Description
This detailed book arrives as there is an increasing need for multiplex biomarker readouts for improved clinical management and to support the development of new drugs by pharmaceutical companies, due to continuous technical developments and new insights into the high complexity of many diseases. Chapters explore the basic technology platforms being applied in the fields of genomics, proteomics, transcriptomics, metabolomics, and imaging, which are currently the methods of choice in multiplex biomarker research. The book also describes the chief medical areas in which the greatest progress has been made and highlight areas where further resources are required. Written for the highly successful Methods in Molecular Biology series, methodology chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Part I: Reviews 1. Application of Multiplex Biomarker Approaches to Accelerate Drug Discovery and Development Hassan Rahmoune and Paul C. Guest 2. The Application of Multiplex Biomarker Techniques for Improved Stratification and Treatment of Schizophrenia Patients Johann Steiner, Paul C. Guest, Hassan Rahmoune, and Daniel Martins-de-Souza 3. Multiplex Biomarker Approaches in Type 2 Diabetes Mellitus Research Susan E. Ozanne, Hassan Rahmoune, Daniel Martins-de Souza, and Paul C. Guest 4. LC-MSE, Multiplex MS/MS, Ion Mobility, and Label-Free Quantitation in Clinical Proteomics Gustavo Henrique Martins Ferreira Souza, Paul C. Guest, and Daniel Martins-de-Souza 5. Phenotyping Multiple Subsets of Immune Cells In Situ in FFPE Tissue Sections: An Overview of Methodologies James R. Mansfield Part II: Statistical Considerations 6. Identification and Clinical Translation of Biomarker Signatures: Statistical Considerations Emanuel Schwarz 7. Opportunities and Challenges of Multiplex Assays: A Machine Learning Perspective Junfang Chen and Emanuel Schwarz Part III: Protocols 8. Multiplex Analyses Using Real-Time Quantitative PCR Steve F.C. Hawkins and Paul C. Guest 9. Multiplex Analysis Using cDNA Transcriptomic Profiling Steve F.C. Hawkins and Paul C. Guest 10. Multiplex Single Nucleotide Polymorphism Analyses Steve F.C. Hawkins and Paul C. Guest 11. Pulsed SILAC as a Proteomic Approach for miRNA Targets Identification in Cell Culture Daniella E. Duque-Guimares, Juliana de Almeida-Faria, Thomas Prates Ong, and Susan E. Ozanne 12. Blood Bio-Sampling Procedures for Multiplex Biomarkers Studies Paul C. Guest and Hassan Rahmoune 13. Multiplex Immunoassay Profiling Laurie Stephen 14. Multiplex Sequential Immunopreciptation of Insulin Secretory Granule Proteins from Radiolabeled Pancreatic Islets Paul C. Guest 15. Two Dimensional Gel Electrophoresis of Insulin Secretory Granule Proteins from Biosynthetically-Labeled Pancreatic Islets Paul C. Guest 16. Depletion of Highly Abundant Proteins of the Human Blood Plasma: Applications in Proteomics Studies of Psychiatric Disorders Sheila Garcia, Paulo A. Baldasso, Paul C. Guest, and Daniel Martins-de-Souza 17. Simultaneous Two-Dimensional Difference Gel Electrophoresis (2D-DIGE) Analysis of Three Distinct Proteomes Adriano Aquino, Paul C. Guest, and Daniel Martins-de Souza 18. Selective Reaction Monitoring for Quantitation of Cellular Proteins Vitor M. Faa 19. Characterization of a Protein Interactome by Co-Immunoprecipitation and Shotgun Mass Spectrometry Giuseppina Maccarrone, Juan Jose Bonfiglio, Susana Silberstein, Christoph W. Turck, and Daniel Martins-de-Souza 20. Using 15N-Metabolic Labeling for Quantitative Proteomic Analyses Maccarrone Giuseppina, Alon Chen, and Michaela D. Filiou 21. Multiplex Measurement of Serum Folate Vitamers by UPLC-MS/MS Sarah Meadows 22. UPLC-MS/MS Determination of Deuterated 25-Hydroxyvitamin D (d3-25OHD3) and Other Vitamin D Metabolites for the Measurement of 25OHD Half-Life Shima Assar, Inez Schoenmakers, Albert Koulman, Ann Prentice, and Kerry S. Jones 23. iTRAQ-Based Shotgun Proteomics Approach for Relative Protein Quantification Erika Velsquez Nez, Gilberto Barbosa Domont, and Fbio Csar Sousa Nogueira 24. 1H NMR Metabolomic Profiling of Human and Animal Blood Serum Samples Joo G.M. Pontes, Antonio J.M. Brasil, Guilherme C.F. Cruz, Rafael N. de Souza, and Ljubica Tasic 25. Lab-on-a-Chip Multiplex Assays Harald Peter, Julia Wienke, and Frank F. Bier 26. Multiplex Smartphone Diagnostics Juan L. Martinez-Hurtado, Ali K. Yetisen, and Seok H. Yun 27. Development of a User-Friendly App for Assisting Anticoagulation Treatment Johannes Vegt Part IV: Future Directions 28. Multiplex Biomarker Approaches to Enable Point-of-Care Testing and Personalized Medicine Paul C. Guest
