Description
Part I: Introductory Reviews 1. Introduction and Classification of Leukemias Geoffrey Brown 2. Leukemia Stem Cells: Concept and Implications Isidro Snchez-Garca and Csar Cobaleda 3. Leukemia Stem Cell Drug Discovery Csar Cobaleda and Isidro Snchez-Garca Part II: Protocols 4. Analysis and Isolation of Mouse Leukemic Stem Cells Fang Dong, Haitao Bai, and Hideo Ema 5. Mass Cytometry of Hematopoietic Cells Astraea Jager, Jolanda Sarno, and Kara Davis 6. PCR Technology to Identify Minimal Residual Disease Giovanni Cazzaniga, Simona Songia, and Andrea Biondi, on behalf of the EuroMRD Working Group 7. Next-Generation Sequencing Technology to Identify Minimal Residual Disease in Lymphoid Malignancies Michaela Kotrova, Nikos Darzentas, Christiane Pott, and Monika Brggemann, on behalf of the EuroClonality-NGS Working Group 8. Genomic Inverse PCR for Screening of Preleukemic Cells in Newborns (GIPFEL Technology) Daniel Hein, Arndt Borkhardt, and Ute Fischer 9. Single-Cell Transcriptomic Analysis of Hematopoietic Cells Paulina M. Strzelecka, Anna M. Ranzoni, and Ana Cvejic 10. In-Depth Mass Spectrometry-Based Single-Cell and Nanoscale Proteomics Yiran Liang, Thy Truong, Ying Zhu, and Ryan T. Kelly 11. In Vivo Clonal Analysis of Aged Hematopoietic Stem Cells: Single-Cell Transplantation Kyomi J. Igarashi and Ryo Yamamoto 12. Experimental Competitive Bone Marrow Transplant Assays Roxann Htu-Arbour, Sarah Bouali, and Krista M. Heinonen 13. Human T-ALL Xenografts Patricia Fuentes, Mara L. Toribio, and Sara Gonzlez-Garca 14. An Experimental and Computational Protocol to Study Cell Proliferation in Human Acute Myeloid Leukemia Xenografts Thalia Vlachou, Marco S. Nobile, Chiara Ronchini, Daniela Besozzi, and Pier Giuseppe Pelicci 15. Leukemic Stem Cell Culture in Cytokine-Free Medium Xiaolei Liu and Peter S. Klein 16. Ex Vivo Expansion of Adult Hematopoietic Stem and Progenitor Cells with Valproic Acid Luena Papa, Mansour Djedaini, Manisha Kintali, Christoph Schaniel, and Ronald Hoffman 17. Isolation, Culture, and Manipulation of Human Cord Blood Progenitors Cristina Prieto, Damia Romero-Moya, and Rosa Montes 18. Lentiviral Transduction for Optimal LSC/HSC Manipulation Gustavo Mostoslavsky 19. Characterizing the In Vivo Role of Candidate Leukemia Stem Cell Genes Yu Wei Zhang, Julian Mess, and Nina Cabezas-Wallscheid 20. Clonal Analysis of Patient-Derived Samples Using Cellular Barcodes Sabrina Jacobs, Leonid V. Bystrykh, and Mirjam E. Belderbos 21. Arrayed Molecular Barcoding of Leukemic Stem Cells Marion Chapellier and Marcus Jrs 22. In Vivo Generation of Leukemic Stem Cells by HSC Targeting by Transgenesis Carolina Vicente-Dueas 23. In Situ Hematopoietic Stem Cell Imaging Aparna Venkatraman, Sarah E. Smith, Sandra Pinho, Meng Zhao, Linheng Li, and Paul Frenette 24. A Genome-Editing System for Therapeutical Targeting of Stem Cells Giacomo Frati and Annarita Miccio 25. Method for the Generation of Induced Hematopoietic Stem Cells Leonid Olender, Klil Levy, and Roi Gazit 26. Modeling Leukemia Stem Cells with Patient-Derived Induced Pluripotent Stem Cells Andr G. Deslauriers, Andriana G. Kotini, and Eirini P. Papapetrou 27. High Content Imaging to Phenotype Human Primary and iPSC-Derived Cells Lorenzo Veschini, Heba Sailem, Disha Malani, Vilja Pietiinen, Ana Stojiljkovic, Erika Wiseman, and Davide Danovi 28. Bioinformatic Methods to Identify Mutational Signatures in Cancer S.M. Ashiqul Islam and Ludmil B. Alexandrov
