Description
Part I Cellular Analyses of Neural Progenitors 1. Dissociation of Cerebellar Granule Neuron Progenitors for Culture, FACS, Transcriptomics, and Molecular Biology Jennifer Karin Ocasio 2. Maintaining Cerebellar Granule Neuron Progenitors in Cell Culture Jennifer Karin Ocasio 3. Proliferation Analysis of Cerebellar Granule Neuron Progenitors for Microcephaly Research, Using Immunofluorescent Staining and Flow Cytometry Jennifer Karin Ocasio 4. Conventional and Spectral Karyotyping of Murine Cerebellar Granule Neuron Progenitors Gouri Nanjangud Part II Analyses of Neural Progenitors In Situ 5. Processing of Neonatal Mouse Brains for Immunohistochemical Analysis, As Required for Including Spindle Orientation Analysis and Analysis of DNA Damage and Apoptosis Gabriela De la Cruz, Nana Nikolaishvili-Feinberg, Scott E. Williams, and Timothy R. Gershon 6. Automated Immunofluorescence Staining for Analysis of DNA Damage and Apoptosis in Brain Sections Gabriela De la Cruz, Nana Nikolaishvili-Feinberg, and Timothy R. Gershon 7. Automated Immunofluorescence Staining for Analysis of Mitotic Stages and Division Orientation in Brain Sections Gabriela De la Cruz, Nana Nikolaishvili-Feinberg, and Scott E. Williams Part III RNA-seq Analysis with Single-Cell Resolution (scRNA-seq) 8. scRNA-seq for Microcephaly Research [1]: Single-Cell Droplet Encapsulation, mRNA Capture, and cDNA Synthesis Benjamin Babcock and Seth Weir 9. scRNA-seq for Microcephaly Research [2]: Preparation of Single-Cell cDNA Libraries Benjamin Babcock and Seth Weir 10. scRNA-seq for Microcephaly Research [3]: Computational Analysis of scRNA-seq Data Benjamin Babcock and Daniel Malawsky 11. scRNA-seq for Microcephaly Research [4]: Dirichlet Regression for Single Cell Population Differences Daniel Malawsky and Timothy R. Gershon Part IV MRI Analyses of the Mouse Brain 12. Mouse Brain MRI, Including In Vivo, Ex Vivo, and fcMRI for the Study of Microcephaly Martin J. MacKinnon, Tzu-Wen W. Wang, and Yen-Yu I. Shih 13. Whole-Body Mouse Fluxomic Analysis to Detect Metabolic Disruptions Associated with Microcephaly, using 13C Isotopes Andrey P. Tikunov Part V Behavioral Phenotyping in Neonatal Mice 14. Neonatal Behavioral Screen for Mouse Models of Neurodevelopmental Disorders Kathryn M. Harper, Viktoriya D. Nikolova, Monika E. Conrad, and Sheryl S. Moy
