Description
The volume aims at providing an overview of cutting-edge methods and protocols used in neuroscience and neurological research. Neurotransmitter Transporters: Investigative Methods will be of interest to scientists, graduate students, and advanced undergraduates who seek an overview of methods and protocols in this field of neuroscience. It will also be of interest to physicians who are carrying out imaging and postmortem studies of neurotransmitter transporters in the human brain. Written for the popular Neuromethods series, chapters include the kind of detail and key implementation advice that ensures successful results in the laboratory. Authoritative and practical, Neurotransmitter Transporters: Investigative Methods aims to ensure successful results in the further study of this vital field. Part I: Recent Improvements in LV Construction, Production, and Transduction 1. Construction of Modular Lentiviral Vectors for Effective Gene Expression and Knockdown Angeline De Bruyns, Ben Geilin, and David Dankort 2. Development of Inducible Molecular Switches Based on All-In-One Lentiviral Vectors Equipped With Drug Controlled FLP Recombinase Tobias Maetzig and Axel Schambach 3. Production Of Retrovirus-Based Vectors In Mildly Acidic Ph Conditions Nathalie Holic And David Fenard 4. Optimized Lentiviral Transduction Protocols by use of a Poloxamer Enhancer, Spinoculation and Scfv-Antibody Fusions To VSV-G NataSa Anastasov, Ines Hfig, Sabine Mall, Angela M. Krackhardt, and Christian Thirion Part II: New LV Targets and Applications 5. Transduction of Murine Hematopoietic Stem Cells with Tetracycline-Regulated Lentiviral Vectors Maike Stahlhut, Axel Schambach, and Olga S. Kustikova 6. Introduction of Shrnas, Mirnas or Antagomirs into Primary Human Liver Cells Through Lentiviral Vectors Jessica K. Rieger and Maria Thomas 7. Production and Concentration of Lentivirus for Transduction of Primary Human T Cells Alan Kennedy and Adam P. Cribbs 8. Generating Transgenic Mice by Lentiviral Transduction of Spermatozoa Followed by In Vitro Fertilization and Embryo Transfer Anil Chandrashekran, Colin Casimir, Nick Dibb, Carol Readhead, and Robert Winston 9. The LAM-PCR Method to Sequence LV Integration Sites Wei Wang, Cynthia C. Bartholomae, Richard Gabriel, Annette Deichmann, and Manfred Schmidt 10. Conditional Rnai using the Lentiviral GLTR System Elisabeth Pfeiffenberger and Stephan Geley 11. Lentiviral Vectors for the Engineering of Implantable Cells Secreting Recombinant Antibodies Aurlien Lathuilire and Bernard L. Schneider III. Integrase-Mutant Lvs 12. Transient Expression of Green Fluorescent Protein in Integrase-Defective Lentiviral Vector Transduced 293T Cell Line Fazlina Nordin, Zariyantey Abdul Hamid, Lucas Chan, Farzin Farzaneh, and MK Azaham A Hamid 13. Intrastriatal Delivery of Integration-Deficient Lentiviral Vectors in a Rat Model of Parkinson’s Disease Ngoc B. Lu-Nguyen, Martin Broadstock, and Rafael J. Yez-Muoz 14. Development of Lentiviral Vectors for Targeted Integration and Protein Delivery Diana Schenkwein and Seppo Yl-Herttuala Part IV: Production, Detection, and Engineering of Exosomes 15. Biogenesis and Functions of Exosomes and Extracellular Vesicles Florian Dreyer and Andreas Baur 16. Generation, Quantification, and Tracing of Metabolically Labeled Fluorescent Exosomes Carolina Coscia, Isabella Parolini, Massimo Sanchez, Mauro Biffoni, Zaira Boussadia, Cristiana Zanetti, Maria Luisa Fiani, and Massimo Sargiacomo 17. Cardiac Myocyte Exosome Isolation Zulfiqar A. Malik and Anne A. Knowlton 18. Incorporation of Heterologous Proteins in Engineered Exosomes Francesco Manfredi, Paola Di Bonito, Claudia Arenaccio, Simona Anticoli, and Maurizio Federico 19. Exosome-Mediated Targeted Delivery of Mirnas Shin-Ichiro Ohno and Masahiko Kuroda
