Description
This detailed volume explores key technologies that are used currently to investigate iGluR structure, function and physiology. Chapters in this book cover methods to help illuminate the assembly, trafficking, molecular composition and subcellular location of iGluRs; approaches used to understand the atomic organization of iGluRs; and techniques to monitor receptor activity in real time. Written in the popular Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your own laboratory. Concise and easy-to-use, Ionotropic Glutamate Receptor Technologies aims to facilitate the implementation of specific methods to iGluR investigations. Part I: Protein Assembly and Trafficking 1. Assaying AMPA Receptor Oligomerization Catherine L. Salussolia, Quan Gan, and Lonnie P. Wollmuth 2. A Step-by-Step Guide to Single Subunit Counting Of Membrane-Bound Proteins in Mammalian Cells Mark R. P. Aurousseau, Hugo McGuire, Rikard Blunck, and Derek Bowie 3. Counting NMDA Receptors at the Cell Surface Martin Horak and Young Ho Suh 4. Electron Microscopy Analysis of AMPA receptors in Dendritic Spines Audra A. Kramer, Amber N. Petersen, and Nashaat Z. Gerges 5. Electrophysiological Tagging of Ionotropic Receptors Andres Barria 6. Functional Detection of Novel Triheteromeric NMDA Receptors Sanjay S. Kumar Part II: Protein Structure and Dynamics 7. Calcium Imaging to Study NMDA Receptor-mediated Cellular Responses Kelly A. Krogh and Stanley A. Thayer 8. NMR Approaches to Functional Dynamics Of Genetically Separated iGluR Domains Christopher P. Ptak, Ahmed H. Ahmed, and Robert E. Oswald 9. Computing Conformational Free Energies of iGluR Ligand-Binding Domains Alvin Yu, Tyler Wied, John Belcher, and Albert Y. Lau 10. LRET Methods for Investigating Conformational Changes in Functional Ionotropic Glutamate Receptors Rita E. Sirrieh and Vasanthi Jayaraman 11. Assaying the Energetics of NMDA Receptor Pore Opening Rashek Kazi, Melissa Daniel, and Lonnie P. Wollmuth Part III: Protein Function 12. Constructing a Rapid Solution Exchange System David M. MacLean 13. Assessing Effects of Ligand-Binding Mutations To AMPA And Kainate Receptor Kinetics Mark W. Fleck 14. Analysis of Whole-Cell NMDA Receptor Responses Vojtech Vyklicky, Miloslav Korinek, Ales Balik, Tereza Smejkalova, Barbora Krausova, and Ladislav Vyklicky 15. Expression, Purification, and Crystallization of Full Length Ionotropic Glutamate Receptors Maria V. Yelshanskaya, Kei Saotome, Minfen Li, and Alexander I. Sobolevsky 16. Timing AMPA Receptor Activation with Laser-pulse Photolysis Li Niu 17. Current Recording and Kinetic Analyses for Single AMPA Receptors Kinning Poon, Robert E. Oswald, and Linda M. Nowak 18. Extracting Rate Constants for NMDA Receptor Gating from One-channel Current Recordings Kirstie A. Cummings, Gary J. Iacobucci, Gabriela K. Popescu
