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Preface… Table of Contents… Contributing Authors… Part I Methods for the Detection of Melatonin and Its Biological Derivatives 1. Two-Dimensional Thin Layer Chromatography of Melatonin and Related Compounds David C. Klein 2. Detection of Melatonin in Tissue Samples Cline Legros and Jean A. Boutin 3. Measuring Dim Light Melatonin Onset in Humans Melissa A. St. Hilaire and Steven W. Lockley 4. Measuring Urinary 6-sulphatoxymelatonin in Humans Melissa A. St. Hilaire and Steven W. Lockley 5. MEL-Index: Estimation of Tissue Melatonin Levels using Gene Expression Data Pedro Augusto CM Fernandes, Regina P. Markus, and Gabriela Sarti Kinker 6. Melatonin Synthesis Enzymes Activity: Radiometric Assays for AANAT, ASMT, and TPH Fernanda Gaspar do Amaral, Jos Cipolla-Neto, and Solange Castro Afeche 7. Pinealectomy in Rats Camila Congentino Gallo, Fernanda Akane Nishino, Fernanda Gaspar do Amaral, and Jos Cipolla-Neto 8. Surgical Techniques and Nuances for Superior Cervical Ganglionectomy and Decentralization in Rats Sarosh Irfan Madhani, David C. Klein, Estela M. Muoz, and Luis E. Savastano 9. Pineal Microdialysis Caroline Aparecida Pereira de Souza, Fernanda Akane Nishino, Fernanda Gaspar do Amaral, and Jos Cipolla-Neto 10. Radiochemical In Situ Hybridization in Developmental Studies of the Pineal Gland Martin Fredensborg Rath and Morten Mller 11. Pineal Cells Dissociation and Culture: Isolated Pinealocytes, Isolated Astrocytes, and Co-Culture Solange Castro Afeche, Diego de Piazza Pimentel, Lus Felipe Ferro, and Jos Cipolla-Neto 12. Pineal Gland Culture Solange Castro Afeche, Fernanda Gaspar do Amaral, and Jos Cipolla-Neto 13. Preparation of Single Pineal Cells Steven L. Coon and David C. Klein 14. RNA Sequencing of Single Pineal CellsSteven L. Coon and David C. Klein 15. siRNA-Mediated Downregulation of Gene Expression in Cultured Rat Pineal Cells Aurea Susana Blancas-Velazquez and Martin Fredensborg Rath 16. Analysis of the Human Pineal Proteome by Mass Spectrometry Mariette Matondo, Guillaume Dumas, and Erik Maronde 17. The Pineal Transcriptome Webpage David C. Klein, Steven L. Coon, Carol Ensault, and Dale Ryan Part II Methods to Measure Melatonin Effects at the Molecular and Cellular Level 18. 2-[125I]iodomelatonin and [3H]melatonin Binding Assays for Melatonin Receptors Florence Gbahou and Ralf Jockers 19. Alternative Ligands at Melatonin Receptors Cline Legros, Said Yous, and Jean A. Boutin 20. GTPS Binding Assay for Melatonin Receptors in Mouse Brain Tissue Romain Gerbier and Ralf Jockers 21. MT1 Melatonin Receptor Reconstitution in Nanodiscs Jean A. Boutin, Christel Logez, Marjorie Damian, Renaud Wagner, Jean-Louis Banres, and Gilles Ferry 22. Functional Investigation of Melatonin Receptors Activation by Homogenous cAMP Assay Erika Cecon, Jean-Luc Guillaume, and Ralf Jockers 23. Functionality of Melatonin Receptors: Internalization Clmence Dupr, Cline Legros, and Jean A. Boutin 24. Functionality of Melatonin Receptors: Recruitment of -arrestin at MT1 Clmence Dupr, Cline Legros, and Jean A. Boutin 25. MT1 Receptor Signaling Pathways by Impedance Measurement Anne Bonnaud, Clmence Dupr, Cline Legros, and Jean A. Boutin 26. Measuring Protein-Protein Interactions of Melatonin Receptors by Bioluminescence Resonance Energy Transfer (BRET) Atsuro Oishi and Ralf Jockers 27. Non-Radioactive In Situ Hybridization of MT1 Melatonin Receptor for the Identification of Melatonin Target Cells Paul Klosen 28. Beta-Galactosidase as a Transgenic Reporter for the Mapping and Phenotyping of MT1 and MT2 Melatonin Receptor Expressing Cells Paul Klosen 29. Why Are We Still Cloning Melatonin Receptors? A Commentary Clia Gautier, Isabelle Theret, Giulia Lizzo, Gilles Ferry, Sophie-Pnlope Gunin, and Jean A. Boutin 30. Measuring Binding at the Putative Melatonin Receptor MT3 Cline Legros, Philippe Dupuis, Gilles Ferry, and Jean A. Boutin 31. Cloning, Expression, Purification, Crystallization, and X-Ray Structural Determination of the Human NQO2 in Complex with Melatonin Barbara Calamini, Gilles Ferry, and Jean A. Boutin 32. Melatonin Binding to Human NQO2 by Isothermal Titration Calorimetry Barbara Calamini, Gilles Ferry, and Jean A. Boutin 33. Measurement of NQO2 Catalytic Activity and of Its Inhibition by Melatonin Gilles Ferry and Jean A. Boutin 34. Measuring the NQO2: Melatonin Complex by Native Nano-Electrospray Ionization Mass Spectrometry Jean A. Boutin, Johann Stojko, Gilles Ferry, and Sarah Cianferani 35. Human and Rodent Cell Lines as Models of Functional Melatonin-Responsive Pancreatic Islet Cells Juliane Zibolka, Ina Bhr, Elmar Peschke, Eckhard Mhlbauer, and Ivonne Bazwinsky-Wutschke 36. Mesenchymal Stem Cell and Monocyte Co-Cultures Fahima Munmun and Paula A. Witt-Enderby 37. Real-Time Monitoring of Circadian Rhythms in the Eye Kenkichi Baba and Gianluca Tosini Part III Methods to Measure Melatonin Effects In Vivo 38. Assessing the Role of Melatonin in the Modulation of Visual Functions in the Mouse Kenkichi Baba and Gianluca Tosini 39. Methods to Assess Melatonin-Receptor Mediated Phase-Shift and Re-Entrainment of Rhythmic Behaviors in Mouse Models Grant C. Glatfelter, Jennifer Sosa, Randall L. Hudson, and Margarita L. Dubocovich 40. Neurotrasmission Recovery by Melatonin Measured by CMAP Samuele Negro, Marco Stazi, Michela Rigoni, and Aram Megighian 41. Olfaction and Melatonin: The Use of the Olfactory Discrimination Test Ana Carolina Duarte Noseda and Marcelo M. S. Lima 42. Photothrombotic Mouse Models for the Study of Melatonin as a Therapeutic Tool After Ischemic Stroke Marco Cambiaghi, Laura Cherchi, and Stefano Comai 43. Hypnotic Effects of Melatonergic Compounds Measured in Mice or Rats Stefano Comai and Danilo De Gregorio 44. Acute and Chronic Pain Preclinical Models to Study the Analgesic Properties of Melatonergic Compounds Danilo De Gregorio and Stefano Comai 45. Behavioral and Fluorescent-Based Immunohistochemistry Protocols for Examining Anti-Depressant-Like Effects of Melatonin in Mice Estrada-Reyes Rosa, Muoz Estrada Jess, and Bentez-King Gloria 46. A Method for Perfusion of Tissue-Isolated Human Tumor Xenografts in Nude Rats to Investigate the Oncostatic Role of the Physiological Nocturnal Melatonin Signal Robert T. Dauchy, Steven M. Hill, and David E. Blask 47. A Method for Growing Tissue-Isolated Human Tumor Xenografts in Nude Rats for Melatonin/Cancer Studies Robert T. Dauchy, Steven M. Hill, and David E. Blask Subject Index List…
