Description
A cutting-edge collection of readily reproducible in vitro and in vivo methods to elucidate the mechanisms associated with cannabinoid function in health and disease. The techniques can be used in studies across the board from genes to behavior. The molecular neurobiological methods are invaluable in analyzing the structure, the polymorphisms, and the molecular expression of the cannabinoid receptors (CBrs), as well as their association with polysubstance abuse. There are also methods for localizing cannabinoid receptors in different systems, visualizing cannabinoid effects using brain slice imaging and elctrophysiological approaches, and designing and synthesizing cannabinoids and endocannabinoids. The protocols follow the successful Methods in Molecular Medicine(TM) series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Molecular Neurobiological Methods in Marijuana-Cannabinoid Research George R. Uhl, Hiroki Ishiguro, Emmanuel S. Onaivi, Zhicheng Lin, B. Emmanuel Akinshola, Bruce Hope, Claire M. Leonard, and Qing-Rong Liu Experimental Methods to Study the Role of the Peripheral Cannabinoid Receptor in Immune Function Nancy E. Buckley, Diep Burbridge, Manop Buranapramest, Tanya Ferguson, and Renee Y. Paau Localization of Cannabinoid Receptors Using Immunoperoxidase Methods Guy A. Cabral Localization of Cannabinoid CB1 Receptor mRNA Using Ribonucleotide Probes: Methods for Double- and Single-Label In Situ Hybridization Andrea G. Hohmann Morphometric Study on Cytoskeletal Components of Neuronal and Astroglial Cells After a Chronic CB1 Agonist Treatment Patricia Tagliaferro, Alberto J. Ramos, Emmanuel S. Onaivi, Sergio G. Evrard, Maite Duhalde Vega, and Alicia Brusco Visualizing Cannabinoid Effects Using Brain Slice Imaging and Electrophysiological Approaches Alexander F. Hoffman and Carl R. Lupica Methods for the Synthesis of Cannabinergic Ligands Ganesh A. Thakur, Spyros P. Nikas, Richard I. Duclos, Jr., and Alexandros Makriyannis Cannabinoid Receptor Binding to Membrane Homogenates and Cannabinoid-Stimulated [35S]GTPgS Binding to Membrane Homogenates or Intact Cultured Cells Chris Breivogel Methods to Assay Anandamide Hydrolysis and Transport in Synaptosomes Filomena Fezza, Natalia Battista, Monica Bari, and Mauro Maccarrone Methods Evaluating Cannabinoid and Endocannabinoid Effects on Gastrointestinal Functions Nissar A. Darmani The Bioassay of Cannabinoids Using the Mouse Isolated Vas Deferens Ad`ele Thomas and Roger G. Pertwee Assessment of Cannabis Craving Using the Marijuana Craving Questionnaire Stephen J. Heishman and Edward G. Singleton Methods for Studying Acute and Chronic Effects ofMarijuana on Human Associative Processes and Memory Robert I. Block Methods for Clinical Research Involving Cannabis Administration David A. Gorelick and Stephen J. Heishman Neurological Assessments of Marijuana Users Jean Lud Cadet, Karen Bolla, and Ronald I. Herning Behavioral Methods in Cannabinoid Research Ester Fride, Alex Perchuk, F. Scott Hall, George R. Uhl, and Emmanuel S. Onaivi Methods to Study the Behavioral Effects and Expression of CB2 Cannabinoid Receptors and Its Gene Transcripts in Chronic Mild Stress Model of Depression Emmanuel S. Onaivi, Hiroki Ishiguro, Patel Sejal, Paul A. Meozzi, Lester Myers, Patricia Tagliaferro, Bruce Hope, Claire M. Leonard, George R. Uhl, Alicia Brusco, and Eileen Gardner Index
