Description
Activation, inhibition, or destruction of the nervous system or its component parts as a vital tool for the investigation of function has undergone remarkable development; indeed, new approaches have been developed that allow for these actions to be used as therapeutic tools. In Stimulation and Inhibition of Neurons, experts in the field provide an overview of modern methods for generating lesions as well as for stimulating and inhibiting neural pathways. Many new techniques such as optogenetics and the use of the in situ perfused preparation are examined, while, in other sections, the use and validity of more well-known approaches are reassessed. Written for the Neuromethods series, chapters examine their respective topics thoroughly and include the kind of detail and implementation advice that ensures successful results in the laboratory. Authoritative and cutting-edge, Stimulation and Inhibition of Neurons serves as an ideal guide for researchers seeking to gain further knowledge of the complex functions of the brain. Part I: Novel Experimental Techniques 1. Optogenetic Stimulation of Neurons in the Brainstem of Freely Behaving Rats and Mice Stephen Abbott 2. Stimulating Peripheral Afferents to Evoke Cardiorespiratory Reflex responses in the In Situ Arterially-Perfused Preparation Angelina Y. Fong 3. Excitatory Responses to Microinjection of Glutamate Depend on Dose not Volume: A Meta-Analysis of Studies in Rat RVLM Andrea H. Gaede and Paul M. Pilowsky 4. Acute Activation and Inhibition of the Sympathetic Baroreceptor Reflex Melissa M.J. Farnham Part II: Intracellular Signalling 5. Pharmacological Dissection of G Protein-Mediated Second Messenger Cascades in Neurons Mark C. Bellingham 6. In Vivo Manipulation of Intracellular Signalling Pathways V.J. Tallapragada Part III: The Single Neurons 7. Identification of Spinally Projecting Neurons in the Rostral Ventrolateral Medulla In Vivo Simon McMullan 8. Microionotophoretic Study of Individual Neurons During Intracellular Recording Qi-Jian Sun and Paul M. Pilowsky 9. Neurobiotin Electroporation for Combined Structural and Functional Analysis of Neurons in Developing Mouse Brain Slices Refik Kanjhan and Mark C. Bellingham 10. Juxtacellular Neuronal Labelling, Physiological Characterization, and Phenotypic Identification of Single Neurons In Vivo Anthony J.M. Verberne and Ida J. Llewellyn-Smith Part IV: Methods for Analysis 11. Analysis of Sympathetic Nerve Activity Analysis of Sympathetic Nerve Activity Rohit Ramchandra 12. Insight into Autonomic Nervous System Control of Heart Rate in the Rat Using Analysis of Heart Rate Variability and Baroreflex Sensitivity Cara M. Hildreth, Ann K. Goodchild, and Jacqueline K. Phillips 13. Neurophysiological Recording of the Compound Muscle Action Potential for Motor Unit Number Estimation in Mice Shyuan T. Ngo and Mark C. Bellingham Part V: Clinical Focus and Application 14. Animal Models of Neuropathic Pain Due to Nerve Injury Paul J. Austin and Gila Moalem-Taylor 15. Detection of Sensitized Nerve Responses: Dorsal Root Reflexes, Live Cell Calcium, and ROS Imaging Karin N. Westlund and Liping Zhang 16. Visual Evoked Potential Recording in Rodents Yuyi You, Alexander Klistorner, and Stuart L. Graham 17. The Visual Evoked Potential in Humans Stuart L. Graham and Alexander Klistorner 18. Improving Motor Activation Patterns after Stroke with Wii-Based Movement Therapy Angelica G. Thompson-Butel, Sarah E. Scheuer, and Penelope A. McNulty
