Description
Preface List of Abbreviations Part 1: Introduction 1.1 Background 1.2 Aim, Scope and Purpose of the Book 1.3 Organization of the Book 1.4 Terminology 1.5 Regulatory Compliance 1.6 References Part 2: General Considerations 2.1 Clinical Information 2.2 Basic Principles of Molecular Analysis 2.3 Troubleshooting 2.4 References Part 3: Protocols 3.1 Sample Shipment and Storage 3.2 Reagents 3.3 Harvesting WBCs by RBC Sedimentation 3.4 Short-term Cell Cultures from Amniotic Fluid 3.5 Cultured Amniocyte Cell Harvesting 3.6 Cell Preparation and DNA Extraction 3.7 Assessment of DNA Quality, Quantity and Purity 3.8 PCR Amplification 3.9 Restriction Enzyme Digestion for PCR-RFLP Analysis 3.10 Allele-specific PCR 3.11 Agarose Gel Preparation and Electrophoresis 3.12 Acrylamide Gel Preparation and Electrophoresis 3.13 Single-strand Conformation Polymorphism (SSCP) 3.14 Optimizing a PCR 3.15 Gene Zygosity Assessment by Quantitative PCR Amplification 3.16 VNTR and STR Analysis by PCR Amplification 3.17 Multiplex PCR Analysis 3.18 Southern Analysis 3.19 References Part 4: Red Cell Blood Groups 4.1 Terminology 4.2 Clinical Applications 4.3 Situations where Genotype and Phenotype do not Agree 4.4 References 4.5 Facts Sheets, Gene Maps and Molecular Protocols Part 5: Platelet Blood Groups 5.1 Terminology 5.2 Clinical Applications 5.3 References 5.4 Facts Sheets, Gene Maps and Molecular Protocols Part 6: Neutrophil Blood Groups 6.1 Terminology 6.2 Clinical Applications 6.3 References 6.4 Facts Sheets, Gene Maps and Molecular Protocols Part 7: Appendices 7.1 Database Information 7.2 Glossary 7.3 References 7.4 Works Sheets and Request Forms Index Useful Tables
