Description
This volume provides researchers with up-to-date protocols and takes a close look at current research and promising applications. Optogenetics: Methods and Protocols contains a collection of recently developed technical protocols on optogenetic applications in neuroscience, brain mapping, treatment of neurological disorders, and restoration of visual function. Several introductory and discussion chapters offer a wide overview about sources and diversity of optogenetic tools, design strategies, and potential applications in other fields like plant research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting edge and thorough, Optogenetic: Methods and Protocols, delivers an interdisciplinary view of optogenetic applications and its potential to develop as a valuable tool for basic research, as well as biomedical and biotechnological applications. 1. Optogenetics: Basic Concepts and Their Development Yong Ku Cho and Dan Li 2. Natural Resources for Optogenetic Tools Tilo Mathes 3. Algal Photobiology: A Rich Source of Unusual Light Sensitive Proteins for Synthetic Biology and Optogenetics Arash Kianianmomeni and Armin Hallmann 4. Reversible Photoregulation of gene Expression and Translation Shinzi Ogasawara 5. Controlling Protein Activity and Degradation Using Blue-Light Anne P. Lutz, Christian Renicke, and Christof Taxis 6. Photo-Control of Protein Function Using Photoactive Yellow Protein Jakeb M. Reis and G. Andrews Woolley 7. A Fluorometric Activity Assay for Light-Regulated Cyclic-Nucleotide-Monophosphate Actuators Charlotte Helene Chumacher, Heinz G. Krschen, Christopher Nicol, Carlos Gasser, Reinhard Seifert, Martin Schwrzel, and Andreas Mglich 8. Optogenetic Control of Pancreatic Islets Thomas M. Reinbothe and Ins G. Mollet 9. Optogenetics in Plants: Red/Far-Red Light Control of Gene Expression Rocio Ochoa-Fernandez, Sophia L. Samodelov, Simon M. Brandl, Elke Wehinger, Konrad Mller, Wilfried Weber, and Matias D. Zurbriggen 10. Perfect Channelrhodopsins: A User Guide Jonas Wietek and Matthias Prigge 11. Optogenetics in Drosophila Neuroscience Thomas Riemensperger, Robert J. Kittel, and Andr Fiala 12. Optogenetic Control of Mammalian Ion Channels with Chemical Photoswitches Damien Lemoine, Romain Durand-de Cuttoli, and Alexandre Mourot 13. Optogenetic Modulation of Locomotor Activity on Free Behaving Rats Kedi Xu, Jiacheng Zhang, Songchao Guo, and Xiaoxiang Zheng 14. Combines Optogenetic and Chemogenetic Control of Neurons Ken Berglund, Jack K. Tung, Bryan Higashikubo, Robert E. Gross, Christopher I. Moore, and Ute Hochgeschwender 15. Intracranial Injection of an Optogenetics Viral Vector Followed by Optical Cannula Implantation for Neural Stimulation in Rat Brain Cortex Christopher Pawela, Edgar DeYoe, and Ramin Pashaie 16. An Optomized Calcium-Phosphate Transfection Method for Characterizing Genetically Encoded Tools in Primary Neurons Shiyao Wang and Yong Ku Cho 17. Optogenetic Approaches for Mesoscopic Brain Mapping Michael Kyweriga and Majid H. Mohajerani 18. Optogenetic Tools for Confined Stimulation in Deep Brain Structures Alexandre Castonguay, Sbastien Thomas, Frdric Lesage, and Christian Casanova 19. Remote Patterning of Transgene Expression Using Near Infrared-Responsive Plasmonic Hydrogels Francisco Martn-Saavedra and Nuria Vilaboa 20. Optogenetic Light Crafting Tools for the Control of Cardiac Arrhythmias Claudia Richter, Jan Christoph, Stephan E. Lehnart, and Stefan Luther 21. Inscribing Optocal Excitability to Non-Excitable Cardiac Cells: Viral Delivery of Optogenetic Tools in Primary Cardiac Fibroblasts Jinzhu Yu and Emilia Entcheva 22. Optogenetic Engineering of Atrial Cardiomyocytes Iolanda Feola, Alexander Teplenin, Antoine A.F. de Vries, and Danil A. Pijnappels 23. A Multi-Channel Recording System with Optical Stimulation for Closed-Loop Optogenetic Experiments Carmen Bartic, Francesco P. Battaglia, Ling Wang, Thoa T. Nguyen, Henrique Cabral, and Zaneta Navratilova 24. Optogenetic Control of Fibroblast Growth Factor Receptor Signaling Nury Kim, Jin Man Kim, and Won Do Heo 25. Protein Inactivation by Optogenetic Trapping in Living Cells Hyerim Park, Sangkyu Lee, and Won Do Heo 26. Optogenetic Manipulation of Selective Neural Activity in Free-Moving Drosophila Adults Po-Yen Hsiao, Ming-Chin Wu, Yen-Yin Lin, Chein-Chung Fu, and Ann-Shyn Chiang 27. Guidelines for Photoreceptor Engineering Thea Ziegler, Charlotte Helene Schumacher, and Andreas Mglich
