Description
After decades of research in clinical transplantation, new techniques have been developed that permit a further understanding of the immune mechanisms underlying immune recognition of allografts and a more accurate and thorough evaluation of compatibility between donors and recipients. The second edition of Transplantation Immunology: Methods and Protocols expands upon the previous edition with current, detailed methods in transplantation immunology. The new methods chapters cover four major areas that are being applied in compatibility evaluations and ongoing transplantation immunology research. Seven overview chapters provide reviews of the molecular basis for alloreactivity, current understanding of humoral and cellular mechanisms, as well as new developments in thoracic organ transplantation, composite tissue transplantation and in the transplantation of sensitized patients. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Transplantation Immunology: Methods and Protocol, Second Edition is devoted to transplantation immunology, both in the practice of compatibility testing and in transplantation research. PART I: Overview Chapters1. Alloreactivity Sidonia B.G. Eckle, Jamie Rossjohn, and James McCluskey 2. Antibodies in Transplantation: The Effects of HLA and Non-HLA Antibody Binding and Mechanisms of Injury Nicole M. Valenzuela and Elaine F. Reed 3. Cell Mediated Rejection Kathryn J Wood, Masaaki Zaitsu and Ryoichi Goto 4. Transplantation Tolerance Colin Brinkman, Bryna Burrell, Joseph Scalea, and Jonathan Bromberg 5. Composite Tissue Transplantation Gerald Brandacher 6. Transplantation of the Sensitized Patient: Histocompatibility Testing Robert A. Montgomery, Mary S. Leffell, and Andrea A. Zachary 7. Thoracic Organ Transplantation: Laboratory Methods Jignesh K. Patel and Jon A. Kobashigawa PART II: Methods Chapters 8. HLA Typing by Sequence Specific Primers Mike Bunce, Biofortuna and Ben Passey, Biofortuna 9. Human Leukocyte Antigen (HLA) Typing By DNA Sequencing Ana Lazaro, Bin Tu, Ruyan Yang, Yi Xiao, Kanthi Kariyawasam, Jennifer Ng, Carolyn and Katovich Hurley 10. Next-Generation HLA Sequencing using the 454 GS FLX System Elizabeth A. Trachtenberg and Cherie L. Holcomb 11. Chimerism Testing by Quantitative PCR Using Indel Markers Ketevan Gendzekhadze, Laima Gaidulis, and David Senitzer 12. Gene-specific PCR Typing of Killer Cell Immunoglobulin-like Receptors (KIR) Raja Rajalingam and Elham Ashouri 13. Complement-dependent Cytotoxicity Crossmatch Jeremy Ryan Pea, Donna Fitzpatrick, and Susan L. Saidman 14. Lymphocyte Crossmatching by Flow Cytometry Robert A. Bray 15. Solid Phase Assay Measuring C4d Deposition to Determine Complement Fixation by HLA Specific Antibodies John D Smith and Marlene L Rose 16. C1q Assay for the Detection of Complement Fixing Antibody to HLA Antigens Ge Chen and Dolly B. Tyan 17. Tetramer Staining for the Detection of HLA-specific B cells Donna P. Lucas, Mary S. Leffell, and Andrea A. Zachary 18. A Flow Cytometric Crossmatch Test Using Endothelial Precursor Cells Isolated from Peripheral Blood Annette M. Jackson, Donna P. Lucas, Jessica L. Badders 19. The Detection of Antibodies to the Angiotensin II-Type 1 Receptor in Transplantation Duska Dragun 20. Detection of Antibodies to Self-antigens (K-alpha 1 Tubulin, Collagen I, II, IV and V Myosin and Vimentin) by Enzyme-Linked Immunosorbent Assay (ELISA) Nayan J. Sarma, Venkataswarup Tiriveedhi, and T. Mohanakumar 21. Cylex ImmuKnow Cell Function Assay Adriana Zeevi and John Lunz 22. Detection of Intracellular Cytokines Nancy L. Reinsmoen and Chih-Hung Lai 23. Tolerogenic Dendritic Cells and Induction of T Suppresson Cells in Transplant Recipients George Vlad and Nicole Suciu-Foca 24. Discovery and Customized Validation of Antibody Targets by Protein Arrays and Indirect ELISA Tara K. Sigdel, and Minnie. M. Sarwal 25. RNA Purification and Expression Analysis using Microarrays and RNA Deep Sequencing Steven R. Head, Tony Mondala, Terri Gelbart, and Phillip Ordoukhanian
