Description
This volume collects protocols and procedures utilizing cellular, tissue, and whole animal models that can be applied to the investigation of neurotrophic factors and other agents impacting on these systems. It begins with chapters on the culture of neurons and glia from the central and peripheral nervous systems, neuron-glia and glia-glia co-culture models, oligodendrocytes, and cell-based assays for the evaluation of cell vitality. This revised second edition expands to cover methodology encompassing site-specific direct labeling of neurotrophins and their receptors, angiogenesis assays, stem cells, pancreatic beta-cells, axonal transport, synapse biology, dendritic spine analysis, and brain endothelial cells. Subsequent chapters are dedicated to in vivo lesion models of relevance to nervous system pathology, which can be applied to the investigation of neurotrophic factors and peptides, as well as protocols describing nanofiber- and nanoparticle-based methods for brain delivery of neurotrophic agents. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Neurotrophic Factors: Methods and Protocols, Second Edition is a valuable resource for a wide audience of readers as they explore nervous system function and pathology. 1. Neurotrophic Factors: An Overview Stephen D. Skaper 2. Ocular Nerve Growth Factor (NGF) and NGF Eye Drop Application as Paradigms to Investigate NGF Neuroprotective and Reparative Actions Paola Tirassa, Pamela Rosso, and Angela Iannitelli 3. Culture of Rodent Cortical, Hippocampal, and Striatal Neurons Laura Facci and Stephen D. Skaper 4. Culture of Neonatal Rodent Microglia, Astrocytes, and Oligodendrocytes from Cortex, Spinal Cord, and Cerebellum Stephen D. Skaper and Laura Facci 5. Central Nervous System Neuron-Glia Co-Culture Models and Application to Neuroprotective Agents Stephen D. Skaper and Laura Facci 6. Preparation of Adult Rat Sensory Neuron Cultures and Their Application to Growth Cone Turning Assays Christine A. Webber and Douglas W. Zochodne 7. Sensory Neurons from Tau Transgenic Mice and Their Utility in Drug Screening Aviva M. Tolkovsky and Jack Brelstaff 8. Culture of Rat Mesencephalic Dopaminergic Neurons and Application to Neurotoxic and Neuroprotective Agents Stephen D. Skaper, Massimo Barbierato, Vanni Ferrari, Morena Zusso, and Laura Facci 9. Culture of Neurons and Smooth Muscle Cells from the Myenteric Plexus of Adult Mice Paola Brun and Hamid I. Akbarali 10. Astrocyte/Microglia Co-Cultures as a Model to Study Neuroinflammation Laura Facci, Massimo Barbierato, and Stephen D. Skaper 11. Micropatterned Geometries Shape Oligodendrocyte and Microglia Plasticity Cinzia Volont, Adele De Ninno, and Susanna Amadio 12. Oligodendrocyte Progenitor Cell Cultures: A Model to Screen Neurotrophic Compounds for Myelin Repair Massimo Barbierato, Stephen D. Skaper, and Laura Facci 13. Cell Enumeration Assays: Application of the MTT and Sulforhodamine B Assays to Lipopolysaccharide-Stimulated Neonatal Rodent Microglia Laura Facci and Stephen D. Skaper 14. Diolistic Labeling and Analysis of Dendritic Spines M. Foster Olive, Armani P. Del Franco, and Cassandra D. Gipson 15. Culture of Mouse Giant Central Nervous System Synapses and Application for Imaging and Electrophysiological Analyses Dimitar Dimitrov, Laurent Guillaud, Kohgaku Eguchi, and Tomoyuki Takahashi 16. Methods for Quantitative Analysis of Axonal Cargo Transport Matias Alloatti, Luciana Bruno, and Tomas L. Falzone 17. Neuronal Cell Morphology in Primary Cerebellar Granule Cells Using High-Content Analysis Nicholas Radio and Steven Frank 18. Nerve Growth Factor-Induced Angiogenesis: 1. Endothelial Cell Tube Formation Assay Philip Lazarovici, Adi Lahiani, Galit Gincberg, Dikla Hacham, Arnon Fluksman, Ofra Benny, Cezary Marcinkiewicz, and Peter I. Lelkes 19. Nerve Growth Factor-Induced Angiogenesis: 2. The Quail Chorioallantoic Membrane Assay Philip Lazarovici, Adi Lahiani, Galit Gincberg, Dikla Haham, Cezary Marcinkiewicz, and Peter I. Lelkes 20. Rat Pancreatic Beta-Cell Culture Myrian Velasco, Carlos Larqu, Carlos Manlio Daz-Garca, Carmen Sanchez-Soto, and Marcia Hiriart 21. Growth and Neurotrophic Factors in Embryonic Stem Cells Micaela Pannella, Luciana Giardino, Laura Calz, and Mercedes Fernndez 22. Site-Specific Direct Labeling of Neurotrophins and Their Receptors: From Biochemistry to Advanced Imaging Applications Francesco Gobbo, Fulvio Bonsignore, Rosy Amodeo, Antonino Cattaneo, and Laura Marchetti 23. Isolation and Culture of Brain Microvascular Endothelial Cells for In Vitro Blood-Brain Barrier Studies Hector Rosas-Hernandez, Elvis Cuevas, Susan M. Lantz, Merle G. Paule, and Syed F. Ali 24. Preparation of Rat Hippocampal Organotypic Cultures and Application to Study Amyloid -Peptide Toxicity Maria Rosanna Bronzuoli, Roberta Facchinetti, and Caterina Scuderi 25. An Animal Model of Alzheimer Disease Based on the Intrahippocampal Injection of Amyloid -Peptide (1-42) Roberta Facchinetti, Maria Rosanna Bronzuoli, and Caterina Scuderi 26. Active Induction of Experimental Autoimmune Encephalomyelitis in C57BL/6 Mice Gabriella Contarini, Pietro Giusti, and Stephen D. Skaper 27. A Model of Systemic Inflammation to Study Neuroinflammation Vincenzo Sorrenti, Pietro Giusti, and Morena Zusso 28. Spared Nerve Injury as a Long-Lasting Model of Neuropathic Pain Serena Boccella, Francesca Guida, Enza Palazzo, Ida Marabese, Vito de Novellis, Sabatino Maione, and Livio Luongo 29. An In Vivo Compression Model of Spinal Cord Injury Irene Paterniti, Emanuela Esposito, and Salvatore Cuzzocrea 30. A Controlled Cortical Impact Preclinical Model of Traumatic Brain Injury Michela Campolo, Emanuela Esposito, and Salvatore Cuzzocrea 31. Middle Cerebral Artery Occlusion by an Intraluminal Suture Method Rosalia Crupi, Rosanna Di Paola, Emanuela Esposito, and Salvatore Cuzzocrea 32. Primary Motor Neuron Culture to Promote Cellular Viability and Myelination Jun-Kyo Francis Suh and Sujin Hyung 33. Culture of Human Primary Bone Cells and Phenotype Assessment Paola Brun 34. Recreating Intestinal Peristalsis in the Petri Dish Rosa Espinosa-Luna, Alma R. Barajas-Espinosa, Fernando Ochoa-Cortez, and Carlos Barajas-Lpez 35. Synthesis of Biocompatible Titanate Nanofibers for Effective Delivery of Neuroprotective Agents Asya Ozkizilcik, Roger Williams, Z. Ryan Tian, Dafin F. Muresanu, Aruna Sharma, and Hari S. Sharma 36. Preparation and Characterization of Biocompatible Chitosan Nanoparticles for Targeted Brain Delivery of Peptides Muge Yemisci, Secil Caban, Eduardo Fernandez-Megia, Yilmaz Capan, Patrick Couvreur, and Turgay Dalkara
