Description
This volume provides researchers with up-to-date protocols and takes a close look at current research and promising applications. Optogenetics: Methods and Protocols contains a collection of recently developed technical protocols on optogenetic applications in neuroscience, brain mapping, treatment of neurological disorders, and restoration of visual function. Several introductory and discussion chapters offer a wide overview about sources and diversity of optogenetic tools, design strategies, and potential applications in other fields like plant research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting edge and thorough, Optogenetic: Methods and Protocols, delivers an interdisciplinary view of optogenetic applications and its potential to develop as a valuable tool for basic research, as well as biomedical and biotechnological applications. 1. Optogenetics: Basic Concepts and Their DevelopmentYong Ku Cho and Dan Li2. Natural Resources for Optogenetic ToolsTilo Mathes3. Algal Photobiology: A Rich Source of Unusual Light Sensitive Proteins for Synthetic Biology and OptogeneticsArash Kianianmomeni and Armin Hallmann4. Reversible Photoregulation of gene Expression and TranslationShinzi Ogasawara5. Controlling Protein Activity and Degradation Using Blue-LightAnne P. Lutz, Christian Renicke, and Christof Taxis6. Photo-Control of Protein Function Using Photoactive Yellow ProteinJakeb M. Reis and G. Andrews Woolley7. A Fluorometric Activity Assay for Light-Regulated Cyclic-Nucleotide-Monophosphate ActuatorsCharlotte Helene Chumacher, Heinz G. Krschen, Christopher Nicol, Carlos Gasser, Reinhard Seifert, Martin Schwrzel, and Andreas Mglich8. Optogenetic Control of Pancreatic IsletsThomas M. Reinbothe and Ins G. Mollet9. Optogenetics in Plants: Red/Far-Red Light Control of Gene ExpressionRocio Ochoa-Fernandez, Sophia L. Samodelov, Simon M. Brandl, Elke Wehinger, Konrad Mller, Wilfried Weber, and Matias D. Zurbriggen10. Perfect Channelrhodopsins: A User GuideJonas Wietek and Matthias Prigge11. Optogenetics in Drosophila NeuroscienceThomas Riemensperger, Robert J. Kittel, and Andr Fiala12. Optogenetic Control of Mammalian Ion Channels with Chemical PhotoswitchesDamien Lemoine, Romain Durand-de Cuttoli, and Alexandre Mourot13. Optogenetic Modulation of Locomotor Activity on Free Behaving RatsKedi Xu, Jiacheng Zhang, Songchao Guo, and Xiaoxiang Zheng14. Combines Optogenetic and Chemogenetic Control of NeuronsKen Berglund, Jack K. Tung, Bryan Higashikubo, Robert E. Gross, Christopher I. Moore, and Ute Hochgeschwender15. Intracranial Injection of an Optogenetics Viral Vector Followed by Optical Cannula Implantation for Neural Stimulation in Rat Brain CortexChristopher Pawela, Edgar DeYoe, and Ramin Pashaie16. An Optomized Calcium-Phosphate Transfection Method for Characterizing Genetically Encoded Tools in Primary NeuronsShiyao Wang and Yong Ku Cho17. Optogenetic Approaches for Mesoscopic Brain MappingMichael Kyweriga and Majid H. Mohajerani18. Optogenetic Tools for Confined Stimulation in Deep Brain StructuresAlexandre Castonguay, Sbastien Thomas, Frdric Lesage, and Christian Casanova19. Remote Patterning of Transgene Expression Using Near Infrared-Responsive Plasmonic HydrogelsFrancisco Martn-Saavedra and Nuria Vilaboa20. Optogenetic Light Crafting Tools for the Control of Cardiac ArrhythmiasClaudia Richter, Jan Christoph, Stephan E. Lehnart, and Stefan Luther21. Inscribing Optocal Excitability to Non-Excitable Cardiac Cells: Viral Delivery of Optogenetic Tools in Primary Cardiac FibroblastsJinzhu Yu and Emilia Entcheva22. Optogenetic Engineering of Atrial CardiomyocytesIolanda Feola, Alexander Teplenin, Antoine A.F. de Vries, and Danil A. Pijnappels23. A Multi-Channel Recording System with Optical Stimulation for Closed-Loop Optogenetic ExperimentsCarmen Bartic, Francesco P. Battaglia, Ling Wang, Thoa T. Nguyen, Henrique Cabral, and Zaneta Navratilova24. Optogenetic Control of Fibroblast Growth Factor Receptor SignalingNury Kim, Jin Man Kim, and Won Do Heo25. Protein Inactivation by Optogenetic Trapping in Living CellsHyerim Park, Sangkyu Lee, and Won Do Heo26. Optogenetic Manipulation of Selective Neural Activity in Free-Moving Drosophila AdultsPo-Yen Hsiao, Ming-Chin Wu, Yen-Yin Lin, Chein-Chung Fu, and Ann-Shyn Chiang27. Guidelines for Photoreceptor EngineeringThea Ziegler, Charlotte Helene Schumacher, and Andreas Mglich
