Description
The discovery of stem and progenitor cells in the adult mammalian CNS challenged the long standing “no new neuron” doctrine and opened the door to the potential for cell replacement therapy. The process from discovery to clinical applications can be long and tortuous, requiring rigorous steps involving standardized and precise protocols. Neural Progenitor Cells: Methods and Protocols is a collection of practical articles describing techniques used to study neural stem and progenitor cells. The volume also highlights the promise of stem cell-based therapeutic applications for CNS disorders. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Neural Progenitor Cells: Methods and Protocols serves both professionals and novices with its well-honed methodologies. Part I- Culturing Neural Precursor Cells from Primary Tissue 1. Culturing Fetal Precursors Cells Using Free-Floating Serum-Free Conditions Andrew Chojnacki and Samuel Weiss 2. In vitro Expansion of Foetal Neural Progenitors as Adherent Cell Lines Steven M. Pollard 3. Isolate and Culture Precursor Cells from the Adult Periventricular Area Chiara Cavazzin, Margherita Neri, and Angela Gritti 4. Culturing and Expansion of Precursor Cells from the Adult Hippocampus Dhanisha J. Jhaveri, Boris W. Prosper, and Perry F. Bartlett 5. Isolate and Culture Neural Stem Cells from the Mouse Adult Spinal Cord Jean-Philippe Hugnot 6. Culturing and Expansion of “Clinical Grade” Precursors Cells from the Foetal Human Central Nervous System Maurizio Gelati, Daniela Profico, Massimo Projetti-Pensi, Gianmarco Muzi, Giada Sgaravizzi, and Angelo Luigi Vescovie 7. Isolating and Culturing of Precursor Cells from the Adult Human Brain Florian A. Siebzehnrubl and Dennis A. Steindler 8. Isolation and Culture of Precursor Cells from the Adult Human Spinal Cord Luc Bauchet, Nicolas Lonjon, Florence Vachiery-Lahave, Alain Boularan, Alain Privat, and Jean-Philippe Hugnot 9. Isolation and Enrichment of Defined Neural Cell Populations from Heterogeneous Neural Stem Cell Progeny Hassan Azari 10. Isolation of Adult Stem Cells from the Human Olfactory Mucosa Franois Fron, Chris Perry, Stphane D. Girard, and Alan Mackay-Sim Part II. Stem Cells vs Progenitor Cells 11. Enumerating Stem Cell Frequency – Neural Colony Forming Cell Assay Sharon A. Louis and Carmen K. H. Mak Part III. Purification of Stem and Progenitor Cells 12. Flow Cytometry of Neural Cells Geoffrey W. Osborne Part IV. Transplantation of Neural Progenitor Cells: Preclinical models 13. Neonatal Transplant in Hypoxic Injury Tong Zheng and Michael D. Weiss 14. Isolation and Purification of Self Renewable Human Neural Stem Cells for Cell Therapy in Experimental Model of Ischemic Stroke Ricardo L. Azevedo-Pereira and Marcel M. Daadi 15. Transplantation of Foetal Midbrain Dopamine Progenitors into a Rodent Model of Parkinson’s Disease Lachlan H. Thompson and Clare L. Parish Part V. In vivo Identification of Neural Precursor Cells 16. Distribution of Neural Precursor Cells in Rodent Brain Daniel G. Blackmore and Rodney L. Rietze 17. Identifying Neural Precursor Cells in the Adult Human Brain Thomas I.H. Park, Henry J. Waldvogel, Johanna M. Montgomery, Edward W. Mee, Peter S. Bergin, Richard L.M. Faull, Mike Dragunow, and Maurice A. Curtis Part VI. Clinical Application of Neural Precursor Cell Therapy 18. Clinical Trials for the Treatment of Spinal Cord Injury: Not So Simple Alan Mackay-Sim and Franois Fron
