protein-Protein Interactions in Live Cells Xiu-Lei Mo and Haian Fu 18. Application of Imaging-Based Assays in Microplate Formats for High Content Screening Adam I. Fogel, Scott E. Martin, and Samuel A. Hasson
