Description
This volume focuses on malaria vaccinology, and introduces researchers to a complete overview of the protocols and tools used by molecular and cellular malariologists. The chapters within this book talk about interventions concerning all aspects of life cycle progression–measuring antibody responses to blood stage parasite survival, the T Cell responses engendered by attenuated sporozoite vaccinations, and the unique effect on transmission of antibodies that target the mosquito stage of the life cycle. Additionally, methods concerning the ability to generate targeted gene deletions and replacements in the genome of Plasmodium parasites convey how Plasmodium parasite phenotypes can be created to precise specifications. More recently, the potential power of humanized mouse models of disease progression have been demonstrated and are also discussed in the book. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Malaria Vaccines: Methods and Protocols is a useful and unique resource for any aspiring malaria vaccinologists because of the volume’s exquisitely detailed methodologies that are not typically found in published literature. Ashley Vaughan, Ph.D. Senior Scientist Seattle Biomedical Research Institute (Seattle BioMed) 307 Westlake Avenue N, Suite 500Seattle, WA 98109 Phone: (206) 256-7115 E-mail: ashley.vaughan@seattlebiomed.org Part I Pre-Erythrocytic Stages 1. Isolation of Non-Parenchymal Cells from the Mouse Liver Isaac Mohar, Katherine J. Brempelis, Sara A. Murray, Mohammad R. Ebrahimkhani, and I. Nicholas Crispe 2. Measurement of the T Cell Response to Pre-Erythrocytic Vaccination in Mice Jenna J. Guthmiller, Ryan A. Zander, and Noah S. Butler 3. Characterization of Liver CD8 T cell Subsets that are Associated with Protection against Pre-Erythrocytic Plasmodium Parasites Stasya Zarling and Urszula Krzych 4. Flow Cytometry-Based Assessment of Antibody Function against Malaria Pre-Erythrocytic Infection Alyse N. Douglass, Peter G. Metzger, Stefan H.I. Kappe, and Alexis Kaushansky 5. Assessment of Parasite Liver Stage Burden in Human-Liver Chimeric Mice Lander Foquet, Philip Meuleman, Rob Hermsen, Robert Sauerwein, and Geert Leroux-Roels 6. Measurement of Antibody-Mediated Reduction of Plasmodium Yoelii Liver Burden by Bioluminescent Imaging Brandon K. Sack, Jessica L. Miller, Ashley M. Vaughan and Stefan H. I. Kappe 7. Detection of Plasmodium Berghei and Plasmodium Yoelii Liver-Stage Parasite Burden by Quantitative Real Time PCR Alexander Pichugin and Urszula Krzych Part II Mosquito Stages 8. Membrane Feeding Assay to Determine the Infectiousness of Plasmodium Vivax Gametocytes Jetsumon Sattabongkot Prachumsri, Chalermpon Kumpitak, and Kirakorn Kiattibutr 9. The Standard Membrane Feeding Assay: Advances Using Bioluminescence Will J. R. Stone and Teun Bousema Part III Erythrocytic Stages 10. Agglutination Assays of the Plasmodium Falciparum Infected Erythrocyte Joshua Tan and Peter C. Bull 11. Antibody-Dependent Cell-Mediated Inhibition (ADCI) of Plasmodium Falciparum: One and Two-Step ADCI Assays Hasnaa Bouharoun-Tayoun and Pierre Druilhe 12. A Robust Phagocytosis Assay to Evaluate the Opsonic Activity of Antibodies against Plasmodium Falciparum-Infected Erythrocytes Andrew Teo, Wina Hasang, Philippe Boeuf, and Stephen Rogerson 13. Miniaturized Growth Inhibition Assay to Assess the Anti-Blood Stage Activity of Antibodies Elizabeth H. Duncan and Elke S. Bergmann-Leitner 14. Measuring Plasmodium Falciparum Erythrocyte Invasion Phenotypes Using Flow Cytometry Amy Kristine Bei and Manoj T. Duraisingh 15. The In Vitro Invasion Inhibition Assay (IIA) for Plasmodium Vivax Wanlapa Roobsoong 16. The Ex Vivo IFN- Enzyme-Linked Immunospot (ELISpot) Assay Martha Sedegah 17. Evaluating IgG Antibody to Variant Surface Antigens Expressed on Plasmodium Falciparum Infected Erythrocytes Using Flow Cytometry Andrew Teo, Wina Hasang and Stephen Rogerson 18. Inhibition of Infected Red Blood Cell Binding to the Vascular Endothelium Marion Avril 19. Evaluation of Pregnancy Ma laria V accine Candidates: The Binding Inhibition Assay Tracy Saveria, Patrick E. Duffy, and Michal Fried 20. High-Throughput Testing of Antibody Dependent Binding Inhibition of Placental Malaria Parasites Morten A. Nielsen and Ali Salanti Part IV Parasite Manipulation 21. Generation of Transgenic Rodent Malaria Parasites Expressing Human-Malaria Parasite Proteins Ahmed M. Salman, Catherin Marin Mogollon, Jing-wen Lin, Fiona J.A. van Pul, Chris J. Janse, and Shahid M. Khan Part V Vaccination 22. Vaccination Using Gene-Gun Technology Elke Bergmann-Leitner and Wolfgang W. Leitner
