Description
The quantitative analysis of biospecific interactions is an area of increasing importance in the course of going from qualitative description of biological systems to advanced quantitative analysis of molecular events in biological processes. A thorough understanding of the kinetics, equilibria and dynamics of noncovalent interactions in living material is an essential step forward to connect basic biochemistry with physiology and pharmacology. This volume covers quantitative biospecific analysis as its sole topic. It provides clear descriptions of state-of-the-art procedures for analysis of equilibria and kinetics of biospecific interactions, with straightforward example of applications to biochemical problems. This book is described in both immobilized and free systems. The analysis of affinities for membrane proteins in lipid bilayers discovered, as well as analyses of interactions with soluble proteins. authors discuss integrated optics techniques, surface plasmon resonance detection, electrophoretic and chromatographic methods and, briefly, binding studies by centrifugation and filtration techniques.
