Description
The volume aims to explore the dynamic nature of the autophagy pathway, and the latest techniques that allow researchers to capture and quantify this process in neurons. The chapters in this volume cover topics such as fundamental, historical, and functional approaches that began in baker’s yeast; Saccharomyces cerevisiae; the role of both electron microscopy and live-cell imaging using fluorescently tagged autophagy proteins; and the rate of puncta appearance and its correlation with the rate of autophagosome formation. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and practical, Imaging and Quantifying Neuronal is a valuable resource that provides insights into the power of microscopy tools, live-cell imaging, and photoactivation and correlative techniques. Preface to Series… Preface… Table of Contents… Contributing Authors… 1. Quantification of Autophagosome Size and Formation Rate by Electron and Fluorescence Microscopy in Baker’s Yeast Steven K. Backues and Daniel J. Klionsky 2. Ultrastructure of the Macroautophagy Pathway in Mammalian Cells Eeva-Liisa Eskelinen and Katri Kallio 3. Live Imaging of Autophagosome Biogenesis and Maturation in Primary Neurons Andrea K. H. Stavoe and Erika L. F. Holzbaur 4. Monitoring Autophagic Activity In Vitro and In Vivo using the GFP-LC3-RFP-LC3G Probe Tomoya Eguchi, Hideaki Morishita, and Noboru Mizushima 5. Measuring Autophagic Flux in Neurons by Optical Pulse Labeling Nicholas A. Castello and Steven Finkbeiner 6. Measuring Autophagosome Flux Andre du Toit, Jan-Hendrik S. Hofmeyr, and Ben Loos 7. Measurement of Neuronal Tau Clearance In Vivo Ana Lopez, Angeleen Fleming, and David C. Rubinsztein 8. Methods for Studying Axonal Autophagosome Dynamics in Adult Dorsal Root Ganglion Neurons Xiu-Tang Cheng, Kelly A. Chamberlain, and Zu-Hang Sheng 9. Imaging and Quantifying Neuronal Autophagy to Determine the Autophagy Contribution to Neuronal and Dendritic Morphogenesis Wan Yun Ho and Shuo-Chien Ling 10. Correlative Light and Electron Microscopy (CLEM): Bringing Together the Best of Both Worlds to Study Neuronal Autophagy Jurgen Kriel, Dumisile Lumkwana, Lydia-Marie Joubert, Martin L. Jones, Christopher J. Peddie, Lucy Collinson, Ben Loos, and Lize Engelbrecht Subject Index List…
