Description
The modern fascination with micro- and nano-sized materials can actually be traced back further to the 1960s and ’70s when the first few reported attempts were made to use nanoparticles for controlled drug delivery. In Nanoparticles in Biology and Medicine: Methods and Protocols, experts in the field present a wide range of methods for synthesis, surface modification, characterization, and application of nano-sized materials (nanoparticles) in life science and medical fields, mostly for drug delivery. The methods presented cover all stages of nanoparticle manufacturing, modification, analysis, and applications. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Nanoparticles in Biology and Medicine: Methods and Protocols will help the beginner become familiar with this fascinating field and will provide scientists at all levels of expertise with easy-to-follow practical advice needed to make, modify, and analyze nanoparticles of their choice and to use them in a wide range of biomedical and pharmaceutical applications, including functional protein studies, drug delivery, immunochemistry, imaging, and many others. Part I: Noble Metal Nanoparticles: Synthesis and Applications 1. Procedures for the Synthesis and Capping of Metal Nanoparticles Claudia Gutirrez-Wing, J. Jess Velzquez-Salazar, and Miguel Jos-Yacamn 2. Preparation of Concentrated Colloids of Gold Core-Silica Shell Nanoparticles for Biomedical Applications Yeon-Su Park 3. Biosynthesis of Silver and Gold Nanoparticles Using Bacillus licheniformis Muthu Irulappan Sriram, Kalimuthu Kalishwaralal, and Sangiliyandi Gurunathan 4. Gold Nanoparticle Antibody Conjugates for Use in Competitive Lateral Flow Assays Julian Bailes, Samantha Mayoss, Phil Teale, and Mikhail Soloviev 5. Gold Nanoparticles for Immunogold Localization of Antigens in Plant Tissues Sadaki Yokota 6. RNA Quantification Using Noble Metal Nanoprobes: Simultaneous Identification of Several Different mRNA Targets Using Colour Multiplexing and Application to Cancer Diagnostics Joao Conde, Goncalo Doria, Jesus M. de la Fuente, and Pedro Viana Baptista 7. Effect of Gold Nanoparticle Conjugation on the Activity and Stability of Functional Proteins Julian Bailes, Sara Gazi, Rositsa Ivanova, and Mikhail Soloviev Part II: Semiconductor Nanoparticles: Synthesis and Applications 8. Synthesis of Semiconductor Nanoparticles Xianfeng Chen and Peter J. Dobson 9. Size-Selective Synthesis of Ultrasmall Hydrophilic CdSe Nanoparticles in Aqueous Solution at Room Temperature Yeon-Su Park, Yukihiro Okamoto, Noritada Kaji, Manabu Tokeshi, and Yoshinobu Baba 10. Synthesis and Functionalization of CdSe/ZnS QDs Using the Successive Ion Layer Adsorption Reaction and Mercaptopropionic Acid Phase Transfer Methods Ana Sofia Miguel, Christopher Maycock, and Abel Oliva 11. In Vivo, Site-Specific, Covalent Conjugation of Quantum Dots to Proteins Via Split-Intein Splicing Anna Charalambous, Maria Andreou, Ioanna Antoniades, Neophytos Christodoulou, and Paris A. Skourides 12. Evaluating the Potential of Quantum Dots for In Vitro Biological Studies: Effects on Gene Expression Using Microarray Analysis Babu R. Prasad, Enda O’ Connell, Terry J. Smith, Valrie A. Grard, Yurii K. Gun’ko, and Yury Rochev 13. The Use of Quantum Dots for Immunochemistry Applications Helena Montn, Mnica Roldn, Arben Merkoi, Emma Rossinyol, Onofre Castell, and Carme Nogus 14. Quantum Dots for Labelling Live Cells Yun Chen 15. Labeling and Imaging Mesenchymal Stem Cells with Quantum Dots Maria C. Collins, Philip R. Gunst, Wayne E. Cascio, Alan P. Kypson, and Barbara J. Muller-Borer 16. The Application of Semiconductor Quantum Dots for Enhancing Peptide Desorption, Improving Peak Resolution and Sensitivity of Detection in Matrix-Assisted Laser Desorption/Ionization (MALDI) Mass Spectrometry Julian Bailes and Mikhail Soloviev Part III: Magnetic Nanoparticles: Synthesis and Applications 17. Near-Infrared Fluorescence Labeling of Iron Nanoparticles and Applications for Cell Labeling and In Vivo Imaging Jinke Wang, Yingxun Liu, Yong Hou, Zhongpin Chen, and Ning Gu 18. Labeling Stem Cells with Superparamagnetic Iron Oxide Nanoparticles: Analysis of the Labeling Efficacy by Microscopy and Magnetic Resonance Imaging Jasmin, Ana Luiza Machado Torres, Linda Jelicks, Antonio Carlos Campos de Carvalho, David C. Spray, and Rosalia Mendez-Otero 19. Non-Invasive Quantification of Magnetic Nanoparticles by Means of Magnetorelaxometry Maik Liebl and Dietmar Eberbeck 20. Synthesis, Application, and Tracking of Magnetic Carbon-Coated Nanoparticles in Plants Alejandro Prez-de-Luque, Zuny Cifuentes, Clara Marquina, Jess M. de la Fuente, and M. Ricardo Ibarra Part IV: Organic, Polymeric, Biomimetic, and Biocompatible Nanoparticles and Their Applications 21. Synthesis of Biocompatible Gelatinated Thioglycolic Acid-Capped CdTe Quantum Dots (“Jelly Dots”) Valrie A. Grard, Yurii K. Gun’ko, Babu R. Prasad, and Yury Rochev 22. Preparation and Characterization of Biomimetic Nanoparticles for Drug Delivery Ana Maria Carmona-Ribeiro 23. Preparation of Nanoparticles by Spray-Drying and Their Use for Efficient Pulmonary Drug Delivery Hao-Ying Li and Fan Zhang 24. Synthesis and Evaluation of Airway Targeted PLGA Nanoparticles for Drug Delivery in Obstructive Lung Diseases Neeraj Vij 25. Preparation of siRNA-Encapsulated PLGA Nanoparticles for Sustained Release of siRNA and Evaluation of Encapsulatiuon Efficiency Panayotis Pantazis, Konstantinos Dimas, James H. Wyche, Shrikant Anant, Courtney W. Houchen, Jayanth Panyam, and Rama P. Ramanujam 26. Synthesis of Poly (2-hydroxyethyl methacrylate) (PHEMA) Based Nanoparticles for Biomedical and Pharmaceutical Applications Rajesh Saini, Jaya Bajpai, and Anil K. Bajpai 27. Preparation of Lipid:Peptide:DNA (LPD) Nanoparticles and Their Use for Gene Transfection Fan Zhang and Hao-Ying Li 28. Crotamine: A Novel Cell-Penetrating Polypeptide Nanocarrier with Potential Anti-Cancer and Biotechnological Applications Mirian A.F. Hayashi, Eduardo B. Oliveira, Irina Kerkis, and Richard L. Karpel 29. Elastin-Based Nanoparticles for Delivery of Bone Morphogenetic Proteins Raul Machado, Paulo C. Bessa, Rui L. Reis, Jos Carlos Rodriguez-Cabello, and Margarida Casal 30. Synthesis, Surface Modification, Characterization, and Biomedical In Vitro Applications of Organically Modified Silica (ORMOSIL) Nanoparticles Diksha and Indrajit Roy 31. Extraction of Organic Nanoparticles from Plants Scott C. Lenaghan, Qin Zhu, Lijin Xia, and Mingjun Zhang Part V: Nano-Toxicity and Nano-Safety 32. Assessment of Cytotoxicity of Carbon Nanoparticles Using 3-(4,5-Dimethylthiazol-2-yl)-5-(3-Carboxymethoxyphenyl)-2-(4-Sulfophenyl)-2H-Tetrazolium (MTS) Cell Viability Assay Amanda M. Schrand, Jonathan B. Lin, and Saber M. Hussain 33. Luciferase Reporter System for Studying the Effect of Nanoparticles on Gene Expression Min Ding, Linda Bowman, and Vincent Castranova 34. Single Cell Gel Electrophoresis Assay (Comet Assay) for Evaluating Nanoparticles-Induced DNA Damage in Cells Linda Bowman, Vincent Castranova, and Min Ding 35. Assessment of Toxicity of Nanoparticles Using Insects as Biological Models Yan Zhou, Aracely Rocha, Carlos J. Sanchez, and Hong Liang 36. Evaluation of Cytotoxicity of 3-Mercaptopropionic Acid-Modified Quantum Dots on Medicago sativa Cells and Tissues Ana Raquel Santos, Ana Sofia Miguel, Pedro Fevereiro, and Abel Oliva Part VI: Nanoparticles Characterization 37. Scanning Transmission Electron Microscopy Methods for the Analysis of Nanoparticles Arturo Ponce, Sergio Meja-Rosales, and Miguel Jos-Yacamn 38. Total Internal Reflection Microscopy (TIRF) for Real-Time Imaging of Nanoparticle-Cell Plasma Membrane Interaction Ladan Parhamifar and S. Moein Moghimi 39. Microscopy Imaging Methods for the Detection of Silver and Titanium Nanoparticles Within Cells Robert Martin Zucker and Kaitlin Marie Daniel 40. Detection of TiO2 Nanoparticles in Cells by Flow Cytometry Robert Martin Zucker and Kaitlin Marie Daniel 41. NanoParticle Tracking Analysis for the Multiparameter Characterization and Counting of Nanoparticle Suspensions Matthew Wright 42. Relevance of Two-Dimensional Brownian Motion Dynamics in Applying Nanoparticle Tracking Analysis Paul Van der Meeren, Marios Kasinos, and Hans Saveyn 43. Nanoparticle Bridges for Studying Electrical Properties of Organic Molecules Klaus Leifer, Ken Welch, Syed Hassan Mujtaba Jafri, and Tobias Blom
