Description
This volume explores the latest techniques used by researchers to help them better understand the NMDAR structure-function relations, principles, and rules that govern how NMDARs operate in brain processing. The volume includes a detailed introductory chapter describing the field and is divided into three parts. Topics covered in the volume include: quantification of NMDAR subunit genes expression by qRT-PCR; detection of NMDARs antibodies in encephalitis; recombinant channels in host cells using a fast agonist application system; GluNs detection and functions in microglial cells; and NMDARs as voltage sensors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, NMDA Receptors: Methods and Protocols is a valuable resource that discusses the diversity of the currently-used methods, the importance of NMDARs and their complexity, and the progress that has already been made. This book will be of interest to scientists, clinicians, and industry professionals working in this field. Preface… Table of Contents… Contributing Authors… 1. NMDA Receptors in the Central Nervous System Kasper B. Hansen, Feng Yi, Riley Perszyk, Frank S. Menniti, and Stephen F. Traynelis Part I NMDAR Subunits Expression and Mutations 2. Quantification of NMDAR Subunit Genes Expression by qRT-PCR Emilie Pallesi-Pocachard 3. Genetic and Functional Analysis of GRIN2A in Tumor Samples Todd D. Prickett, Jared J. Gartner, and Yardena Samuels 4. Detection of NMDARs Antibodies in Encephalitis Matteo Gastaldi, Patrick Waters, and Angela Vincent Part II Studying Structure-Function Relations in NMDARs 5. MagnetofectionTM of NMDA Receptor Subunits GluN1 and GluN2A Expression Vectors in Non-Neuronal Host Cells Nadine Bruneau and Pierre Szepetowski 6. Transfection in Primary Cultured Neuronal Cells Katie F. M. Marwick and Giles E. Hardingham 7. Selective Cell-Surface Expression of Triheteromeric NMDA Receptors Feng Yi, Stephen F. Traynelis, and Kasper B. Hansen 8. Functional Analysis of Recombinant Channels in Host Cells using a Fast Agonist Application System Andrei Rozov and David Jappy 9. GluN2B Subunit Labeling with Fluorescent Probes and High-Resolution Live Imaging C. Perrio, O. Nicole, and A. Buisson 10. Design of Light-Sensitive NMDARs by Genetically Encoded Photo-Cross-Linkers Meilin Tian and Shixin Ye Part III Analysis of NMDAR Function/Activity 11. Gene Targeted Mice with Conditional Knock-In (-Out) of NMDAR Mutations Rolf Sprengel, Ahmed Eltokhi, and Frank N. Single 12. Electrophysiological Investigation of NMDA Current Properties in Brain Slices Alexandre Pons Bennaceur and Natalia Lozovaya 13. Analysis of Functional NMDA Receptors in Astrocytes Frank Kirchhoff 14. GluNs Detection and Functions in Microglial Cells Stephane Peineau, Vincent Degos, Catherine Verney, and Pierre Gressens 15. NMDA Receptor Activity in Circulating Red Blood Cells: Methods of Detection Asya Makhro, Lars Kaestner, and Anna Bogdanova 16. NMDA Receptors as Voltage Sensors Roustem Khazipov 17. Development of a Computational Approach/Model to Explore NMDA Receptors Functions A. Florence Keller, Jean-Marie C. Bouteiller, and Theodore W. Berger
