Description
This second edition volume expands on the previous edition by discussing classic techniques, as well as new protocols that focus on the preparation of liposomes, lipid characterization, particle size and charge analysis, drug encapsulation, surface modification, stimuli response, and cellular interaction and biodistribution. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, Liposomes: Methods and Protocols, Second Edition is a valuable resource for graduate students, post-doctoral researchers, and established investigators utilizing lipid-based systems in the fields of cell and molecular biology, drug delivery, and physical chemistry. Preface… Table of Contents… Contributing Authors… 1. Liposomes Came First: The Early History of Liposomology Volkmar Weissig 2. Thin Film Hydration followed by Extrusion Method for Liposome Preparation Hongwei Zhang 3. Preparation of DRV Liposomes Sophia G. Antimisiaris 4. Method of Simultaneous Analysis of Liposome Components by HPTLC/FID Sophia Hatziantoniou and Costas Demetzos 5. Freeze-Fracture Electron Microscopy on Domains in Lipid Mono- and Bilayer on Nano-Resolution Scale Brigitte Papahadjopoulos-Sternberg 6. Liposome Formulations of Hydrophobic Drugs Reto A. Schwendener and Herbert Schott 7. A “Dock and Lock” Approach to Preparation of Targeted Liposomes Marina V. Backer and Joseph M. Backer 8. Coupling of Ligands to the Liposome Surface by Click Chemistry Maria Vittoria Spanedda, Marcella De Giorgi, Fatouma Sad Hassane, Francis Schuber, Line Bourel-Bonnet, and Benot Frisch 9. Elastic Liposomes for Topical and Transdermal Drug Delivery
