Description
The cerebral cytoskeleton, considered to be the working horse in brain tissues, becomes particularly important when degenerative diseases of the nervous system are considered. The Cytoskeleton: Imaging, Isolation, and Interaction assembles chapters on general aspects of the cytoskeleton, which are helpful for polishing knowledge of the cytoskeleton in a brief and informative way, and these pieces are flanked by a collection of detailed protocols on diverse emerging techniques including in vivo and in vitro imaging of the cytoskeleton at work as well as proteomics methodologies addressing preparative strategies for the isolation of cytoskeletal components of the central nervous system. Written for the popular Neuromethods series, this work contains the kind of meticulous descriptions and implementation advice to guarantee successful results. Authoritative and cutting-edge, The Cytoskeleton: Imaging, Isolation, and Interaction provides a compilation of papers written by a team of experts which aims to strengthen our efforts at overcoming at least some of the fatal outcomes of the diseased brain cytoskeleton. Part I: Imaging 1. Fluorescence Proteins and Time-Lapse Imaging of the Cytoskeleton Carsten Theiss and Karl Meller 2. Visualizing In Vitro Trafficking John Murray Part II: Interaction, Dynamics, and Proteomics 3. Interaction of the Glycocalyx with the Actin Cytoskeleton Mia M. Thi, Eno E. Ebong, David C. Spray, and Sylvia O. Suadicani 4. Cytoskeleton Dynamics and Binding Factors Elena E. Grintsevich and Emil Reisler 5. Principles of Proteomic Approaches to the Cytoskeleton Christina Loosse, Katrin Marcus, and Bodo Schoenebeck Part III: The Neuronal Cytoskeleton 6. Analyzing the Cytoskeletal Transcriptome: Sex Differences in Rat Hypothalamus Sanda Iacobas, Genevieve Neal-Perry, and Dumitru Andrei Iacobas 7. Proteomic Approaches for the Study of Electrical Synapses and Associated Protein-Interaction Complexes Cantas Alev, Georg Zoidl, and Rolf Dermietzel 8. The Cytoskeleton as a Pharmacological Target in Neurodegenerative Diseases Saar Oz and Illana Gozes 9. Neurofilaments: Properties, Functions, and Regulation Rodolphe Perrot and Joel Eyer 10. From an Axon into a Growth Cone after Axotomy: A Model for Cytoskeletal Dynamics Micha E. Spira and Hadas Erez 11. Isolation of the Postsynaptic Density: A Specialization of the Subsynaptic Cytoskeleton Karl-Heinz Smalla, Patricia Klemmer, and Ursula Wyneken Part IV: Astroglia and the Cytoskeleton 12. Cell-to-Cell Communication in Astroglia and the Cytoskeletal Impact Georg Zoid, Stefan Olk, Andrey Tuchinowitz, and Rolf Dermietzel 13. Reactive Astrocytes, Astrocyte Intermediate Filament Proteins, and Their Role in the Disease Pathogenesis Milos Pekny, Michelle Porritt, Yolanda de Pablo, Marcela Pekna, and Ulrika Wilhelmsson Part V: Oligodendroglia and Myelin 14. Microtubules and Associated Proteins in Oligodendrocytes, the Myelin Forming Cells of the Central Nervous System Christiane Richter-Landsberg 15. Myelin Proteome Analysis: Methods and Implications for the Myelin Cytoskeleton Olaf Jahn, Stefan Tenzer, Nicole Bartsch, Julia Patzig, and Hauke B. Werner Sanda Iacobas, Genevieve Neal-Perry, and Dumitru Andrei Iacobas 7. Proteomic Approaches for the Study of Electrical Synapses and Associated Protein-Interaction Complexes Cantas Alev, Georg Zoidl, and Rolf Dermietzel 8. The Cytoskeleton as a Pharmacological Target in Neurodegenerative Diseases Saar Oz and Illana Gozes 9. Neurofilaments: Properties, Functions, and Regulation Rodolphe Perrot and Joel Eyer 10. From an Axon into a Growth Cone after Axotomy: A Model for Cytoskeletal Dynamics Micha E. Spira and Hadas Erez 11. Isolation of the Postsynaptic Density: A Specialization of the Subsynaptic Cytoskeleton Karl-Heinz Smalla, Patricia Klemmer, and Ursula Wyneken Part IV: Astroglia and the Cytoskeleton 12. Cell-to-Cell Communication in Astroglia and the Cytoskeletal Impact Georg Zoid, Stefan Olk, Andrey Tuchinowitz, and Rolf Dermietzel 13. Reactive Astrocytes, Astrocyte Intermediate Filament Proteins, and Their Role in the Disease Pathogenesis Milos Pekny, Michelle Porritt, Yolanda de Pablo, Marcela Pekna, and Ulrika Wilhelmsson Part V: Oligodendroglia and Myelin 14. Microtubules and Associated Proteins in Oligodendrocytes, the Myelin Forming Cells of the Central Nervous System Christiane Richter-Landsberg 15. Myelin Proteome Analysis: Methods and Implications for the Myelin Cytoskeleton Olaf Jahn, Stefan Tenzer, Nicole Bartsch, Julia Patzig, and Hauke B. Werner Saar Oz and Illana Gozes 9. Neurofilaments: Properties, Functions, and Regulation Rodolphe Perrot and Joel Eyer 10. From an Axon into a Growth Cone after Axotomy: A Model for Cytoskeletal Dynamics Micha E. Spira and Hadas Erez 11. Isolation of the Postsynaptic Density: A Specialization of the Subsynaptic Cytoskeleton Karl-Heinz Smalla, Patricia Klemmer, and Ursula Wyneken Part IV: Astroglia and the Cytoskeleton 12. Cell-to-Cell Communication in Astroglia and the Cytoskeletal Impact Georg Zoid, Stefan Olk, Andrey Tuchinowitz, and Rolf Dermietzel 13. Reactive Astrocytes, Astrocyte Intermediate Filament Proteins, and Their Role in the Disease Pathogenesis Milos Pekny, Michelle Porritt, Yolanda de Pablo, Marcela Pekna, and Ulrika Wilhelmsson Part V: Oligodendroglia and Myelin 14. Microtubules and Associated Proteins in Oligodendrocytes, the Myelin Forming Cells of the Central Nervous System Christiane Richter-Landsberg 15. Myelin Proteome Analysis: Methods and Implications for the Myelin Cytoskeleton Olaf Jahn, Stefan Tenzer, Nicole Bartsch, Julia Patzig, and Hauke B. Werner 11. Isolation of the Postsynaptic Density: A Specialization of the Subsynaptic Cytoskeleton Karl-Heinz Smalla, Patricia Klemmer, and Ursula Wyneken Part IV: Astroglia and the Cytoskeleton 12. Cell-to-Cell Communication in Astroglia and the Cytoskeletal Impact Georg Zoid, Stefan Olk, Andrey Tuchinowitz, and Rolf Dermietzel 13. Reactive Astrocytes, Astrocyte Intermediate Filament Proteins, and Their Role in the Disease Pathogenesis Milos Pekny, Michelle Porritt, Yolanda de Pablo, Marcela Pekna, and Ulrika Wilhelmsson Part V: Oligodendroglia and Myelin 14. Microtubules and Associated Proteins in Oligodendrocytes, the Myelin Forming Cells of the Central Nervous System Christiane Richter-Landsberg 15. Myelin Proteome Analysis: Methods and Implications for the Myelin Cytoskeleton Olaf Jahn, Stefan Tenzer, Nicole Bartsch, Julia Patzig, and Hauke B. Werner Georg Zoid, Stefan Olk, Andrey Tuchinowitz, and Rolf Dermietzel 13. Reactive Astrocytes, Astrocyte Intermediate Filament Proteins, and Their Role in the Disease Pathogenesis Milos Pekny, Michelle Porritt, Yolanda de Pablo, Marcela Pekna, and Ulrika Wilhelmsson Part V: Oligodendroglia and Myelin 14. Microtubules and Associated Proteins in Oligodendrocytes, the Myelin Forming Cells of the Central Nervous System Christiane Richter-Landsberg 15. Myelin Proteome Analysis: Methods and Implications for the Myelin Cytoskeleton Olaf Jahn, Stefan Tenzer, Nicole Bartsch, Julia Patzig, and Hauke B. Werner 15. Myelin Proteome Analysis: Methods and Implications for the Myelin Cytoskeleton Olaf Jahn, Stefan Tenzer, Nicole Bartsch, Julia Patzig, and Hauke B. Werner Olaf Jahn, Stefan Tenzer, Nicole Bartsch, Julia Patzig, and Hauke B. Werner
