Description
This detailed volume explores olfactory receptors, a topic that goes beyond classical biochemical essays and involves methods to investigate the brain areas associated with the perception of odorants. Gathering the strands of many advanced techniques, the book organizes these key methods in order to achieve a unifying comprehension of the olfactory system. Written for the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and multidisciplinary, Olfactory Receptors: Methods and Protocols serves to benefit not only those who are beginning in the field of olfactory receptors, but also those who are already working in the field and that want to learn another advanced techniques. 1. CD36 Neuronal Identity in the Olfactory Epithelium Andr Machado Xavier and Isaias Glezer 2. Deorphanization of Olfactory Trace Amine-Associated Receptors Qian Li 3. G Protein-Coupled Receptor Kinase 3 (GRK3) in Olfaction Sayoko Ihara and Kazushige Touhara 4. Virus-Mediated Overexpression of Vomeronasal Receptors and Functional Assessment by Live-Cell Calcium Imaging Pablo Chamero and Frank Zufall 5. Calcium Imaging of Individual Olfactory Sensory Neurons from Intact Olfactory Turbinates Chunbo Zhang 6. Fluorescence-Activated Cell Sorting of Olfactory Sensory Neuron Subpopulations Artur G. Leme Silva, Mara H. Nagai, and Bettina Malnic 7. Numerical Models and In Vitro Assays to Study Odorant Receptors Caroline Bushdid, Claire A. de March, Hiroaki Matsunami, and Jrme Golebiowski 8. High-Throughput Odorant Receptor Deorphanization Via pS6-Immunoprecipitation and mRNA Profiling Xiaoyang Serene Hu and Hiroaki Matsunami 9. Patch-Clamp Recordings from Mouse Olfactory Sensory Neurons Anna Boccaccio 10. In Vivo Electrophysiological Recordings of Olfactory Receptor Neuron Units and Electro-Olfactograms in Anesthetized Rats Patricia Duchamp-Viret and Michel Chaput 11. Suction Pipette Technique: An Electrophysiological Tool to Study Olfactory Receptor-Dependent Signal Transduction Michele Dibattista and Johannes Reisert 12. Odor-Induced Electrical and Calcium Signals from Olfactory Sensory Neurons In Situ Xavier Grosmaitre and Minghong Ma 13. Long-term Plasticity at the Mitral and Tufted Cell to Granule Cell Synapse of the Olfactory Bulb Investigated with a Custom Multi-Electrode in Acute Brain Slice Preparations Michael Lukas, Knut Holthoff, and Veronica Egger 14. Multi-Site Recording of Local Field Potentials in Awake, Free-Moving Mice Xingfeng Mao, Tiantian Cao, and Anan Li 15. In Vivo Two-Photon Imaging of the Olfactory System in Insects Marco Paoli and Albrecht Haase 16. Approaches for Assessing Olfaction in Children with Autism Spectrum Disorder Hirokazu Kumazaki, Masako Okamoto, Syo Kanzaki, Ken-ichi Okada, Masaru Mimura, Yoshio Minabe, and Mitsuru Kikuchi 17. Methods in Rodent Chemosensory Cognition Alan Gelperin 18. Bioelectronic Nose Using Olfactory Receptor-Embedded Nanodiscs Heehong Yang, Minju Lee, Daesan Kim, Seunghun Hong, and Tai Hyun Park 19. Tracking Odorant Plumes Matthew A. Reidenbach 20. Generative Biophysical Modeling of Dynamical Networks in the Olfactory System Guoshi Li and Thomas A. Cleland 21. Behavioral Assays in the Study of Olfaction: A Practical Guide Fabio Papes, Thiago S. Nakahara, and Antonio P. Camargo
